Our lab is developing new tools and techniques that facilitate the study of the structure and dynamics of proteins and protein complexes, functional units that include one or more proteins. Our studies rely on nuclear magnetic resonance (NMR) spectroscopy, a research approach that relies on the magnetic properties of the nucleus of certain atoms to determine physical and chemical properties of the molecules in which they are contained.
Currently, we are especially interested in developing novel NMR approaches to detect and visualize short-lived, sparsely populated states that are invisible to conventional biophysical and structural techniques. Such species play a critical role in recognition and molecular assembly. Other interests include the development of hybrid strategies to solve the structures of large (> 100 kDa) complexes using a combination of NMR and solution X-ray scattering techniques.
Our research extends the use of NMR to studies that were previously impossible. For instance, NMR can be used for larger structures. The many proteins and protein complexes we describe are resulting in new insights into fundamental cell operations. Our many advances in the use of NMR also include the development of mathematical algorithms and computational techniques that are making analysis of NMR data faster and more efficient.