U.S. Department of Health and Human Services
Robert Craigie
 

 Contact Info

 
Tel: 301-496-4081
Email: bobc@helix.nih.gov
 

 Select Experience

 
  • Ph.D.London1982
  • B.S.London1978
 

 Related Links

 
    Specialties
    • Molecular Biology/Biochemistry

    ​Research Images

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    TitleDescriptionImage
    Domains of HIV-1 integraseThis image depicts domains of HIV-1 integrase.  HIV-1 integrase comprises three structural domains.  The central domain is highly conserved among retroviruses, retrotransposons, and many DNA transposons. It contains the active site for polynucleotidyl transfer.  The N-terminal domain contains the HHCC motif that binds zinc. The C-terminal domain is less well conserved.Domains of HIV-1 integraseEnlarge
    Structures of the individual domains of HIV-1 integraseThis image depicts structures of the individual domains of HIV-1 integrase.  A major goal is to understand how these domains are organized in complex with viral DNA (intasomes).  Current inhibitors of HIV integrase bind to intasomes rather than free integrase proteins.  Understanding the mechanism inhibition and the development of resistance requires high-resolution structures of intasomes.Structures of the individual domains of HIV-1 integraseEnlarge
    HIV-1 intrasomes visualized by atomic force microscopyHIV-1 intasomes are visualized by atomic force microscopy.  A tetramer of integrase (white spot) bridges a pair of DNA ends.HIV-1 intrasomes visualized by atomic force microscopyEnlarge
    Condensation of DNA by barrier-to-autointegration factor (BAF)Condensation of DNA by barrier-to-autointegration factor (BAF) is shown.  Single DNA molecules are extended by buffer flow and visualized by fluorescence microscopy.  At time zero BAF is introduced into the flow cell.   The molecules compact into a ball as a result of DNA bridging by BAF. Depicted are (a) a molecule attached to surface at one end only, (b) a molecule attached at both ends, (C) two molecules attached side-by-side “zipper” together, and (d) other examples of molecules attached at one end.Condensation of DNA by barrier-to-autointegration factor (BAF)Enlarge