U.S. Department of Health and Human Services
Stoney Simons Jr

 Contact Info

Tel: 301-496-6796
Email: stoney.simons@nih.gov

 Select Experience

  • Postdoctoral FellowUniversity of California, San Francisco1972-1975
  • Ph.D.Harvard University1972
  • B.A.Princeton University1967

 Related Links


    • Cell Biology/Cell Signaling
    • Molecular Pharmacology/Toxicology


    A selection of recent and significant publications can be viewed below.

    Blackford JA Jr, Brimacombe KR, Dougherty EJ, Pradhan M, Shen M, Li Z, Auld DS, Chow CC, Austin CP, Simons SS Jr. Research Resource: Modulators of glucocorticoid receptor activity identified by a new high-throughput screening assay. Mol Endocrinol. 2014 Jul; 28 (28; 7; 2014 Jul):me20141069. [Full Text/Abstract]
    Zhu R, Lu X, Pradhan M, Armstrong SP, Storchan GB, Chow CC, Simons SS Jr. A kinase-independent activity of cdk9 modulates glucocorticoid receptor-mediated gene induction. Biochemistry. 2014 Mar 25; 53 (2014 Mar 25; 53; 11):1753-67. [Full Text/Abstract]
    Simons SS Jr, Edwards DP, Kumar R. Dynamic Structures of Nuclear Hormone Receptors: New Promises and Challenges. Mol Endocrinol. 2014 Feb; 28 (28; 2; 2014 Feb):173-82. [Full Text/Abstract]
    Luo M, Lu X, Zhu R, Zhang Z, Chow CC, Li R, Simons SS Jr. A Conserved Protein Motif Is Required for Full Modulatory Activity of Negative Elongation Factor Subunits NELF-A and NELF-B in Modifying Glucocorticoid Receptor-regulated Gene Induction Properties. J Biol Chem. 2013 Nov 22; 288 (288; 2013 Nov 22; 47):34055-72. [Full Text/Abstract]
    Simons SS Jr, Kumar R. Variable steroid receptor responses: Intrinsically disordered AF1 is the key. Mol Cell Endocrinol. 2013 Aug 25; 376 (376; 2013 Aug 25; 1-2):81-4. [Full Text/Abstract]
    Lee GS, He Y, Dougherty EJ, Jimenez-Movilla M, Avella M, Grullon S, Sharlin DS, Guo C, Blackford JA Jr, Awasthi S, Zhang Z, Armstrong SP, London EC, Chen W, Dean J, Simons SS Jr. Disruption of Ttll5/stamp gene (tubulin tyrosine ligase-like protein 5/SRC-1 and TIF2-associated modulatory protein gene) in male mice causes sperm malformation and infertility. J Biol Chem. 2013 May 24; 288 (2013 May 24; 288; 21):15167-80. [Full Text/Abstract]
    Zhang Z, Sun Y, Cho YW, Chow CC, Simons SS Jr. PA1 protein, a new competitive decelerator acting at more than one step to impede glucocorticoid receptor-mediated transactivation. J Biol Chem. 2013 Jan 4; 288 (2013 Jan 4; 288; 1):42-58. [Full Text/Abstract]
    Khan SH, Awasthi S, Guo C, Goswami D, Ling J, Griffin PR, Simons SS Jr, Kumar R. Binding of the N-terminal region of coactivator TIF2 to the intrinsically disordered AF1 domain of the glucocorticoid receptor is accompanied by conformational reorganizations. J Biol Chem. 2012 Dec 28; 287 (2012 Dec 28; 287; 53):44546-60. [Full Text/Abstract]
    Blackford JA Jr, Guo C, Zhu R, Dougherty EJ, Chow CC, Simons SS Jr. Identification of location and kinetically defined mechanism of cofactors and reporter genes in the cascade of steroid-regulated transactivation. J Biol Chem. 2012 Nov 30; 287 (2012 Nov 30; 49; 287):40982-95. [Full Text/Abstract]
    Awasthi S, Simons SS Jr. Separate regions of glucocorticoid receptor, coactivator TIF2, and comodulator STAMP modify different parameters of glucocorticoid-mediated gene induction. Mol Cell Endocrinol. 2012 May 15; 355 (2012 May 15; 355; 1):121-34. [Full Text/Abstract]
    Simons SS Jr, Chow CC. The road less traveled: new views of steroid receptor action from the path of dose-response curves. Mol Cell Endocrinol. 2012 Jan 30; 348 (348; 2; 2012 Jan 30):373-82. [Full Text/Abstract]
    Dougherty EJ, Guo C, Simons SS Jr, Chow CC. Deducing the temporal order of cofactor function in ligand-regulated gene transcription: theory and experimental verification. PLoS One. 2012; 7 (2012; 7; 1):e30225. [Full Text/Abstract]
    Lee GS, Simons SS Jr. Ligand binding domain mutations of the glucocorticoid receptor selectively modify the effects with, but not binding of, cofactors. Biochemistry. 2011 Jan 25; 50 (50; 2011 Jan 25; 3):356-66. [Full Text/Abstract]
    Chow CC, Ong KM, Dougherty EJ, Simons SS Jr. Inferring mechanisms from dose-response curves. Methods Enzymol. 2011; 487 (2011; 487):465-83. [Full Text/Abstract]
    Simons SS Jr. Glucocorticoid receptor cofactors as therapeutic targets. Curr Opin Pharmacol. 2010 Dec; 10 (6; 2010 Dec; 10):613-9. [Full Text/Abstract]
    Ong KM, Blackford JA Jr, Kagan BL, Simons SS Jr, Chow CC. A theoretical framework for gene induction and experimental comparisons. Proc Natl Acad Sci U S A. 2010 Apr 13; 107 (107; 2010 Apr 13; 15):7107-12. [Full Text/Abstract]
    He Y, Blackford JA Jr, Kohn EC, Simons SS Jr. STAMP alters the growth of transformed and ovarian cancer cells. BMC Cancer. 2010 Apr 7; 10 (2010 Apr 7; 10):128. [Full Text/Abstract]
    Luo M, Simons SS Jr. Modulation of glucocorticoid receptor induction properties by cofactors in peripheral blood mononuclear cells. Hum Immunol. 2009 Oct; 70 (2009 Oct; 10; 70):785-9. [Full Text/Abstract]
    Ronacher K, Hadley K, Avenant C, Stubsrud E, Simons SS Jr, Louw A, Hapgood JP. Ligand-selective transactivation and transrepression via the glucocorticoid receptor: role of cofactor interaction. Mol Cell Endocrinol. 2009 Feb 27; 299 (299; 2; 2009 Feb 27):219-31. [Full Text/Abstract]
    Simons SS Jr. What goes on behind closed doors: physiological versus pharmacological steroid hormone actions. Bioessays. 2008 Aug; 30 (30; 8; 2008 Aug):744-56. [Full Text/Abstract]
    Tao YG, Xu Y, Xu HE, Simons SS Jr. Mutations of glucocorticoid receptor differentially affect AF2 domain activity in a steroid-selective manner to alter the potency and efficacy of gene induction and repression. Biochemistry. 2008 Jul 22; 47 (2008 Jul 22; 29; 47):7648-62. [Full Text/Abstract]
    Suino-Powell K, Xu Y, Zhang C, Tao YG, Tolbert WD, Simons SS Jr, Xu HE. Doubling the size of the glucocorticoid receptor ligand binding pocket by deacylcortivazol. Mol Cell Biol. 2008 Mar; 28 (28; 6; 2008 Mar):1915-23. [Full Text/Abstract]
    Szapary D, Song LN, He Y, Simons SS Jr. Differential modulation of glucocorticoid and progesterone receptor transactivation. Mol Cell Endocrinol. 2008 Feb 13; 283 (2008 Feb 13; 1-2; 283):114-26. [Full Text/Abstract]
    Wang D, Wang Q, Awasthi S, Simons SS Jr. Amino-terminal domain of TIF2 is involved in competing for corepressor binding to glucocorticoid and progesterone receptors. Biochemistry. 2007 Jul 10; 46 (46; 2007 Jul 10; 27):8036-49. [Full Text/Abstract]
    He Y, Simons SS Jr. STAMP, a novel predicted factor assisting TIF2 actions in glucocorticoid receptor-mediated induction and repression. Mol Cell Biol. 2007 Feb; 27 (27; 4; 2007 Feb):1467-85. [Full Text/Abstract]
    Kim Y, Sun Y, Chow C, Pommier YG, Simons SS Jr. Effects of acetylation, polymerase phosphorylation, and DNA unwinding in glucocorticoid receptor transactivation. J Steroid Biochem Mol Biol. 2006 Jul; 100 (100; 1-3; 2006 Jul):3-17. [Full Text/Abstract]
    Simons SS Jr. How much is enough? Modulation of dose-response curve for steroid receptor-regulated gene expression by changing concentrations of transcription factor. Curr Top Med Chem. 2006; 6 (6; 3; 2006):271-85. [Full Text/Abstract]
    Wang D, Simons SS Jr. Corepressor binding to progesterone and glucocorticoid receptors involves the activation function-1 domain and is inhibited by molybdate. Mol Endocrinol. 2005 Jun; 19 (6; 19; 2005 Jun):1483-500. [Full Text/Abstract]
    Cho S, Blackford JA Jr, Simons SS Jr. Role of activation function domain-1, DNA binding, and coactivator GRIP1 in the expression of partial agonist activity of glucocorticoid receptor-antagonist complexes. Biochemistry. 2005 Mar 8; 44 (2005 Mar 8; 9; 44):3547-61. [Full Text/Abstract]
    Cho S, Kagan BL, Blackford JA Jr, Szapary D, Simons SS Jr. Glucocorticoid receptor ligand binding domain is sufficient for the modulation of glucocorticoid induction properties by homologous receptors, coactivator transcription intermediary factor 2, and Ubc9. Mol Endocrinol. 2005 Feb; 19 (2005 Feb; 19; 2):290-311. [Full Text/Abstract]
    Chen J, Blackford JA Jr, Simons SS Jr. PCR expression mutagenesis: a high-throughput mutation assay applied to the glucocorticoid receptor ligand-binding domain. Biochem Biophys Res Commun. 2004 Sep 3; 321 (321; 2004 Sep 3; 4):893-9. [Full Text/Abstract]
    Wang Q, Anzick S, Richter WF, Meltzer P, Simons SS Jr. Modulation of transcriptional sensitivity of mineralocorticoid and estrogen receptors. J Steroid Biochem Mol Biol. 2004 Aug; 91 (2004 Aug; 91; 4-5):197-210. [Full Text/Abstract]
    Wang Q, Blackford JA Jr, Song LN, Huang Y, Cho S, Simons SS Jr. Equilibrium interactions of corepressors and coactivators with agonist and antagonist complexes of glucocorticoid receptors. Mol Endocrinol. 2004 Jun; 18 (2004 Jun; 6; 18):1376-95. [Full Text/Abstract]
    Chen J, He Y, Simons SS Jr. Structure/activity relationships for GMEB-2: the second member of the glucocorticoid modulatory element-binding complex. Biochemistry. 2004 Jan 13; 43 (43; 2004 Jan 13; 1):245-55. [Full Text/Abstract]
    Murphy PJ, Morishima Y, Chen H, Galigniana MD, Mansfield JF, Simons SS Jr, Pratt WB. Visualization and mechanism of assembly of a glucocorticoid receptor.Hsp70 complex that is primed for subsequent Hsp90-dependent opening of the steroid binding cleft. J Biol Chem. 2003 Sep 12; 278 (278; 37; 2003 Sep 12):34764-73. [Full Text/Abstract]
    Anzick SL, Azorsa DO, Simons SS Jr, Meltzer PS. Phenotypic alterations in breast cancer cells overexpressing the nuclear receptor co-activator AIB1. BMC Cancer. 2003 Sep 10; 3 (3; 2003 Sep 10):22. [Full Text/Abstract]
    Webster JI, Tonelli LH, Moayeri M, Simons SS Jr, Leppla SH, Sternberg EM. Anthrax lethal factor represses glucocorticoid and progesterone receptor activity. Proc Natl Acad Sci U S A. 2003 May 13; 100 (100; 10; 2003 May 13):5706-11. [Full Text/Abstract]
    Chen S, Simons SS Jr. A second pathway for modulating glucocorticoid receptor transactivation properties. Mol Cell Endocrinol. 2003 Jan 31; 199 (1-2; 2003 Jan 31; 199):129-42. [Full Text/Abstract]
    He Y, Szapary D, Simons SS Jr. Modulation of induction properties of glucocorticoid receptor-agonist and -antagonist complexes by coactivators involves binding to receptors but is independent of ability of coactivators to augment transactivation. J Biol Chem. 2002 Dec 20; 277 (277; 2002 Dec 20; 51):49256-66. [Full Text/Abstract]
    Kaul S, Murphy PJ, Chen J, Brown L, Pratt WB, Simons SS Jr. Mutations at positions 547-553 of rat glucocorticoid receptors reveal that hsp90 binding requires the presence, but not defined composition, of a seven-amino acid sequence at the amino terminus of the ligand binding domain. J Biol Chem. 2002 Sep 27; 277 (277; 39; 2002 Sep 27):36223-32. [Full Text/Abstract]
    Chen J, Kaul S, Simons SS Jr. Structure/activity elements of the multifunctional protein, GMEB-1. Characterization of domains relevant for the modulation of glucocorticoid receptor transactivation properties. J Biol Chem. 2002 Jun 14; 277 (277; 24; 2002 Jun 14):22053-62. [Full Text/Abstract]
    Kaul S, Blackford JA Jr, Cho S, Simons SS Jr. Ubc9 is a novel modulator of the induction properties of glucocorticoid receptors. J Biol Chem. 2002 Apr 12; 277 (277; 15; 2002 Apr 12):12541-9. [Full Text/Abstract]
    Song LN, Huse B, Rusconi S, Simons SS Jr. Transactivation specificity of glucocorticoid versus progesterone receptors. Role of functionally different interactions of transcription factors with amino- and carboxyl-terminal receptor domains. J Biol Chem. 2001 Jul 6; 276 (2001 Jul 6; 276; 27):24806-16. [Full Text/Abstract]
    Giannoukos G, Szapary D, Smith CL, Meeker JE, Simons SS Jr. New antiprogestins with partial agonist activity: potential selective progesterone receptor modulators (SPRMs) and probes for receptor- and coregulator-induced changes in progesterone receptor induction properties. Mol Endocrinol. 2001 Feb; 15 (2; 2001 Feb; 15):255-70. [Full Text/Abstract]
    Chen S, Sarlis NJ, Simons SS Jr. Evidence for a common step in three different processes for modulating the kinetic properties of glucocorticoid receptor-induced gene transcription. J Biol Chem. 2000 Sep 29; 275 (275; 2000 Sep 29; 39):30106-17. [Full Text/Abstract]
    Kaul S, Blackford JA Jr, Chen J, Ogryzko VV, Simons SS Jr. Properties of the glucocorticoid modulatory element binding proteins GMEB-1 and -2: potential new modifiers of glucocorticoid receptor transactivation and members of the family of KDWK proteins. Mol Endocrinol. 2000 Jul; 14 (14; 7; 2000 Jul):1010-27. [Full Text/Abstract]
    Zeng H, Plisov SY, Simons SS Jr. Ability of the glucocorticoid modulatory element to modify glucocorticoid receptor transactivation indicates parallel pathways for the expression of glucocorticoid modulatory element and glucocorticoid response element activities. Mol Cell Endocrinol. 2000 Apr 25; 162 (2000 Apr 25; 1-2; 162):221-34. [Full Text/Abstract]
    Zeng H, Kaul S, Simons SS Jr. Genomic organization of human GMEB-1 and rat GMEB-2: structural conservation of two multifunctional proteins. Nucleic Acids Res. 2000 Apr 15; 28 (2000 Apr 15; 28; 8):1819-29. [Full Text/Abstract]
    Huang Y, Simons SS Jr. Functional analysis of R651 mutations in the putative helix 6 of rat glucocorticoid receptors. Mol Cell Endocrinol. 1999 Dec 20; 158 (1-2; 158; 1999 Dec 20):117-30. [Full Text/Abstract]
    Giannoukos G, Silverstein AM, Pratt WB, Simons SS Jr. The seven amino acids (547-553) of rat glucocorticoid receptor required for steroid and hsp90 binding contain a functionally independent LXXLL motif that is critical for steroid binding. J Biol Chem. 1999 Dec 17; 274 (1999 Dec 17; 51; 274):36527-36. [Full Text/Abstract]