B.S., Furman University, 1983
M.S., Princeton University, 1986
Ph.D., Frankfurt University, Germany, 1990
Research Statement
Our research focuses on the structure determination of integral membrane proteins, with emphasis on ligand recognition, transmembrane signalling, and transport of small and large molecules. We study bacterial outer membrane proteins as possible vaccine targets and bacterial inner membrane proteins as model systems for cellular processes such as nutrient transport. Methods used include heterologous overexpression of membrane proteins, biochemical characterisation, crystallisation, and structure determination by X-ray crystallography.
Back to Top
Selected Publications
1. Wally J, Buchanan SK A structural comparison of human serum transferrin and human lactoferrin. Biometals(20): 249-62, 2007. [Full Text/Abstract]
2. Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Nat Struct Mol Biol(14): 1214-1220, 2007. [Full Text/Abstract]
3. Cascales E, Buchanan SK, Duche D, Kleanthous C, Lloubes R, Postle K, Riley M, Slatin S, Cavard D Colicin biology. Microbiol Mol Biol Rev(71): 158-229, 2007. [Full Text/Abstract]
4. Brooks BE, Buchanan SK Signaling mechanisms for activation of extracytoplasmic function (ECF) sigma factors. Biochim Biophys Acta, 2007. [Full Text/Abstract]
5. Buchanan SK, Lukacik P, Grizot S, Ghirlando R, Ali MM, Barnard TJ, Jakes KS, Kienker PK, Esser L Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import. EMBO J(26): 2594-604, 2007. [Full Text/Abstract]
6. Wally J, Halbrooks PJ, Vonrhein C, Rould MA, Everse SJ, Mason AB, Buchanan SK The crystal structure of iron-free human serum transferrin provides insight into inter-lobe communication and receptor binding. J Biol Chem (281): 24934-44, 2006. [Full Text/Abstract]
7. Buchanan SK Bacterial metal detectors. Mol Microbiol (58): 1205-9, 2005. [Full Text/Abstract]
8. Schalk IJ, Yue WW, Buchanan SK Recognition of iron-free siderophores by TonB-dependent iron transporters. Mol Microbiol (54): 14-22, 2004. [Full Text/Abstract]
9. Grizot S Buchanan SK Structure of the OmpA-like domain of RmpM from Neisseria meningitidis. Mol Microbiol (51): 1027-37, 2004. [Full Text/Abstract]
10. Oke M Sarra R Ghirlando R Farnaud S Gorringe AR Evans RW Buchanan SK The plug domain of a neisserial TonB-dependent transporter retains structural integrity in the absence of its transmembrane beta-barrel. FEBS Lett (564): 294-300, 2004. [Full Text/Abstract]
1 2 Next >>
