Smad4 LoxP Mouse
Mutant Mouse: Smad4
Smad4 conditional KO. Inactivation of LoxP-flanked Smad 4 makes it possible to explore the role of Smad 4 in embryonic development of different tissues and in tumorigenesis.
Members of the TGF-b superfamily (which includes Bone Morphogenic Protein and Activin as well as TGF-b ) bind to and activate specific receptors, which activate receptor Smads (Smad 2/3 or Smad 1/5/8), which activate the common SMAD, SMAD 4. Smad4 -/- mice die early in embryogenesis. Floxed Smad4 exon 8 mutants were prepared so that Smad 4 could be conditionally disrupted when the floxed mice were mated with mice expressing a Cre recombinase that is expressed in particular tissues, at a particular time, or both. The conditional knockout strategy makes it possible to explore the role of Smad 4 in embryonic development of different tissues (e.g., neural crest, retina, heart) and in tumorigenesis (pancreatic and head and neck cancers, interactions with oncogenes and tumor suppressors).