Resource

Smad4 LoxP Mouse

Acronym: Mutant Mouse: Smad4 NIDDK Contact: tao@niddk.nih.gov Principal Investigator(s):

Description:

Smad4 conditional KO.  Inactivation of LoxP-flanked Smad 4 makes it possible to explore the role of Smad 4 in embryonic development of different tissues and in tumorigenesis.

Members of the TGF-b superfamily (which includes Bone Morphogenic Protein and Activin as well as TGF-b ) bind to and activate specific receptors, which activate receptor Smads (Smad 2/3 or Smad 1/5/8), which activate the common SMAD, SMAD 4.  Smad4 -/- mice die early in embryogenesis.  Floxed Smad4 exon 8 mutants were prepared so that Smad 4 could be conditionally disrupted when the floxed mice were mated with mice expressing a Cre recombinase that is expressed in particular tissues, at a particular time, or both.  The conditional knockout strategy makes it possible to explore the role of Smad 4 in embryonic development of different tissues (e.g., neural crest, retina, heart) and in tumorigenesis (pancreatic and head and neck cancers, interactions with oncogenes and tumor suppressors).

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