S1pr1 LoxP (S1pr1 tm2Rlp) Mouse

Acronym: Mutant Mouse: S1pr1 NIDDK Contact: Principal Investigator(s): Richard Proia


A conditional knockout mouse for S1pr1

Sphingosine-1-phosphate (S1P) is a potent bioactive lipid that activates a family of G-protein coupled receptors, S1P1, S1P2, S1P3, S1P4 and S1P5. The S1P1 receptor is widely expressed and has critical functions in the immune, cardiovascular and nervous systems.  It is a target for Fingolimod (brand name: Gilenya™), an immunomodulating drug, FDA approved for treating multiple sclerosis. The S1pr1 knockout mouse and the S1pr1 floxed mouse are useful for studying the function of the S1P1 receptor.

Mice with a floxed S1P1 receptor gene were mated with mice expressing Cre-recombinase only in endothelial cells to disrupt the S1P1 gene exclusively in endothelial cells.  The phenotype of the conditional mutant embryos was indistinguishable from the phenotype of S1P1 null embryos in which the S1P1 receptor was disrupted in all cells.  These results indicate that S1P1 receptors in endothelial cells direct the coverage of embryonic blood vessels by vascular smooth muscle cells.

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