U.S. Department of Health and Human Services

Laboratory of Biochemistry and Genetics

Reed B. Wickner, M.D., Chief


A selection of recent and significant publications can be viewed below.

Chattopadhyay MK, Chen W, Tabor H. Escherichia coli glutathionylspermidine synthetase/amidase: phylogeny and effect on regulation of gene expression.FEMS Microbiol Lett. 2013 Jan; 338 (338; 2; 2013 Jan) :132-40. [Full Text/Abstract]
Kelly AC, Shewmaker FP, Kryndushkin D, Wickner RB. Sex, prions, and plasmids in yeast.Proc Natl Acad Sci U S A. 2012 Oct 2; 109 (109; 40; 2012 Oct 2) :E2683-90. [Full Text/Abstract]
Reidy M, Miot M, Masison DC. Prokaryotic chaperones support yeast prions and thermotolerance and define disaggregation machinery interactions.Genetics. 2012 Sep; 192 (2012 Sep; 192; 1) :185-93. [Full Text/Abstract]
Bateman DA, Wickner RB. [PSI+] Prion transmission barriers protect Saccharomyces cerevisiae from infection: intraspecies 'species barriers'.Genetics. 2012 Feb; 190 (2012 Feb; 190; 2) :569-79. [Full Text/Abstract]
Shakes DC, Allen AK, Albert KM, Golden A. emb-1 encodes the APC16 subunit of the Caenorhabditis elegans anaphase-promoting complex.Genetics. 2011 Oct; 189 (2; 189; 2011 Oct) :549-60. [Full Text/Abstract]
Richie CT, Bembenek JN, Chestnut B, Furuta T, Schumacher JM, Wallenfang M, Golden A. Protein phosphatase 5 is a negative regulator of separase function during cortical granule exocytosis in C. elegans.J Cell Sci. 2011 Sep 1; 124 (124; Pt 17; 2011 Sep 1) :2903-13. [Full Text/Abstract]
Sharma D, Masison DC. Single methyl group determines prion propagation and protein degradation activities of yeast heat shock protein (Hsp)-70 chaperones Ssa1p and Ssa2p.Proc Natl Acad Sci U S A. 2011 Aug 16; 108 (108; 33; 2011 Aug 16) :13665-70. [Full Text/Abstract]
Song MH, Liu Y, Anderson DE, Jahng WJ, O'Connell KF. Protein phosphatase 2A-SUR-6/B55 regulates centriole duplication in C. elegans by controlling the levels of centriole assembly factors.Dev Cell. 2011 Apr 19; 20 (20; 4; 2011 Apr 19) :563-71. [Full Text/Abstract]
McGlinchey RP, Kryndushkin D, Wickner RB. Suicidal [PSI+] is a lethal yeast prion.Proc Natl Acad Sci U S A. 2011 Mar 29; 108 (108; 13; 2011 Mar 29) :5337-41. [Full Text/Abstract]
Stein KK, Nesmith JE, Ross BD, Golden A. Functional redundancy of paralogs of an anaphase promoting complex/cyclosome subunit in Caenorhabditis elegans meiosis.Genetics. 2010 Dec; 186 (186; 2010 Dec; 4) :1285-93. [Full Text/Abstract]
Reidy M, Masison DC. Sti1 regulation of Hsp70 and Hsp90 is critical for curing of Saccharomyces cerevisiae [PSI+] prions by Hsp104.Mol Cell Biol. 2010 Jul; 30 (30; 14; 2010 Jul) :3542-52. [Full Text/Abstract]
Peters N, Perez DE, Song MH, Liu Y, Müller-Reichert T, Caron C, Kemphues KJ, O'Connell KF. Control of mitotic and meiotic centriole duplication by the Plk4-related kinase ZYG-1.J Cell Sci. 2010 Mar 1; 123 (2010 Mar 1; 123; Pt 5) :795-805. [Full Text/Abstract]
Kumfer KT, Cook SJ, Squirrell JM, Eliceiri KW, Peel N, O'Connell KF, White JG. CGEF-1 and CHIN-1 regulate CDC-42 activity during asymmetric division in the Caenorhabditis elegans embryo.Mol Biol Cell. 2010 Jan 15; 21 (2010 Jan 15; 2; 21) :266-77. [Full Text/Abstract]
Chattopadhyay MK, Chen W, Poy G, Cam M, Stiles D, Tabor H. Microarray studies on the genes responsive to the addition of spermidine or spermine to a Saccharomyces cerevisiae spermidine synthase mutant.Yeast. 2009 Oct; 26 (2009 Oct; 10; 26) :531-44. [Full Text/Abstract]
Chattopadhyay MK, Tabor CW, Tabor H. Polyamines are not required for aerobic growth of Escherichia coli: preparation of a strain with deletions in all of the genes for polyamine biosynthesis.J Bacteriol. 2009 Sep; 191 (17; 191; 2009 Sep) :5549-52. [Full Text/Abstract]
Sharma D, Martineau CN, Le Dall MT, Reidy M, Masison DC, Kabani M. Function of SSA subfamily of Hsp70 within and across species varies widely in complementing Saccharomyces cerevisiae cell growth and prion propagation.PLoS One. 2009 Aug 14; 4 (2009 Aug 14; 8; 4) :e6644. [Full Text/Abstract]
Golden A, Liu J, Cohen-Fix O. Inactivation of the C. elegans lipin homolog leads to ER disorganization and to defects in the breakdown and reassembly of the nuclear envelope.J Cell Sci. 2009 Jun 15; 122 (Pt 12; 122; 2009 Jun 15) :1970-8. [Full Text/Abstract]
Arur S, Ohmachi M, Nayak S, Hayes M, Miranda A, Hay A, Golden A, Schedl T. Multiple ERK substrates execute single biological processes in Caenorhabditis elegans germ-line development.Proc Natl Acad Sci U S A. 2009 Mar 24; 106 (106; 2009 Mar 24; 12) :4776-81. [Full Text/Abstract]
Edskes HK, McCann LM, Hebert AM, Wickner RB. Prion variants and species barriers among Saccharomyces Ure2 proteins.Genetics. 2009 Mar; 181 (181; 3; 2009 Mar) :1159-67. [Full Text/Abstract]
Song MH, Aravind L, Müller-Reichert T, O'Connell KF. The conserved protein SZY-20 opposes the Plk4-related kinase ZYG-1 to limit centrosome size.Dev Cell. 2008 Dec; 15 (2008 Dec; 6; 15) :901-12. [Full Text/Abstract]
Song MH, Miliaras NB, Peel N, O'Connell KF. Centrioles: some self-assembly required.Curr Opin Cell Biol. 2008 Dec; 20 (20; 2008 Dec; 6) :688-93. [Full Text/Abstract]
Kryndushkin DS, Shewmaker F, Wickner RB. Curing of the [URE3] prion by Btn2p, a Batten disease-related protein.EMBO J. 2008 Oct 22; 27 (20; 2008 Oct 22; 27) :2725-35. [Full Text/Abstract]
Wickner RB, Shewmaker F, Kryndushkin D, Edskes HK. Protein inheritance (prions) based on parallel in-register beta-sheet amyloid structures.Bioessays. 2008 Oct; 30 (30; 2008 Oct; 10) :955-64. [Full Text/Abstract]
Chattopadhyay MK, Park MH, Tabor H. Hypusine modification for growth is the major function of spermidine in Saccharomyces cerevisiae polyamine auxotrophs grown in limiting spermidine.Proc Natl Acad Sci U S A. 2008 May 6; 105 (105; 2008 May 6; 18) :6554-9. [Full Text/Abstract]
Shewmaker F, Ross ED, Tycko R, Wickner RB. Amyloids of shuffled prion domains that form prions have a parallel in-register beta-sheet structure.Biochemistry. 2008 Apr 1; 47 (2008 Apr 1; 13; 47) :4000-7. [Full Text/Abstract]
Wickner RB, Dyda F, Tycko R. Amyloid of Rnq1p, the basis of the [PIN+] prion, has a parallel in-register beta-sheet structure.Proc Natl Acad Sci U S A. 2008 Feb 19; 105 (105; 2008 Feb 19; 7) :2403-8. [Full Text/Abstract]
Baxa U, Wickner RB, Steven AC, Anderson DE, Marekov LN, Yau WM, Tycko R. Characterization of beta-sheet structure in Ure2p1-89 yeast prion fibrils by solid-state nuclear magnetic resonance.Biochemistry. 2007 Nov 13; 46 (46; 2007 Nov 13; 45) :13149-62. [Full Text/Abstract]
Bembenek JN, Richie CT, Squirrell JM, Campbell JM, Eliceiri KW, Poteryaev D, Spang A, Golden A, White JG. Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase.Development. 2007 Nov; 134 (2007 Nov; 134; 21) :3837-48. [Full Text/Abstract]
Kemp CA, Song MH, Addepalli MK, Hunter G, O'Connell K. Suppressors of zyg-1 define regulators of centrosome duplication and nuclear association in Caenorhabditis elegans.Genetics. 2007 May; 176 (2007 May; 176; 1) :95-113. [Full Text/Abstract]
Golden A, O'Connell KF. Silence is golden: combining RNAi and live cell imaging to study cell cycle regulatory genes during Caenorhabditis elegans development.Methods. 2007 Feb; 41 (2; 41; 2007 Feb) :190-7. [Full Text/Abstract]