U.S. Department of Health and Human Services

Laboratory of Biochemistry and Genetics

Reed B. Wickner, M.D., NIH Distinguished Investigator, Chief


A selection of recent and significant publications can be viewed below.

Smith HE, Fabritius AS, Jaramillo-Lambert A, Golden A. Mapping Challenging Mutations by Whole-Genome Sequencing. G3 (Bethesda). 2016 Mar 4; 6 (2016 Mar 4; 6; 5):1297-304. [Full Text/Abstract]
Stein KK, Golden A. The C. elegans eggshell. WormBook. 2015 Dec 30; (2015 Dec 30):1-35. [Full Text/Abstract]
Wickner RB, Kelly AC. Prions are affected by evolution at two levels. Cell Mol Life Sci. 2015 Dec 28; 73 (6; 2015 Dec 28; 73):1131-1144. [Full Text/Abstract]
Reidy M, Sharma R, Roberts BL, Masison DC. Human J-protein DnaJB6b Cures a Subset of Saccharomyces cerevisiae Prions and Selectively Blocks Assembly of Structurally Related Amyloids. J Biol Chem. 2015 Dec 23; 291 (291; 8; 2015 Dec 23):4035-47. [Full Text/Abstract]
Singaravelu G, Rahimi S, Krauchunas A, Rizvi A, Dharia S, Shakes D, Smith H, Golden A, Singson A. Forward Genetics Identifies a Requirement for the Izumo-like Immunoglobulin Superfamily spe-45 Gene in Caenorhabditis elegans Fertilization. Curr Biol. 2015 Dec 6; 25 (25; 2015 Dec 6; 24):3220-4. [Full Text/Abstract]
Choudhary V, Ojha N, Golden A, Prinz WA. A conserved family of proteins facilitates nascent lipid droplet budding from the ER. J Cell Biol. 2015 Oct 26; 211 (211; 2; 2015 Oct 26):261-71. [Full Text/Abstract]
Young DJ, Guydosh NR, Zhang F, Hinnebusch AG, Green R. Rli1/ABCE1 Recycles Terminating Ribosomes and Controls Translation Reinitiation in 3'UTRs In Vivo. Cell. 2015 Aug 13; 162 (4; 162; 2015 Aug 13):872-84. [Full Text/Abstract]
Chattopadhyay MK, Keembiyehetty CN, Chen W, Tabor H. Polyamines Stimulate the Level of the σ38 Subunit (RpoS) of Escherichia coli RNA Polymerase, Resulting in the Induction of the Glutamate Decarboxylase-dependent Acid Response System via the gadE Regulon. J Biol Chem. 2015 May 29; 290 (2015 May 29; 29; 290):17809-21. [Full Text/Abstract]
Andreasson JO, Milic B, Chen GY, Guydosh NR, Hancock WO, Block SM. Examining kinesin processivity within a general gating framework. Elife. 2015 Apr 22; 4 (2015 Apr 22; 4). [Full Text/Abstract]
Woolstenhulme CJ, Guydosh NR, Green R, Buskirk AR. High-precision analysis of translational pausing by ribosome profiling in bacteria lacking EFP. Cell Rep. 2015 Apr 7; 11 (2015 Apr 7; 11; 1):13-21. [Full Text/Abstract]
Jaramillo-Lambert A, Fuchsman AS, Fabritius AS, Smith HE, Golden A. Rapid and Efficient Identification of Caenorhabditis elegans Legacy Mutations Using Hawaiian SNP-Based Mapping and Whole-Genome Sequencing. G3 (Bethesda). 2015 Mar 4; 5 (5; 2015 Mar 4):1007-19. [Full Text/Abstract]
Wickner RB, Shewmaker FP, Bateman DA, Edskes HK, Gorkovskiy A, Dayani Y, Bezsonov EE. Yeast prions: structure, biology, and prion-handling systems. Microbiol Mol Biol Rev. 2015 Mar; 79 (79; 1; 2015 Mar):1-17. [Full Text/Abstract]
Gorkovskiy A, Thurber KR, Tycko R, Wickner RB. Locating folds of the in-register parallel β-sheet of the Sup35p prion domain infectious amyloid. Proc Natl Acad Sci U S A. 2014 Oct 13; 111 (43; 111; 2014 Oct 13):E4615-22. [Full Text/Abstract]
Allen AK, Nesmith JE, Golden A. An RNAi-based suppressor screen identifies interactors of the Myt1 ortholog of Caenorhabditis elegans. G3 (Bethesda). 2014 Oct 8; 4 (2014 Oct 8; 4; 12):2329-43. [Full Text/Abstract]
Reidy M, Sharma R, Shastry S, Roberts BL, Albino-Flores I, Wickner S, Masison DC. Hsp40s specify functions of hsp104 and hsp90 protein chaperone machines. PLoS Genet. 2014 Oct; 10 (2014 Oct; 10):e1004720. [Full Text/Abstract]
Groveman BR, Dolan MA, Taubner LM, Kraus A, Wickner RB, Caughey B. Parallel In-register Intermolecular β-Sheet Architectures for Prion-seeded Prion Protein (PrP) Amyloids. J Biol Chem. 2014 Jul 15; 289 (2014 Jul 15; 35; 289):24129-42. [Full Text/Abstract]
Edskes HK, Khamar HJ, Winchester CL, Greenler AJ, Zhou A, McGlinchey RP, Gorkovskiy A, Wickner RB. Sporadic distribution of prion-forming ability of sup35p from yeasts and fungi. Genetics. 2014 Jul 31; 198 (2; 198; 2014 Jul 31):605-16. [Full Text/Abstract]
Wickner RB, Bezsonov E, Bateman DA. Normal levels of the antiprion proteins Btn2 and Cur1 cure most newly formed [URE3] prion variants. Proc Natl Acad Sci U S A. 2014 Jul 1; 111 (2014 Jul 1; 111; 26):E2711-20. [Full Text/Abstract]
Kelly AC, Busby B, Wickner RB. Effect of domestication on the spread of the [PIN+] prion in Saccharomyces cerevisiae. Genetics. 2014 May 8; 197 (197; 3; 2014 May 8):1007-24. [Full Text/Abstract]
Park YN, Zhao X, Yim YI, Todor H, Ellerbrock R, Reidy M, Eisenberg E, Masison DC, Greene LE. Hsp104 overexpression cures yeast [PSI+] by causing dissolution of the prion seeds. Eukaryot Cell. 2014 May; 13 (2014 May; 13; 5):635-47. [Full Text/Abstract]
Kumar N, Gaur D, Masison DC, Sharma D. The BAG Homology Domain of Snl1 Cures Yeast Prion [URE3] Through Regulation of Hsp70 Chaperones. G3 (Bethesda). 2014 Mar 20; 4 (2014 Mar 20; 4; 3):461-70. [Full Text/Abstract]
Guydosh NR, Green R. Dom34 rescues ribosomes in 3' untranslated regions. Cell. 2014 Feb 27; 156 (156; 2014 Feb 27; 5):950-62. [Full Text/Abstract]
O'Connell KF, Golden A. Confocal Imaging of the Microtubule Cytoskeleton in C. elegans Embryos and Germ Cells. Methods Mol Biol. 2014; 1075 (2014; 1075):257-72. [Full Text/Abstract]
Chattopadhyay MK, Tabor H. Polyamines are critical for the induction of the glutamate decarboxylase-dependent acid resistance system in Escherichia coli. J Biol Chem. 2013 Nov 22; 288 (288; 2013 Nov 22; 47):33559-70. [Full Text/Abstract]
Woolstenhulme CJ, Parajuli S, Healey DW, Valverde DP, Petersen EN, Starosta AL, Guydosh NR, Johnson WE, Wilson DN, Buskirk AR. Nascent peptides that block protein synthesis in bacteria. Proc Natl Acad Sci U S A. 2013 Mar 5; 110 (110; 10; 2013 Mar 5):E878-87. [Full Text/Abstract]
Wickner RB, Fujimura T, Esteban R. Viruses and prions of Saccharomyces cerevisiae. Adv Virus Res. 2013; 86 (86; 2013):1-36. [Full Text/Abstract]
Chattopadhyay MK, Chen W, Tabor H. Escherichia coli glutathionylspermidine synthetase/amidase: phylogeny and effect on regulation of gene expression. FEMS Microbiol Lett. 2013 Jan; 338 (338; 2; 2013 Jan):132-40. [Full Text/Abstract]
Kelly AC, Shewmaker FP, Kryndushkin D, Wickner RB. Sex, prions, and plasmids in yeast. Proc Natl Acad Sci U S A. 2012 Oct 2; 109 (109; 40; 2012 Oct 2):E2683-90. [Full Text/Abstract]
Reidy M, Miot M, Masison DC. Prokaryotic chaperones support yeast prions and thermotolerance and define disaggregation machinery interactions. Genetics. 2012 Sep; 192 (2012 Sep; 192; 1):185-93. [Full Text/Abstract]
Bateman DA, Wickner RB. [PSI+] Prion transmission barriers protect Saccharomyces cerevisiae from infection: intraspecies 'species barriers'. Genetics. 2012 Feb; 190 (2012 Feb; 190; 2):569-79. [Full Text/Abstract]
Shakes DC, Allen AK, Albert KM, Golden A. emb-1 encodes the APC16 subunit of the Caenorhabditis elegans anaphase-promoting complex. Genetics. 2011 Oct; 189 (2; 189; 2011 Oct):549-60. [Full Text/Abstract]
Richie CT, Bembenek JN, Chestnut B, Furuta T, Schumacher JM, Wallenfang M, Golden A. Protein phosphatase 5 is a negative regulator of separase function during cortical granule exocytosis in C. elegans. J Cell Sci. 2011 Sep 1; 124 (124; Pt 17; 2011 Sep 1):2903-13. [Full Text/Abstract]
Sharma D, Masison DC. Single methyl group determines prion propagation and protein degradation activities of yeast heat shock protein (Hsp)-70 chaperones Ssa1p and Ssa2p. Proc Natl Acad Sci U S A. 2011 Aug 16; 108 (108; 33; 2011 Aug 16):13665-70. [Full Text/Abstract]
Song MH, Liu Y, Anderson DE, Jahng WJ, O'Connell KF. Protein phosphatase 2A-SUR-6/B55 regulates centriole duplication in C. elegans by controlling the levels of centriole assembly factors. Dev Cell. 2011 Apr 19; 20 (20; 4; 2011 Apr 19):563-71. [Full Text/Abstract]
McGlinchey RP, Kryndushkin D, Wickner RB. Suicidal [PSI+] is a lethal yeast prion. Proc Natl Acad Sci U S A. 2011 Mar 29; 108 (108; 13; 2011 Mar 29):5337-41. [Full Text/Abstract]
Stein KK, Nesmith JE, Ross BD, Golden A. Functional redundancy of paralogs of an anaphase promoting complex/cyclosome subunit in Caenorhabditis elegans meiosis. Genetics. 2010 Dec; 186 (186; 2010 Dec; 4):1285-93. [Full Text/Abstract]
Reidy M, Masison DC. Sti1 regulation of Hsp70 and Hsp90 is critical for curing of Saccharomyces cerevisiae [PSI+] prions by Hsp104. Mol Cell Biol. 2010 Jul; 30 (30; 14; 2010 Jul):3542-52. [Full Text/Abstract]
Peters N, Perez DE, Song MH, Liu Y, Müller-Reichert T, Caron C, Kemphues KJ, O'Connell KF. Control of mitotic and meiotic centriole duplication by the Plk4-related kinase ZYG-1. J Cell Sci. 2010 Mar 1; 123 (2010 Mar 1; 123; Pt 5):795-805. [Full Text/Abstract]
Kumfer KT, Cook SJ, Squirrell JM, Eliceiri KW, Peel N, O'Connell KF, White JG. CGEF-1 and CHIN-1 regulate CDC-42 activity during asymmetric division in the Caenorhabditis elegans embryo. Mol Biol Cell. 2010 Jan 15; 21 (2010 Jan 15; 2; 21):266-77. [Full Text/Abstract]
Chattopadhyay MK, Chen W, Poy G, Cam M, Stiles D, Tabor H. Microarray studies on the genes responsive to the addition of spermidine or spermine to a Saccharomyces cerevisiae spermidine synthase mutant. Yeast. 2009 Oct; 26 (2009 Oct; 10; 26):531-44. [Full Text/Abstract]
Guydosh NR, Block SM. Direct observation of the binding state of the kinesin head to the microtubule. Nature. 2009 Sep 3; 461 (461; 2009 Sep 3; 7260):125-8. [Full Text/Abstract]
Chattopadhyay MK, Tabor CW, Tabor H. Polyamines are not required for aerobic growth of Escherichia coli: preparation of a strain with deletions in all of the genes for polyamine biosynthesis. J Bacteriol. 2009 Sep; 191 (17; 191; 2009 Sep):5549-52. [Full Text/Abstract]
Golden A, Liu J, Cohen-Fix O. Inactivation of the C. elegans lipin homolog leads to ER disorganization and to defects in the breakdown and reassembly of the nuclear envelope. J Cell Sci. 2009 Jun 15; 122 (Pt 12; 122; 2009 Jun 15):1970-8. [Full Text/Abstract]
Arur S, Ohmachi M, Nayak S, Hayes M, Miranda A, Hay A, Golden A, Schedl T. Multiple ERK substrates execute single biological processes in Caenorhabditis elegans germ-line development. Proc Natl Acad Sci U S A. 2009 Mar 24; 106 (106; 2009 Mar 24; 12):4776-81. [Full Text/Abstract]
Edskes HK, McCann LM, Hebert AM, Wickner RB. Prion variants and species barriers among Saccharomyces Ure2 proteins. Genetics. 2009 Mar; 181 (181; 3; 2009 Mar):1159-67. [Full Text/Abstract]
Song MH, Aravind L, Müller-Reichert T, O'Connell KF. The conserved protein SZY-20 opposes the Plk4-related kinase ZYG-1 to limit centrosome size. Dev Cell. 2008 Dec; 15 (2008 Dec; 6; 15):901-12. [Full Text/Abstract]
Song MH, Miliaras NB, Peel N, O'Connell KF. Centrioles: some self-assembly required. Curr Opin Cell Biol. 2008 Dec; 20 (20; 2008 Dec; 6):688-93. [Full Text/Abstract]
Kryndushkin DS, Shewmaker F, Wickner RB. Curing of the [URE3] prion by Btn2p, a Batten disease-related protein. EMBO J. 2008 Oct 22; 27 (20; 2008 Oct 22; 27):2725-35. [Full Text/Abstract]
Wickner RB, Shewmaker F, Kryndushkin D, Edskes HK. Protein inheritance (prions) based on parallel in-register beta-sheet amyloid structures. Bioessays. 2008 Oct; 30 (30; 2008 Oct; 10):955-64. [Full Text/Abstract]
Chattopadhyay MK, Park MH, Tabor H. Hypusine modification for growth is the major function of spermidine in Saccharomyces cerevisiae polyamine auxotrophs grown in limiting spermidine. Proc Natl Acad Sci U S A. 2008 May 6; 105 (105; 2008 May 6; 18):6554-9. [Full Text/Abstract]