U.S. Department of Health and Human Services

Laboratory of Biochemistry and Genetics

Reed B. Wickner, M.D., NIH Distinguished Investigator, Chief


A selection of recent and significant publications can be viewed below.

Groveman BR, Dolan MA, Taubner LM, Kraus A, Wickner RB, Caughey B. Parallel In-register Intermolecular β-Sheet Architectures for Prion-seeded Prion Protein (PrP) Amyloids. J Biol Chem. 2014 Jul 15; 289 (2014 Jul 15; 35; 289):24129-42. [Full Text/Abstract]
Edskes HK, Khamar HJ, Winchester CL, Greenler AJ, Zhou A, McGlinchey RP, Gorkovskiy A, Wickner RB. Sporadic distribution of prion-forming ability of sup35p from yeasts and fungi. Genetics. 2014 Jul 31; 198 (2; 198; 2014 Jul 31):605-16. [Full Text/Abstract]
Crouch J, Yu JH, Shankar AG, Tabor HK. We Don't Know Her History, Her Background": Adoptive Parents' Perspectives on Whole Genome Sequencing Results. J Genet Couns. [Full Text/Abstract]
Wickner RB, Bezsonov E, Bateman DA. Normal levels of the antiprion proteins Btn2 and Cur1 cure most newly formed [URE3] prion variants. Proc Natl Acad Sci U S A. 2014 Jul 1; 111 (2014 Jul 1; 111; 26):E2711-20. [Full Text/Abstract]
Yu JH, Harrell TM, Jamal SM, Tabor HK, Bamshad MJ. Attitudes of Genetics Professionals Toward the Return of Incidental Results from Exome and Whole-Genome Sequencing. Am J Hum Genet. [Full Text/Abstract]
Jarvik GP, Amendola LM, Berg JS, Brothers K, Clayton EW, Chung W, Evans BJ, Evans JP, Fullerton SM, Gallego CJ, Garrison NA, Gray SW, Holm IA, Kullo IJ, Lehmann LS, McCarty C, Prows CA, Rehm HL, Sharp RR, Salama J, Sanderson S, Van Driest SL, Williams MS, Wolf SM, Wolf WA, eMERGE Act-ROR Committee and CERC Committee, CSER Act-ROR Working Group, Burke W. Return of Genomic Results to Research Participants: The Floor, the Ceiling, and the Choices In Between. Am J Hum Genet. 2014 Jun 5; 94 (94; 6; 2014 Jun 5):818-26. [Full Text/Abstract]
Yu JH, Crouch J, Jamal SM, Bamshad MJ, Tabor HK. Attitudes of non-African American focus group participants toward return of results from exome and whole genome sequencing. Am J Med Genet A. [Full Text/Abstract]
Kelly AC, Busby B, Wickner RB. Effect of Domestication on the Spread of the [PIN+] Prion in Saccharomyces cerevisiae. Genetics. 2014 May 8; 197 (197; 3; 2014 May 8):1007-24. [Full Text/Abstract]
Park YN, Zhao X, Yim YI, Todor H, Ellerbrock R, Reidy M, Eisenberg E, Masison DC, Greene LE. Hsp104 overexpression cures yeast [PSI+] by causing dissolution of the prion seeds. Eukaryot Cell. 2014 May; 13 (2014 May; 13; 5):635-47. [Full Text/Abstract]
Gordon AS, Tabor HK, Johnson AD, Snively BM, Assimes TL, Auer PL, Ioannidis JP, Peters U, Robinson JG, Sucheston LE, Wang D, Sotoodehnia N, Rotter JI, Psaty BM, Jackson RD, Herrington DM, O'Donnell CJ, Reiner AP, Rich SS, Rieder MJ, Bamshad MJ, Nickerson DA, NHLBI GO Exome Sequencing Project. Quantifying rare, deleterious variation in 12 human cytochrome P450 drug-metabolism genes in a large-scale exome dataset. Hum Mol Genet. 2014 Apr 15; 23 (8; 23; 2014 Apr 15):1957-63. [Full Text/Abstract]
McMillin MJ, Beck AE, Chong JX, Shively KM, Buckingham KJ, Gildersleeve HI, Aracena MI, Aylsworth AS, Bitoun P, Carey JC, Clericuzio CL, Crow YJ, Curry CJ, Devriendt K, Everman DB, Fryer A, Gibson K, Giovannucci Uzielli ML, Graham JM Jr, Hall JG, Hecht JT, Heidenreich RA, Hurst JA, Irani S, Krapels IP, Leroy JG, Mowat D, Plant GT, Robertson SP, Schorry EK, Scott RH, Seaver LH, Sherr E, Splitt M, Stewart H, Stumpel C, Temel SG, Weaver DD, Whiteford M, Williams MS, Tabor HK, Smith JD, Shendure J, Nickerson DA, University of Washington Center for Mendelian Genomics, Bamshad MJ. Mutations in PIEZO2 Cause Gordon Syndrome, Marden-Walker Syndrome, and Distal Arthrogryposis Type 5. Am J Hum Genet. [Full Text/Abstract]
Kumar N, Gaur D, Masison DC, Sharma D. The BAG Homology Domain of Snl1 Cures Yeast Prion [URE3] Through Regulation of Hsp70 Chaperones. G3 (Bethesda). 2014 Mar 20; 4 (2014 Mar 20; 4; 3):461-70. [Full Text/Abstract]
Lange LA, Hu Y, Zhang H, Xue C, Schmidt EM, Tang ZZ, Bizon C, Lange EM, Smith JD, Turner EH, Jun G, Kang HM, Peloso G, Auer P, Li KP, Flannick J, Zhang J, Fuchsberger C, Gaulton K, Lindgren C, Locke A, Manning A, Sim X, Rivas MA, Holmen OL, Gottesman O, Lu Y, Ruderfer D, Stahl EA, Duan Q, Li Y, Durda P, Jiao S, Isaacs A, Hofman A, Bis JC, Correa A, Griswold ME, Jakobsdottir J, Smith AV, Schreiner PJ, Feitosa MF, Zhang Q, Huffman JE, Crosby J, Wassel CL, Do R, Franceschini N, Martin LW, Robinson JG, Assimes TL, Crosslin DR, Rosenthal EA, Tsai M, Rieder MJ, Farlow DN, Folsom AR, Lumley T, Fox ER, Carlson CS, Peters U, Jackson RD, van Duijn CM, Uitterlinden AG, Levy D, Rotter JI, Taylor HA, Gudnason V Jr, Siscovick DS, Fornage M, Borecki IB, Hayward C, Rudan I, Chen YE, Bottinger EP, Loos RJ, Sætrom P, Hveem K, Boehnke M, Groop L, McCarthy M, Meitinger T, Ballantyne CM, Gabriel SB, O'Donnell CJ, Post WS, North KE, Reiner AP, Boerwinkle E, Psaty BM, Altshuler D, Kathiresan S, Lin DY, Jarvik GP, Cupples LA, Kooperberg C, Wilson JG, Nickerson DA, Abecasis GR, Rich SS, Tracy RP, Willer CJ, NHLBI Grand Opportunity Exome Sequencing Project. Whole-exome sequencing identifies rare and low-frequency coding variants associated with LDL cholesterol. Am J Hum Genet. 94 (94; 2):233-45. [Full Text/Abstract]
Chattopadhyay MK, Chen W, Tabor H. Escherichia coli glutathionylspermidine synthetase/amidase: phylogeny and effect on regulation of gene expression. FEMS Microbiol Lett. 2013 Jan; 338 (338; 2; 2013 Jan):132-40. [Full Text/Abstract]
Kelly AC, Shewmaker FP, Kryndushkin D, Wickner RB. Sex, prions, and plasmids in yeast. Proc Natl Acad Sci U S A. 2012 Oct 2; 109 (109; 40; 2012 Oct 2):E2683-90. [Full Text/Abstract]
Reidy M, Miot M, Masison DC. Prokaryotic chaperones support yeast prions and thermotolerance and define disaggregation machinery interactions. Genetics. 2012 Sep; 192 (2012 Sep; 192; 1):185-93. [Full Text/Abstract]
Bateman DA, Wickner RB. [PSI+] Prion transmission barriers protect Saccharomyces cerevisiae from infection: intraspecies 'species barriers'. Genetics. 2012 Feb; 190 (2012 Feb; 190; 2):569-79. [Full Text/Abstract]
Shakes DC, Allen AK, Albert KM, Golden A. emb-1 encodes the APC16 subunit of the Caenorhabditis elegans anaphase-promoting complex. Genetics. 2011 Oct; 189 (2; 189; 2011 Oct):549-60. [Full Text/Abstract]
Richie CT, Bembenek JN, Chestnut B, Furuta T, Schumacher JM, Wallenfang M, Golden A. Protein phosphatase 5 is a negative regulator of separase function during cortical granule exocytosis in C. elegans. J Cell Sci. 2011 Sep 1; 124 (124; Pt 17; 2011 Sep 1):2903-13. [Full Text/Abstract]
Sharma D, Masison DC. Single methyl group determines prion propagation and protein degradation activities of yeast heat shock protein (Hsp)-70 chaperones Ssa1p and Ssa2p. Proc Natl Acad Sci U S A. 2011 Aug 16; 108 (108; 33; 2011 Aug 16):13665-70. [Full Text/Abstract]
Song MH, Liu Y, Anderson DE, Jahng WJ, O'Connell KF. Protein phosphatase 2A-SUR-6/B55 regulates centriole duplication in C. elegans by controlling the levels of centriole assembly factors. Dev Cell. 2011 Apr 19; 20 (20; 4; 2011 Apr 19):563-71. [Full Text/Abstract]
McGlinchey RP, Kryndushkin D, Wickner RB. Suicidal [PSI+] is a lethal yeast prion. Proc Natl Acad Sci U S A. 2011 Mar 29; 108 (108; 13; 2011 Mar 29):5337-41. [Full Text/Abstract]
Stein KK, Nesmith JE, Ross BD, Golden A. Functional redundancy of paralogs of an anaphase promoting complex/cyclosome subunit in Caenorhabditis elegans meiosis. Genetics. 2010 Dec; 186 (186; 2010 Dec; 4):1285-93. [Full Text/Abstract]
Reidy M, Masison DC. Sti1 regulation of Hsp70 and Hsp90 is critical for curing of Saccharomyces cerevisiae [PSI+] prions by Hsp104. Mol Cell Biol. 2010 Jul; 30 (30; 14; 2010 Jul):3542-52. [Full Text/Abstract]
Peters N, Perez DE, Song MH, Liu Y, Müller-Reichert T, Caron C, Kemphues KJ, O'Connell KF. Control of mitotic and meiotic centriole duplication by the Plk4-related kinase ZYG-1. J Cell Sci. 2010 Mar 1; 123 (2010 Mar 1; 123; Pt 5):795-805. [Full Text/Abstract]
Kumfer KT, Cook SJ, Squirrell JM, Eliceiri KW, Peel N, O'Connell KF, White JG. CGEF-1 and CHIN-1 regulate CDC-42 activity during asymmetric division in the Caenorhabditis elegans embryo. Mol Biol Cell. 2010 Jan 15; 21 (2010 Jan 15; 2; 21):266-77. [Full Text/Abstract]
Chattopadhyay MK, Chen W, Poy G, Cam M, Stiles D, Tabor H. Microarray studies on the genes responsive to the addition of spermidine or spermine to a Saccharomyces cerevisiae spermidine synthase mutant. Yeast. 2009 Oct; 26 (2009 Oct; 10; 26):531-44. [Full Text/Abstract]
Chattopadhyay MK, Tabor CW, Tabor H. Polyamines are not required for aerobic growth of Escherichia coli: preparation of a strain with deletions in all of the genes for polyamine biosynthesis. J Bacteriol. 2009 Sep; 191 (17; 191; 2009 Sep):5549-52. [Full Text/Abstract]
Sharma D, Martineau CN, Le Dall MT, Reidy M, Masison DC, Kabani M. Function of SSA subfamily of Hsp70 within and across species varies widely in complementing Saccharomyces cerevisiae cell growth and prion propagation. PLoS One. 2009 Aug 14; 4 (2009 Aug 14; 8; 4):e6644. [Full Text/Abstract]
Golden A, Liu J, Cohen-Fix O. Inactivation of the C. elegans lipin homolog leads to ER disorganization and to defects in the breakdown and reassembly of the nuclear envelope. J Cell Sci. 2009 Jun 15; 122 (Pt 12; 122; 2009 Jun 15):1970-8. [Full Text/Abstract]
Arur S, Ohmachi M, Nayak S, Hayes M, Miranda A, Hay A, Golden A, Schedl T. Multiple ERK substrates execute single biological processes in Caenorhabditis elegans germ-line development. Proc Natl Acad Sci U S A. 2009 Mar 24; 106 (106; 2009 Mar 24; 12):4776-81. [Full Text/Abstract]
Edskes HK, McCann LM, Hebert AM, Wickner RB. Prion variants and species barriers among Saccharomyces Ure2 proteins. Genetics. 2009 Mar; 181 (181; 3; 2009 Mar):1159-67. [Full Text/Abstract]
Song MH, Aravind L, Müller-Reichert T, O'Connell KF. The conserved protein SZY-20 opposes the Plk4-related kinase ZYG-1 to limit centrosome size. Dev Cell. 2008 Dec; 15 (2008 Dec; 6; 15):901-12. [Full Text/Abstract]
Song MH, Miliaras NB, Peel N, O'Connell KF. Centrioles: some self-assembly required. Curr Opin Cell Biol. 2008 Dec; 20 (20; 2008 Dec; 6):688-93. [Full Text/Abstract]
Kryndushkin DS, Shewmaker F, Wickner RB. Curing of the [URE3] prion by Btn2p, a Batten disease-related protein. EMBO J. 2008 Oct 22; 27 (20; 2008 Oct 22; 27):2725-35. [Full Text/Abstract]
Wickner RB, Shewmaker F, Kryndushkin D, Edskes HK. Protein inheritance (prions) based on parallel in-register beta-sheet amyloid structures. Bioessays. 2008 Oct; 30 (30; 2008 Oct; 10):955-64. [Full Text/Abstract]
Chattopadhyay MK, Park MH, Tabor H. Hypusine modification for growth is the major function of spermidine in Saccharomyces cerevisiae polyamine auxotrophs grown in limiting spermidine. Proc Natl Acad Sci U S A. 2008 May 6; 105 (105; 2008 May 6; 18):6554-9. [Full Text/Abstract]
Shewmaker F, Ross ED, Tycko R, Wickner RB. Amyloids of shuffled prion domains that form prions have a parallel in-register beta-sheet structure. Biochemistry. 2008 Apr 1; 47 (2008 Apr 1; 13; 47):4000-7. [Full Text/Abstract]
Wickner RB, Dyda F, Tycko R. Amyloid of Rnq1p, the basis of the [PIN+] prion, has a parallel in-register beta-sheet structure. Proc Natl Acad Sci U S A. 2008 Feb 19; 105 (105; 2008 Feb 19; 7):2403-8. [Full Text/Abstract]
Baxa U, Wickner RB, Steven AC, Anderson DE, Marekov LN, Yau WM, Tycko R. Characterization of beta-sheet structure in Ure2p1-89 yeast prion fibrils by solid-state nuclear magnetic resonance. Biochemistry. 2007 Nov 13; 46 (46; 2007 Nov 13; 45):13149-62. [Full Text/Abstract]
Bembenek JN, Richie CT, Squirrell JM, Campbell JM, Eliceiri KW, Poteryaev D, Spang A, Golden A, White JG. Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase. Development. 2007 Nov; 134 (2007 Nov; 134; 21):3837-48. [Full Text/Abstract]
Kemp CA, Song MH, Addepalli MK, Hunter G, O'Connell K. Suppressors of zyg-1 define regulators of centrosome duplication and nuclear association in Caenorhabditis elegans. Genetics. 2007 May; 176 (2007 May; 176; 1):95-113. [Full Text/Abstract]
Golden A, O'Connell KF. Silence is golden: combining RNAi and live cell imaging to study cell cycle regulatory genes during Caenorhabditis elegans development. Methods. 2007 Feb; 41 (2; 41; 2007 Feb):190-7. [Full Text/Abstract]
Golden A, O'Connell KF. Silence is golden: combining RNAi and live cell imaging to study cell cycle regulatory genes during Caenorhabditis elegans development. Methods. 2007 Feb; 41 (2; 41; 2007 Feb):190-7. [Full Text/Abstract]
Stein KK, Davis ES, Hays T, Golden A. Components of the spindle assembly checkpoint regulate the anaphase-promoting complex during meiosis in Caenorhabditis elegans. Genetics. 2007 Jan; 175 (175; 2007 Jan; 1):107-23. [Full Text/Abstract]
Chattopadhyay MK, Tabor CW, Tabor H. Polyamine deficiency leads to accumulation of reactive oxygen species in a spe2Delta mutant of Saccharomyces cerevisiae. Yeast. 2006 Jul 30; 23 (23; 2006 Jul 30; 10):751-61. [Full Text/Abstract]
Hung GC, Masison DC. N-terminal domain of yeast Hsp104 chaperone is dispensable for thermotolerance and prion propagation but necessary for curing prions by Hsp104 overexpression. Genetics. 2006 Jun; 173 (2006 Jun; 2; 173):611-20. [Full Text/Abstract]
Chattopadhyay MK, Tabor CW, Tabor H. Methylthioadenosine and polyamine biosynthesis in a Saccharomyces cerevisiae meu1delta mutant. Biochem Biophys Res Commun. 2006 Apr 28; 343 (343; 2006 Apr 28; 1):203-7. [Full Text/Abstract]
Tutar Y, Song Y, Masison DC. Primate chaperones Hsc70 (constitutive) and Hsp70 (induced) differ functionally in supporting growth and prion propagation in Saccharomyces cerevisiae. Genetics. 2006 Feb; 172 (2006 Feb; 2; 172):851-61. [Full Text/Abstract]
Burrows AE, Sceurman BK, Kosinski ME, Richie CT, Sadler PL, Schumacher JM, Golden A. The C. elegans Myt1 ortholog is required for the proper timing of oocyte maturation. Development. 2006 Feb; 133 (2006 Feb; 133; 4):697-709. [Full Text/Abstract]