U.S. Department of Health and Human Services

Laboratory of Molecular Biology

Michael W. Krause, Ph.D., Chief


A selection of recent and significant publications can be viewed below.

Hwang LC, Vecchiarelli AG, Han YW, Mizuuchi M, Harada Y, Funnell BE, Mizuuchi K. ParA-mediated plasmid partition driven by protein pattern self-organization.EMBO J. 2013 May 2; 32 (2013 May 2; 32; 9) :1238-49. [Full Text/Abstract]
Vecchiarelli AG, Hwang LC, Mizuuchi K. Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism.Proc. Natl. Acad. Sci. U.S.A. 2013 Apr 9; 110 (110; 15; 2013 Apr 9) :E1390-7. [Full Text/Abstract]
Lee JG, Ye Y. Bag6/Bat3/Scythe: a novel chaperone activity with diverse regulatory functions in protein biogenesis and degradation.Bioessays. 2013 Apr; 35 (35; 4; 2013 Apr) :377-85. [Full Text/Abstract]
Lapkouski M, Tian L, Miller JT, Le Grice SF, Yang W. Complexes of HIV-1 RT, NNRTI and RNA/DNA hybrid reveal a structure compatible with RNA degradation.Nat. Struct. Mol. Biol. 2013 Feb; 20 (20; 2; 2013 Feb) :230-6. [Full Text/Abstract]
Xu Y, Cai M, Yang Y, Huang L, Ye Y. SGTA recognizes a noncanonical ubiquitin-like domain in the Bag6-Ubl4A-Trc35 complex to promote endoplasmic reticulum-associated degradation.Cell Rep. 2012 Dec 27; 2 (2012 Dec 27; 6; 2) :1633-44. [Full Text/Abstract]
Vecchiarelli AG, Mizuuchi K, Funnell BE. Surfing biological surfaces: exploiting the nucleoid for partition and transport in bacteria.Mol. Microbiol. 2012 Nov; 86 (86; 2012 Nov; 3) :513-23. [Full Text/Abstract]
Noinaj N, Buchanan SK, Cornelissen CN. The transferrin-iron import system from pathogenic Neisseria species.Mol. Microbiol. 2012 Oct; 86 (86; 2012 Oct; 2) :246-57. [Full Text/Abstract]
Webb CT, Selkrig J, Perry AJ, Noinaj N, Buchanan SK, Lithgow T. Dynamic association of BAM complex modules includes surface exposure of the lipoprotein BamC.J. Mol. Biol. 2012 Sep 28; 422 (422; 2012 Sep 28; 4) :545-55. [Full Text/Abstract]
Nakamura T, Zhao Y, Yamagata Y, Hua YJ, Yang W. Watching DNA polymerase η make a phosphodiester bond.Nature. 2012 Jul 11; 487 (7406; 2012 Jul 11; 487) :196-201. [Full Text/Abstract]
Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK. Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis.Structure. 2012 Jul 3; 20 (20; 2012 Jul 3; 7) :1233-43. [Full Text/Abstract]
Fàbrega A, Rosner JL, Martin RG, Solé M, Vila J. SoxS-dependent coregulation of ompN and ydbK in a multidrug-resistant Escherichia coli strain.FEMS Microbiol. Lett. 2012 Jul; 332 (2012 Jul; 332; 1) :61-7. [Full Text/Abstract]
Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK. Structural engineering of a phage lysin that targets gram-negative pathogens.Proc. Natl. Acad. Sci. U.S.A. 2012 Jun 19; 109 (25; 109; 2012 Jun 19) :9857-62. [Full Text/Abstract]
Zhao Y, Biertümpfel C, Gregory MT, Hua YJ, Hanaoka F, Yang W. Structural basis of human DNA polymerase η-mediated chemoresistance to cisplatin.Proc. Natl. Acad. Sci. U.S.A. 2012 May 8; 109 (109; 19; 2012 May 8) :7269-74. [Full Text/Abstract]
Maggin JE, James JA, Chappie JS, Dyda F, Hickman AB. The amino acid linker between the endonuclease and helicase domains of adeno-associated virus type 5 Rep plays a critical role in DNA-dependent oligomerization.J Virol. 2012 Mar; 86 (86; 6; 2012 Mar) :3337-46. [Full Text/Abstract]
Gowher H, Brick K, Camerini-Otero RD, Felsenfeld G. Vezf1 protein binding sites genome-wide are associated with pausing of elongating RNA polymerase II.Proc. Natl. Acad. Sci. U.S.A. 2012 Feb 14; 109 (109; 7; 2012 Feb 14) :2370-5. [Full Text/Abstract]
Noinaj N, Easley NC, Oke M, Mizuno N, Gumbart J, Boura E, Steere AN, Zak O, Aisen P, Tajkhorshid E, Evans RW, Gorringe AR, Mason AB, Steven AC, Buchanan SK. Structural basis for iron piracy by pathogenic Neisseria.Nature. 2012 Feb 12; 483 (2012 Feb 12; 483; 7387) :53-8. [Full Text/Abstract]
Ramanathan HN, Ye Y. The p97 ATPase associates with EEA1 to regulate the size of early endosomes.Cell Res. 2012 Feb; 22 (2012 Feb; 2; 22) :346-59. [Full Text/Abstract]
Li M, Ivanov V, Mizuuchi M, Mizuuchi K, Craigie R. DNA requirements for assembly and stability of HIV-1 intasomes.Protein Sci. 2012 Feb; 21 (2012 Feb; 2; 21) :249-57. [Full Text/Abstract]
Barnard TJ, Gumbart J, Peterson JH, Noinaj N, Easley NC, Dautin N, Kuszak AJ, Tajkhorshid E, Bernstein HD, Buchanan SK. Molecular basis for the activation of a catalytic asparagine residue in a self-cleaving bacterial autotransporter.J. Mol. Biol. 2012 Jan 6; 415 (2012 Jan 6; 415; 1) :128-42. [Full Text/Abstract]
Gupta S, Gellert M, Yang W. Mechanism of mismatch recognition revealed by human MutSβ bound to unpaired DNA loops.Nat. Struct. Mol. Biol. 2011 Dec 18; 19 (2011 Dec 18; 19; 1) :72-8. [Full Text/Abstract]
Hinnebusch BJ, Jarrett CO, Callison JA, Gardner D, Buchanan SK, Plano GV. Role of the Yersinia pestis Ail protein in preventing a protective polymorphonuclear leukocyte response during bubonic plague.Infect. Immun. 2011 Dec; 79 (79; 12; 2011 Dec) :4984-9. [Full Text/Abstract]
Yamashita S, Lukacik P, Barnard TJ, Noinaj N, Felek S, Tsang TM, Krukonis ES, Hinnebusch BJ, Buchanan SK. Structural insights into Ail-mediated adhesion in Yersinia pestis.Structure. 2011 Nov 9; 19 (19; 2011 Nov 9; 11) :1672-82. [Full Text/Abstract]
Ramanathan HN, Ye Y. Revoking the cellular license to replicate: yet another AAA assignment.Mol. Cell. 2011 Oct 7; 44 (2011 Oct 7; 44; 1) :3-4. [Full Text/Abstract]
He S, Hickman AB, Dyda F, Johnson NP, Chandler M, Ton-Hoang B. Reconstitution of a functional IS608 single-strand transpososome: role of non-canonical base pairing.Nucleic Acids Res. 2011 Oct; 39 (19; 39; 2011 Oct) :8503-12. [Full Text/Abstract]
Chappie JS, Mears JA, Fang S, Leonard M, Schmid SL, Milligan RA, Hinshaw JE, Dyda F. A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke.Cell. 2011 Sep 30; 147 (147; 2011 Sep 30; 1) :209-22. [Full Text/Abstract]
Li X, Wang S, Li Y, Deng C, Steiner LA, Xiao H, Wu C, Bungert J, Gallagher PG, Felsenfeld G, Qiu Y, Huang S. Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes.Blood. 2011 Aug 4; 118 (118; 2011 Aug 4; 5) :1386-94. [Full Text/Abstract]
Fairman JW, Noinaj N, Buchanan SK. The structural biology of β-barrel membrane proteins: a summary of recent reports.Curr. Opin. Struct. Biol. 2011 Aug; 21 (4; 21; 2011 Aug) :523-31. [Full Text/Abstract]
Nery FC, Armata IA, Farley JE, Cho JA, Yaqub U, Chen P, da Hora CC, Wang Q, Tagaya M, Klein C, Tannous B, Caldwell KA, Caldwell GA, Lencer WI, Ye Y, Breakefield XO. TorsinA participates in endoplasmic reticulum-associated degradation.Nat Commun. 2011 Jul 12; 2 (2011 Jul 12; 2) :393. [Full Text/Abstract]
Engel A, Shewmaker F, Edskes HK, Dyda F, Wickner RB. Amyloid of the Candida albicans Ure2p prion domain is infectious and has an in-register parallel β-sheet structure.Biochemistry. 2011 Jul 12; 50 (2011 Jul 12; 50; 27) :5971-8. [Full Text/Abstract]
Wang Q, Liu Y, Soetandyo N, Baek K, Hegde R, Ye Y. A ubiquitin ligase-associated chaperone holdase maintains polypeptides in soluble states for proteasome degradation.Mol. Cell. 2011 Jun 24; 42 (2011 Jun 24; 6; 42) :758-70. [Full Text/Abstract]