U.S. Department of Health and Human Services

Laboratory of Molecular Biology

Susan K. Buchanan, Ph.D., Chief


A selection of recent and significant publications can be viewed below.

Rollauer SE, Sooreshjani MA, Noinaj N, Buchanan SK. Outer membrane protein biogenesis in Gram-negative bacteria. Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5; 370 (1679; 2015 Oct 5; 370). [Full Text/Abstract]
O'Neil PK, Rollauer SE, Noinaj N, Buchanan SK. Fitting the Pieces of the β-Barrel Assembly Machinery Complex. Biochemistry. 2015 Sep 22; 54 (2015 Sep 22; 41; 54):6303-11. [Full Text/Abstract]
Cash DR, Noinaj N, Buchanan SK, Cornelissen CN. Beyond the Crystal Structure: Insight into the Function and Vaccine Potential of TbpA Expressed by Neisseria gonorrhoeae. Infect Immun. 2015 Sep 8; 83 (83; 2015 Sep 8; 11):4438-49. [Full Text/Abstract]
Kuszak AJ, Jacobs D, Gurnev PA, Shiota T, Louis JM, Lithgow T, Bezrukov SM, Rostovtseva TK, Buchanan SK. Evidence of Distinct Channel Conformations and Substrate Binding Affinities for the Mitochondrial Outer Membrane Protein Translocase Pore Tom40. J Biol Chem. 2015 Sep 2; 290 (43; 2015 Sep 2; 290):26204-26217. [Full Text/Abstract]
Gorrepati L, Krause MW, Chen W, Brodigan TM, Correa-Mendez M, Eisenmann DM. Identification of Wnt Pathway Target Genes Regulating the Division and Differentiation of Larval Seam Cells and Vulval Precursor Cells in Caenorhabditis elegans. G3 (Bethesda). 2015 Jun 5; 5 (2015 Jun 5; 8; 5):1551-66. [Full Text/Abstract]
Liu Z, Shi H, Szymczak LC, Aydin T, Yun S, Constas K, Schaeffer A, Ranjan S, Kubba S, Alam E, McMahon DE, He J, Shwartz N, Tian C, Plavskin Y, Lindy A, Dad NA, Sheth S, Amin NM, Zimmerman S, Liu D, Schwarz EM, Smith H, Krause MW, Liu J. Promotion of bone morphogenetic protein signaling by tetraspanins and glycosphingolipids. PLoS Genet. 2015 May; 11 (2015 May; 5; 11):e1005221. [Full Text/Abstract]
Noinaj N, Rollauer SE, Buchanan SK. The β-barrel membrane protein insertase machinery from Gram-negative bacteria. Curr Opin Struct Biol. 2015 Mar 18; 31 (2015 Mar 18; 31):35-42. [Full Text/Abstract]
Shen HH, Leyton DL, Shiota T, Belousoff MJ, Noinaj N, Lu J, Holt SA, Tan K, Selkrig J, Webb CT, Buchanan SK, Martin LL, Lithgow T. Reconstitution of a nanomachine driving the assembly of proteins into bacterial outer membranes. Nat Commun. 2014 Oct 24; 5 (2014 Oct 24; 5):5078. [Full Text/Abstract]
Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G. Scalable and Versatile Genome Editing Using Linear DNAs with Micro-Homology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Sep 23; 198 (2014 Sep 23; 4; 198):1347-56. [Full Text/Abstract]
Noinaj N, Buchanan SK. Structural insights into the transport of small molecules across membranes. Curr Opin Struct Biol. 2014 Mar 26; 27 (2014 Mar 26; 27):8-15. [Full Text/Abstract]
Vecchiarelli AG, Li M, Mizuuchi M, Mizuuchi K. Differential affinities of MinD and MinE to anionic phospholipid influence Min Patterning dynamics in vitro. Mol Microbiol. 2014 Aug; 93 (93; 2014 Aug; 3):453-63. [Full Text/Abstract]
Zhang T, Ye Y. The final moments of misfolded proteins en route to the proteasome. DNA Cell Biol. 2014 Aug; 33 (8; 33; 2014 Aug):477-83. [Full Text/Abstract]
Hickman AB, Ewis HE, Li X, Knapp JA, Laver T, Doss AL, Tolun G, Steven AC, Grishaev A, Bax A, Atkinson PW, Craig NL, Dyda F. Structural basis of hAT transposon end recognition by Hermes, an octameric DNA transposase from Musca domestica. Cell. 2014 Jul 17; 158 (2014 Jul 17; 2; 158):353-367. [Full Text/Abstract]
Noinaj N, Kuszak AJ, Balusek C, Gumbart JC, Buchanan SK. Lateral opening and exit pore formation are required for BamA function. Structure. 2014 Jul 8; 22 (7; 2014 Jul 8; 22):1055-62. [Full Text/Abstract]
Noinaj N, Buchanan SK. FhaC takes a bow to FHA in the two-partner do-si-do. Mol Microbiol. 2014 Jun; 92 (6; 92; 2014 Jun):1155-8. [Full Text/Abstract]
Jackson BM, Abete-Luzi P, Krause MW, Eisenmann DM. Use of an Activated Beta-Catenin to Identify Wnt Pathway Target Genes in Caenorhabditis elegans, Including a Subset of Collagen Genes Expressed in Late Larval Development. G3 (Bethesda). 2014 Apr 16; 4 (2014 Apr 16; 4):733-47. [Full Text/Abstract]
Vecchiarelli AG, Neuman KC, Mizuuchi K. A propagating ATPase gradient drives transport of surface-confined cellular cargo. Proc Natl Acad Sci U S A. 2014 Apr 1; 111 (2014 Apr 1; 111; 13):4880-5. [Full Text/Abstract]
Christianson JC, Ye Y. Cleaning up in the endoplasmic reticulum: ubiquitin in charge. Nat Struct Mol Biol. 2014 Apr; 21 (4; 21; 2014 Apr):325-35. [Full Text/Abstract]
Yan L, Liu W, Zhang H, Liu C, Shang Y, Ye Y, Zhang X, Li W. Ube2g2-gp78-mediated HERP polyubiquitylation is involved in ER stress recovery. J Cell Sci. 2014 Apr 1; 127 (127; Pt 7; 2014 Apr 1):1417-27. [Full Text/Abstract]
Huang CH, Chu YR, Ye Y, Chen X. Role of HERP and a HERP-related protein in HRD1-dependent protein degradation at the endoplasmic reticulum. J Biol Chem. 2014 Feb 14; 289 (2014 Feb 14; 7; 289):4444-54. [Full Text/Abstract]
Lee JG, Kim W, Gygi S, Ye Y. Characterization of the deubiquitinating activity of USP19 and its role in endoplasmic reticulum-associated degradation. J Biol Chem. 2014 Feb 7; 289 (6; 289; 2014 Feb 7):3510-7. [Full Text/Abstract]
Zhuang X, Semenova E, Maric D, Craigie R. Dephosphorylation of barrier-to-autointegration factor by protein phosphatase 4 and its role in cell mitosis. J Biol Chem. 2014 Jan 10; 289 (2014 Jan 10; 2; 289):1119-27. [Full Text/Abstract]
Liu W, Shang Y, Zeng Y, Liu C, Li Y, Zhai L, Wang P, Lou J, Xu P, Ye Y, Li W. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains. EMBO J. 2014 Jan 7; 33 (2014 Jan 7; 33; 1):46-61. [Full Text/Abstract]
Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y. USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. Elife. 2014; 3 (2014; 3):e01369. [Full Text/Abstract]
Felsenfeld G. A brief history of epigenetics. Cold Spring Harb Perspect Biol. 2014 Jan 1; 6 (6; 2014 Jan 1; 1). [Full Text/Abstract]
Ghosh SK, Bond MR, Love DC, Ashwell GG, Krause MW, Hanover JA. Disruption of O-GlcNAc Cycling in C. elegans Perturbs Nucleotide Sugar Pools and Complex Glycans. Front Endocrinol (Lausanne). 2014; 5 (2014; 5):197. [Full Text/Abstract]
Kallampallil J, Wood SJ, O'Dempsey T, Craigie RJ. Nematode infection mimicking paratesticular malignancy. BMJ Case Rep. 2013 (2013). [Full Text/Abstract]
Noinaj N, Cornelissen CN, Buchanan SK. Structural insight into the lactoferrin receptors from pathogenic Neisseria. J Struct Biol. 2013 Oct; 184 (2013 Oct; 184; 1):83-92. [Full Text/Abstract]
Noinaj N, Kuszak AJ, Gumbart JC, Lukacik P, Chang H, Easley NC, Lithgow T, Buchanan SK. Structural insight into the biogenesis of β-barrel membrane proteins. Nature. 2013 Sep 19; 501 (501; 2013 Sep 19; 7467):385-90. [Full Text/Abstract]
Huang CH, Hsiao HT, Chu YR, Ye Y, Chen X. Derlin2 facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum. J Biol Chem. 2013 Aug 30; 288 (35; 288; 2013 Aug 30):25330-9. [Full Text/Abstract]
Chandler M, de la Cruz F, Dyda F, Hickman AB, Moncalian G, Ton-Hoang B. Breaking and joining single-stranded DNA: the HUH endonuclease superfamily. Nat Rev Microbiol. 2013 Aug; 11 (8; 2013 Aug; 11):525-38. [Full Text/Abstract]
Xu Y, Liu Y, Lee JG, Ye Y. A Ubiquitin-like Domain Recruits an Oligomeric Chaperone to a Retrotranslocation Complex in Endoplasmic Reticulum-associated Degradation. J Biol Chem. 2013 Jun 21; 288 (25; 2013 Jun 21; 288):18068-76. [Full Text/Abstract]
Reinke V, Krause M, Okkema P. Transcriptional regulation of gene expression in C. elegans. WormBook. 2013 Jun 4; (2013 Jun 4):1-31. [Full Text/Abstract]
Hwang LC, Vecchiarelli AG, Han YW, Mizuuchi M, Harada Y, Funnell BE, Mizuuchi K. ParA-mediated plasmid partition driven by protein pattern self-organization. EMBO J. 2013 May 2; 32 (2013 May 2; 32; 9):1238-49. [Full Text/Abstract]
Vecchiarelli AG, Hwang LC, Mizuuchi K. Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism. Proc Natl Acad Sci U S A. 2013 Apr 9; 110 (110; 15; 2013 Apr 9):E1390-7. [Full Text/Abstract]
Lee JG, Ye Y. Bag6/Bat3/Scythe: a novel chaperone activity with diverse regulatory functions in protein biogenesis and degradation. Bioessays. 2013 Apr; 35 (35; 4; 2013 Apr):377-85. [Full Text/Abstract]
Lapkouski M, Tian L, Miller JT, Le Grice SF, Yang W. Complexes of HIV-1 RT, NNRTI and RNA/DNA hybrid reveal a structure compatible with RNA degradation. Nat Struct Mol Biol. 2013 Feb; 20 (20; 2; 2013 Feb):230-6. [Full Text/Abstract]
Lukacik P, Barnard TJ, Hinnebusch BJ, Buchanan SK. Specific targeting and killing of Gram-negative pathogens with an engineered phage lytic enzyme. Virulence. 2013 Jan 1; 4 (4; 2013 Jan 1; 1):90-1. [Full Text/Abstract]
Lee JG, Baek K, Soetandyo N, Ye Y. Reversible inactivation of deubiquitinases by reactive oxygen species in vitro and in cells. Nat Commun. 2013; 4 (4; 2013):1568. [Full Text/Abstract]
Xu Y, Cai M, Yang Y, Huang L, Ye Y. SGTA recognizes a noncanonical ubiquitin-like domain in the Bag6-Ubl4A-Trc35 complex to promote endoplasmic reticulum-associated degradation. Cell Rep. 2012 Dec 27; 2 (2012 Dec 27; 6; 2):1633-44. [Full Text/Abstract]
Lukacik P, Barnard TJ, Buchanan SK. Using a bacteriocin structure to engineer a phage lysin that targets Yersinia pestis. Biochem Soc Trans. 2012 Dec 1; 40 (40; 6; 2012 Dec 1):1503-6. [Full Text/Abstract]
Noinaj N, Buchanan SK, Cornelissen CN. The transferrin-iron import system from pathogenic Neisseria species. Mol Microbiol. 2012 Oct; 86 (86; 2012 Oct; 2):246-57. [Full Text/Abstract]
Webb CT, Selkrig J, Perry AJ, Noinaj N, Buchanan SK, Lithgow T. Dynamic association of BAM complex modules includes surface exposure of the lipoprotein BamC. J Mol Biol. 2012 Sep 28; 422 (422; 2012 Sep 28; 4):545-55. [Full Text/Abstract]
Nakamura T, Zhao Y, Yamagata Y, Hua YJ, Yang W. Watching DNA polymerase η make a phosphodiester bond. Nature. 2012 Jul 11; 487 (7406; 2012 Jul 11; 487):196-201. [Full Text/Abstract]
Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK. Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis. Structure. 2012 Jul 3; 20 (20; 2012 Jul 3; 7):1233-43. [Full Text/Abstract]
Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK. Structural engineering of a phage lysin that targets gram-negative pathogens. Proc Natl Acad Sci U S A. 2012 Jun 19; 109 (25; 109; 2012 Jun 19):9857-62. [Full Text/Abstract]
Zhao Y, Biertümpfel C, Gregory MT, Hua YJ, Hanaoka F, Yang W. Structural basis of human DNA polymerase η-mediated chemoresistance to cisplatin. Proc Natl Acad Sci U S A. 2012 May 8; 109 (109; 19; 2012 May 8):7269-74. [Full Text/Abstract]
Gowher H, Brick K, Camerini-Otero RD, Felsenfeld G. Vezf1 protein binding sites genome-wide are associated with pausing of elongating RNA polymerase II. Proc Natl Acad Sci U S A. 2012 Feb 14; 109 (109; 7; 2012 Feb 14):2370-5. [Full Text/Abstract]
Noinaj N, Easley NC, Oke M, Mizuno N, Gumbart J, Boura E, Steere AN, Zak O, Aisen P, Tajkhorshid E, Evans RW, Gorringe AR, Mason AB, Steven AC, Buchanan SK. Structural basis for iron piracy by pathogenic Neisseria. Nature. 2012 Feb 12; 483 (2012 Feb 12; 483; 7387):53-8. [Full Text/Abstract]
Li M, Ivanov V, Mizuuchi M, Mizuuchi K, Craigie R. DNA requirements for assembly and stability of HIV-1 intasomes. Protein Sci. 2012 Feb; 21 (2012 Feb; 2; 21):249-57. [Full Text/Abstract]