U.S. Department of Health and Human Services

Laboratory of Molecular Biology

Susan K. Buchanan, Ph.D., Chief


A selection of recent and significant publications can be viewed below.

Dhalla AK, Yang M, Ning Y, Kahlig KM, Krause M, Rajamani S, Belardinelli L. Blockade of Na+ Channels in Pancreatic α-Cells Has Antidiabetic Effects. Diabetes. 2014 May 8; 63 (63; 10; 2014 May 8):3545-56. [Full Text/Abstract]
Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G. Scalable and Versatile Genome Editing Using Linear DNAs with Micro-Homology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Sep 23; 198 (2014 Sep 23; 4; 198):1347-56. [Full Text/Abstract]
Noinaj N, Buchanan SK. Structural insights into the transport of small molecules across membranes. Curr Opin Struct Biol. 2014 Mar 26; 27 (2014 Mar 26; 27):8-15. [Full Text/Abstract]
Vecchiarelli AG, Li M, Mizuuchi M, Mizuuchi K. Differential affinities of MinD and MinE to anionic phospholipid influence Min Patterning dynamics in vitro. Mol Microbiol. 2014 Aug; 93 (93; 2014 Aug; 3):453-63. [Full Text/Abstract]
Zhang T, Ye Y. The final moments of misfolded proteins en route to the proteasome. DNA Cell Biol. 2014 Aug; 33 (8; 33; 2014 Aug):477-83. [Full Text/Abstract]
Hickman AB, Ewis HE, Li X, Knapp JA, Laver T, Doss AL, Tolun G, Steven AC, Grishaev A, Bax A, Atkinson PW, Craig NL, Dyda F. Structural basis of hAT transposon end recognition by Hermes, an octameric DNA transposase from Musca domestica. Cell. 2014 Jul 17; 158 (2014 Jul 17; 2; 158):353-367. [Full Text/Abstract]
Noinaj N, Kuszak AJ, Balusek C, Gumbart JC, Buchanan SK. Lateral opening and exit pore formation are required for BamA function. Structure. 2014 Jul 8; 22 (7; 2014 Jul 8; 22):1055-62. [Full Text/Abstract]
Noinaj N, Buchanan SK. FhaC takes a bow to FHA in the two-partner do-si-do. Mol Microbiol. 2014 Jun; 92 (6; 92; 2014 Jun):1155-8. [Full Text/Abstract]
Jackson BM, Abete-Luzi P, Krause MW, Eisenmann DM. Use of an Activated Beta-Catenin to Identify Wnt Pathway Target Genes in Caenorhabditis elegans, Including a Subset of Collagen Genes Expressed in Late Larval Development. G3 (Bethesda). 2014 Apr 16; 4 (2014 Apr 16; 4):733-47. [Full Text/Abstract]
Christianson JC, Ye Y. Cleaning up in the endoplasmic reticulum: ubiquitin in charge. Nat Struct Mol Biol. 2014 Apr; 21 (4; 21; 2014 Apr):325-35. [Full Text/Abstract]
Yan L, Liu W, Zhang H, Liu C, Shang Y, Ye Y, Zhang X, Li W. Ube2g2-gp78-mediated HERP polyubiquitylation is involved in ER stress recovery. J Cell Sci. 2014 Apr 1; 127 (127; Pt 7; 2014 Apr 1):1417-27. [Full Text/Abstract]
Vecchiarelli AG, Neuman KC, Mizuuchi K. A propagating ATPase gradient drives transport of surface-confined cellular cargo. Proc Natl Acad Sci U S A. 2014 Apr 1; 111 (2014 Apr 1; 111; 13):4880-5. [Full Text/Abstract]
Huang CH, Chu YR, Ye Y, Chen X. Role of HERP and a HERP-related protein in HRD1-dependent protein degradation at the endoplasmic reticulum. J Biol Chem. 2014 Feb 14; 289 (2014 Feb 14; 7; 289):4444-54. [Full Text/Abstract]
Lee JG, Kim W, Gygi S, Ye Y. Characterization of the deubiquitinating activity of USP19 and its role in endoplasmic reticulum-associated degradation. J Biol Chem. 2014 Feb 7; 289 (6; 289; 2014 Feb 7):3510-7. [Full Text/Abstract]
Zhuang X, Semenova E, Maric D, Craigie R. Dephosphorylation of barrier-to-autointegration factor by protein phosphatase 4 and its role in cell mitosis. J Biol Chem. 2014 Jan 10; 289 (2014 Jan 10; 2; 289):1119-27. [Full Text/Abstract]
Liu W, Shang Y, Zeng Y, Liu C, Li Y, Zhai L, Wang P, Lou J, Xu P, Ye Y, Li W. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains. EMBO J. 2014 Jan 7; 33 (2014 Jan 7; 33; 1):46-61. [Full Text/Abstract]
Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y. USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. Elife. 2014; 3 (2014; 3):e01369. [Full Text/Abstract]
Felsenfeld G. A brief history of epigenetics. Cold Spring Harb Perspect Biol. 2014 Jan 1; 6 (6; 2014 Jan 1; 1). [Full Text/Abstract]
Kallampallil J, Wood SJ, O'Dempsey T, Craigie RJ. Nematode infection mimicking paratesticular malignancy. BMJ Case Rep. 2013 (2013). [Full Text/Abstract]
Sethi S, Pothineni NK, Syal G, Ali SM, Krause MW. Aortic intimal sarcoma masquerading as bilateral renal artery stenosis. J Nephrol. 2013 Sep-Oct; 26 (2013 Sep-Oct; 5; 26):941-4. [Full Text/Abstract]
Huang CH, Hsiao HT, Chu YR, Ye Y, Chen X. Derlin2 facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum. J Biol Chem. 2013 Aug 30; 288 (35; 288; 2013 Aug 30):25330-9. [Full Text/Abstract]
Chandler M, de la Cruz F, Dyda F, Hickman AB, Moncalian G, Ton-Hoang B. Breaking and joining single-stranded DNA: the HUH endonuclease superfamily. Nat Rev Microbiol. 2013 Aug; 11 (8; 2013 Aug; 11):525-38. [Full Text/Abstract]
Xu Y, Liu Y, Lee JG, Ye Y. A Ubiquitin-like Domain Recruits an Oligomeric Chaperone to a Retrotranslocation Complex in Endoplasmic Reticulum-associated Degradation. J Biol Chem. 2013 Jun 21; 288 (25; 2013 Jun 21; 288):18068-76. [Full Text/Abstract]
Reinke V, Krause M, Okkema P. Transcriptional regulation of gene expression in C. elegans. WormBook. 2013 Jun 4; (2013 Jun 4):1-31. [Full Text/Abstract]
Hwang LC, Vecchiarelli AG, Han YW, Mizuuchi M, Harada Y, Funnell BE, Mizuuchi K. ParA-mediated plasmid partition driven by protein pattern self-organization. EMBO J. 2013 May 2; 32 (2013 May 2; 32; 9):1238-49. [Full Text/Abstract]
Vecchiarelli AG, Hwang LC, Mizuuchi K. Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism. Proc Natl Acad Sci U S A. 2013 Apr 9; 110 (110; 15; 2013 Apr 9):E1390-7. [Full Text/Abstract]
Lee JG, Ye Y. Bag6/Bat3/Scythe: a novel chaperone activity with diverse regulatory functions in protein biogenesis and degradation. Bioessays. 2013 Apr; 35 (35; 4; 2013 Apr):377-85. [Full Text/Abstract]
Lapkouski M, Tian L, Miller JT, Le Grice SF, Yang W. Complexes of HIV-1 RT, NNRTI and RNA/DNA hybrid reveal a structure compatible with RNA degradation. Nat. Struct. Mol. Biol. 2013 Feb; 20 (20; 2; 2013 Feb):230-6. [Full Text/Abstract]
Lee JG, Baek K, Soetandyo N, Ye Y. Reversible inactivation of deubiquitinases by reactive oxygen species in vitro and in cells. Nat Commun. 2013; 4 (4; 2013):1568. [Full Text/Abstract]
Xu Y, Cai M, Yang Y, Huang L, Ye Y. SGTA recognizes a noncanonical ubiquitin-like domain in the Bag6-Ubl4A-Trc35 complex to promote endoplasmic reticulum-associated degradation. Cell Rep. 2012 Dec 27; 2 (2012 Dec 27; 6; 2):1633-44. [Full Text/Abstract]
Vecchiarelli AG, Mizuuchi K, Funnell BE. Surfing biological surfaces: exploiting the nucleoid for partition and transport in bacteria. Mol Microbiol. 2012 Nov; 86 (86; 2012 Nov; 3):513-23. [Full Text/Abstract]
Noinaj N, Buchanan SK, Cornelissen CN. The transferrin-iron import system from pathogenic Neisseria species. Mol Microbiol. 2012 Oct; 86 (86; 2012 Oct; 2):246-57. [Full Text/Abstract]
Webb CT, Selkrig J, Perry AJ, Noinaj N, Buchanan SK, Lithgow T. Dynamic association of BAM complex modules includes surface exposure of the lipoprotein BamC. J Mol Biol. 2012 Sep 28; 422 (422; 2012 Sep 28; 4):545-55. [Full Text/Abstract]
Liu Y, Ye Y. Roles of p97-associated deubiquitinases in protein quality control at the endoplasmic reticulum. Curr Protein Pept Sci. 2012 Aug; 13 (2012 Aug; 13; 5):436-46. [Full Text/Abstract]
Nakamura T, Zhao Y, Yamagata Y, Hua YJ, Yang W. Watching DNA polymerase η make a phosphodiester bond. Nature. 2012 Jul 11; 487 (7406; 2012 Jul 11; 487):196-201. [Full Text/Abstract]
Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK. Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis. Structure. 2012 Jul 3; 20 (20; 2012 Jul 3; 7):1233-43. [Full Text/Abstract]
Fàbrega A, Rosner JL, Martin RG, Solé M, Vila J. SoxS-dependent coregulation of ompN and ydbK in a multidrug-resistant Escherichia coli strain. FEMS Microbiol Lett. 2012 Jul; 332 (2012 Jul; 332; 1):61-7. [Full Text/Abstract]
Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK. Structural engineering of a phage lysin that targets gram-negative pathogens. Proc Natl Acad Sci U S A. 2012 Jun 19; 109 (25; 109; 2012 Jun 19):9857-62. [Full Text/Abstract]
Zhao Y, Biertümpfel C, Gregory MT, Hua YJ, Hanaoka F, Yang W. Structural basis of human DNA polymerase η-mediated chemoresistance to cisplatin. Proc. Natl. Acad. Sci. U.S.A. 2012 May 8; 109 (109; 19; 2012 May 8):7269-74. [Full Text/Abstract]
Maggin JE, James JA, Chappie JS, Dyda F, Hickman AB. The amino acid linker between the endonuclease and helicase domains of adeno-associated virus type 5 Rep plays a critical role in DNA-dependent oligomerization. J Virol. 2012 Mar; 86 (86; 6; 2012 Mar):3337-46. [Full Text/Abstract]
Gowher H, Brick K, Camerini-Otero RD, Felsenfeld G. Vezf1 protein binding sites genome-wide are associated with pausing of elongating RNA polymerase II. Proc Natl Acad Sci U S A. 2012 Feb 14; 109 (109; 7; 2012 Feb 14):2370-5. [Full Text/Abstract]
Noinaj N, Easley NC, Oke M, Mizuno N, Gumbart J, Boura E, Steere AN, Zak O, Aisen P, Tajkhorshid E, Evans RW, Gorringe AR, Mason AB, Steven AC, Buchanan SK. Structural basis for iron piracy by pathogenic Neisseria. Nature. 2012 Feb 12; 483 (2012 Feb 12; 483; 7387):53-8. [Full Text/Abstract]
Li M, Ivanov V, Mizuuchi M, Mizuuchi K, Craigie R. DNA requirements for assembly and stability of HIV-1 intasomes. Protein Sci. 2012 Feb; 21 (2012 Feb; 2; 21):249-57. [Full Text/Abstract]
Ramanathan HN, Ye Y. The p97 ATPase associates with EEA1 to regulate the size of early endosomes. Cell Res. 2012 Feb; 22 (2012 Feb; 2; 22):346-59. [Full Text/Abstract]
Barnard TJ, Gumbart J, Peterson JH, Noinaj N, Easley NC, Dautin N, Kuszak AJ, Tajkhorshid E, Bernstein HD, Buchanan SK. Molecular basis for the activation of a catalytic asparagine residue in a self-cleaving bacterial autotransporter. J Mol Biol. 2012 Jan 6; 415 (2012 Jan 6; 415; 1):128-42. [Full Text/Abstract]
Ramanathan HN, Ye Y. Cellular strategies for making monoubiquitin signals. Crit Rev Biochem Mol Biol. 2012 Jan-Feb; 47 (2012 Jan-Feb; 1; 47):17-28. [Full Text/Abstract]
Gupta S, Gellert M, Yang W. Mechanism of mismatch recognition revealed by human MutSβ bound to unpaired DNA loops. Nat. Struct. Mol. Biol. 2011 Dec 18; 19 (2011 Dec 18; 19; 1):72-8. [Full Text/Abstract]
Hinnebusch BJ, Jarrett CO, Callison JA, Gardner D, Buchanan SK, Plano GV. Role of the Yersinia pestis Ail protein in preventing a protective polymorphonuclear leukocyte response during bubonic plague. Infect Immun. 2011 Dec; 79 (79; 12; 2011 Dec):4984-9. [Full Text/Abstract]
Yamashita S, Lukacik P, Barnard TJ, Noinaj N, Felek S, Tsang TM, Krukonis ES, Hinnebusch BJ, Buchanan SK. Structural insights into Ail-mediated adhesion in Yersinia pestis. Structure. 2011 Nov 9; 19 (19; 2011 Nov 9; 11):1672-82. [Full Text/Abstract]
Ramanathan HN, Ye Y. Revoking the cellular license to replicate: yet another AAA assignment. Mol Cell. 2011 Oct 7; 44 (2011 Oct 7; 44; 1):3-4. [Full Text/Abstract]