U.S. Department of Health and Human Services

NIDDK Technology Highlights

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5′-HS4 chicken β-globin insulator (DNA sequence acting as an insulator for the effects of cis-acting regulatory elements)    
Gary Felsenfeld, Ph.D.
A newly characterized chromatin insulator element isolated from the DNA of a higher eukaryotic organism ​and contained in vector constructs is described... Additional Details​


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Stat5a LoxP/Stat5b LoxP (Stat5a/Stat5b tm2Mam)
Lothar Hennighausen, Ph.D.
Conditional knockout of Stat5a and Stat5b: Combined deletion of conserved Stat5a and Stat5b in mammary
epithelium at different times during pregnancy reveal multiple distinct functions... Additional Details

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Sphk1 KO
A Sphingosine kinase 1 (Sphk1) knockout mouse… Additional Details


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M2 Muscarinic receptor KO (Chrm2 tm1Jwe) 
M2 Muscarinic Receptor Knockout: role in learning and memory, and mediating analgesic effectsof muscarinic agonists... Additional Details


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Smad4 KO (Smad4 tm1Cxd)
Smad4 knockout: Smad 4 is essential for epiblast proliferation and mesoderm induction in early embryogenesis… Additional Details


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Inducible podocyte-specific gene expression in transgenic mice (Podocin/rtTA DNA transgene)
The podocyte plays a key role in glomerular function and glomerular disease. To facilitate studies of podocyte function, we have developed a transgenic mouse model with inducible expression in the podocyte… Additional Details


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​UTX LoxP   
UTX-flox.  Conditional knockout mice for the histone demethylase UTX (Kdm6a) conditional knockout will help understand its role as a tumor suppressor… Additional Details


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Brca1 LoxP   
BRCA1 conditional knockouts.  BRCA1 LoxP mutations allow selective inactivation of the gene in mammary epithelial cells (accelerating breast tumor formation) and in cardiomyocytes (impairing cardiac remodeling that may increase the risk of heart failure following myocardial infarction)… Additional Details​​​​​​​ ​​​​