U.S. Department of Health and Human Services

 Contact Info

Tel: 301-402-2616
Email: piknovab@mail.nih.gov

 Select Experience

  • NIH Staff ScientistPresent Molecular Medicine Branch, NIDDK2010
  • NIH Contractor Staff ScientistMolecular Medicine Branch, NIDDK2005–2010
  • Senior Research FellowNational Biomedical Electron Paramagnetic Resonance Center, Medical College of Wisconsin2003–2005
  • Postdoctoral FellowMolecular Medicine Department, Oregon Health & Science University1998–2003
  • Postdoctoral FellowBiochemistry Department, University of Illinois1996–1998
  • Postdoctoral FellowBiochemistry Department, University of Virginia1993–1996
  • Pre- and Postdoctoral FellowNational Centre for Scientific Research, Toulouse, France1991–1993
  • Ph.D.Comenius University, Bratislava, Slovakia1992

 Related Links


Barbora Piknova, Ph.D.

Staff Scientist, Molecular Biology and Genetics SectionMolecular Medicine Branch
  • Biomedical Engineering/Biophysics/Physics


A selection of recent and significant publications can be viewed below.

Piknova B, Park JW, Cassel KS, Gilliard CN, Schechter AN. Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method. J Vis Exp. 2016 Dec 25; (118; 2016 Dec 25). [Full Text/Abstract]
Piknova B, Schechter AN. Acid Test for Nitrite Pharmacology. Hypertension. 2016 Oct 31; 69 (69; 2016 Oct 31; 1):13-14. [Full Text/Abstract]
Piknova B, Park JW, Kwan Jeff Lam K, Schechter AN. Nitrate as a source of nitrite and nitric oxide during exercise hyperemia in rat skeletal muscle. Nitric Oxide. 2016 Mar 18; 55-56 (2016 Mar 18; 55-56):54-61. [Full Text/Abstract]
Monteiro T, Rodrigues PR, Gonçalves AL, Moura JJ, Jubete E, Añorga L, Piknova B, Schechter AN, Silveira CM, Almeida MG. Construction of effective disposable biosensors for point of care testing of nitrite. Talanta. 2015 Sep 1; 142 (142; 2015 Sep 1):246-51. [Full Text/Abstract]
Piknova B, Park JW, Swanson KM, Dey S, Noguchi CT, Schechter AN. Skeletal muscle as an endogenous nitrate reservoir. Nitric Oxide. 2015 Feb 26; 47 (2015 Feb 26; 47):10-16. [Full Text/Abstract]
Park JW, Piknova B, Nghiem K, Lozier JN, Schechter AN. Inhibitory effect of nitrite on coagulation processes demonstrated by thrombelastography. Nitric Oxide. 2014 Aug 31; 40 (40; 2014 Aug 31):45-51. [Full Text/Abstract]
Akrawinthawong K, Park JW, Piknova B, Sibmooh N, Fucharoen S, Schechter AN. A flow cytometric analysis of the inhibition of platelet reactivity due to nitrite reduction by deoxygenated erythrocytes. PLoS One. 2014 Mar 18; 9 (9; 3; 2014 Mar 18):e92435. [Full Text/Abstract]
Wang D, Piknova B, Solomon SB, Cortes-Puch I, Kern SJ, Sun J, Kanias T, Gladwin MT, Helms C, Kim-Shapiro DB, Schechter AN, Natanson C. In vivo reduction of cell-free methemoglobin to oxyhemoglobin results in vasoconstriction in canines. Transfusion. 2013 Dec; 53 (2013 Dec; 12; 53):3149-63. [Full Text/Abstract]
Piknova B, Schechter AN. Comments on 'vascular effects of dietary nitrate (as found in green leafy vegetables and beetroot) via the nitrate-nitrite-nitric oxide pathway'. Br J Clin Pharmacol. 2013 Jun; 75 (6; 75; 2013 Jun):1541-2. [Full Text/Abstract]
Park JW, Piknova B, Kurtz J, Seetharaman S, Wagner SJ, Schechter AN. Effect of storage on levels of nitric oxide metabolites in platelet preparations. Transfusion. 2013 Mar; 53 (2013 Mar; 3; 53):637-44. [Full Text/Abstract]
Park JW, Piknova B, Schechter AN. In reply. Transfusion. 2013 Jan; 53 (53; 1; 2013 Jan):235. [Full Text/Abstract]
Park JW, Piknova B, Huang PL, Noguchi CT, Schechter AN. Effect of blood nitrite and nitrate levels on murine platelet function. PLoS One. 2013; 8 (8; 2; 2013):e55699. [Full Text/Abstract]
Solomon SB, Bellavia L, Sweeney D, Piknova B, Perlegas A, Helms CC, Ferreyra GA, Bruce King S, Raat NJ, Kern SJ, Sun J, McPhail LC, Schechter AN, Natanson C, Gladwin MT, Kim-Shapiro DB. Angeli's salt counteracts the vasoactive effects of elevated plasma hemoglobin. Free Radic Biol Med. 2012 Dec 15; 53 (2012 Dec 15; 12; 53):2229-39. [Full Text/Abstract]
Alnaeeli M, Wang L, Piknova B, Rogers H, Li X, Noguchi CT. Erythropoietin in brain development and beyond. Anat Res Int. 2012; 2012 (2012):953264. [Full Text/Abstract]
Srihirun S, Sriwantana T, Unchern S, Kittikool D, Noulsri E, Pattanapanyasat K, Fucharoen S, Piknova B, Schechter AN, Sibmooh N. Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation. PLoS One. 2012; 7 (2012; 7; 1):e30380. [Full Text/Abstract]
Qazi MA, Rizzatti F, Piknova B, Sibmooh N, Stroncek DF, Schechter AN. Effect of storage levels of nitric oxide derivatives in blood components. F1000Res. 2012 Oct 22; 1 (2012 Oct 22; 1):35. [Full Text/Abstract]
Piknova B, Kocharyan A, Schechter AN, Silva AC. The role of nitrite in neurovascular coupling. Brain Res. 2011 Aug 17; 1407 (1407; 2011 Aug 17):62-8. [Full Text/Abstract]
Hanson MS, Piknova B, Keszler A, Diers AR, Wang X, Gladwin MT, Hillery CA, Hogg N. Methaemalbumin formation in sickle cell disease: effect on oxidative protein modification and HO-1 induction. Br J Haematol. 2011 Aug; 154 (4; 2011 Aug; 154):502-11. [Full Text/Abstract]
Teng R, Calvert JW, Sibmooh N, Piknova B, Suzuki N, Sun J, Martinez K, Yamamoto M, Schechter AN, Lefer DJ, Noguchi CT. Acute erythropoietin cardioprotection is mediated by endothelial response. Basic Res Cardiol. 2011 May; 106 (106; 3; 2011 May):343-54. [Full Text/Abstract]
Beleslin-Čokić BB, Cokić VP, Wang L, Piknova B, Teng R, Schechter AN, Noguchi CT. Erythropoietin and hypoxia increase erythropoietin receptor and nitric oxide levels in lung microvascular endothelial cells. Cytokine. 2011 May; 54 (2; 54; 2011 May):129-35. [Full Text/Abstract]
Piknova B, Schechter AN. Measurement of nitrite in blood samples using the ferricyanide-based hemoglobin oxidation assay. Methods Mol Biol. 2011; 704 (704; 2011):39-56. [Full Text/Abstract]
Piknova B, Keszler A, Hogg N, Schechter AN. The reaction of cell-free oxyhemoglobin with nitrite under physiologically relevant conditions: Implications for nitrite-based therapies. Nitric Oxide. 2009 Mar; 20 (20; 2; 2009 Mar):88-94. [Full Text/Abstract]
Keszler A, Piknova B, Schechter AN, Hogg N. The reaction between nitrite and oxyhemoglobin: a mechanistic study. J Biol Chem. 2008 Apr 11; 283 (283; 15; 2008 Apr 11):9615-22. [Full Text/Abstract]
Sibmooh N, Piknova B, Rizzatti F, Schechter AN. Oxidation of iron-nitrosyl-hemoglobin by dehydroascorbic acid releases nitric oxide to form nitrite in human erythrocytes. Biochemistry. 2008 Mar 4; 47 (2008 Mar 4; 9; 47):2989-96. [Full Text/Abstract]
Chen K, Piknova B, Pittman RN, Schechter AN, Popel AS. Nitric oxide from nitrite reduction by hemoglobin in the plasma and erythrocytes. Nitric Oxide. 2008 Feb; 18 (2008 Feb; 1; 18):47-60. [Full Text/Abstract]
Piknova B, Gladwin MT, Schechter AN, Hogg N. Electron paramagnetic resonance analysis of nitrosylhemoglobin in humans during NO inhalation. J Biol Chem. 2005 Dec 9; 280 (280; 2005 Dec 9; 49):40583-8. [Full Text/Abstract]
Yan W, Piknova B, Hall SB. The collapse of monolayers containing pulmonary surfactant phospholipids is kinetically determined. Biophys J. 2005 Jul; 89 (2005 Jul; 1; 89):306-14. [Full Text/Abstract]
Crawford JH, Chacko BK, Pruitt HM, Piknova B, Hogg N, Patel RP. Transduction of NO-bioactivity by the red blood cell in sepsis: novel mechanisms of vasodilation during acute inflammatory disease. Blood. 2004 Sep 1; 104 (2004 Sep 1; 104; 5):1375-82. [Full Text/Abstract]
Piknova B, Schram V, Hall SB. Pulmonary surfactant: phase behavior and function. Curr Opin Struct Biol. 2002 Aug; 12 (4; 12; 2002 Aug):487-94. [Full Text/Abstract]
Piknova B, Schief WR, Vogel V, Discher BM, Hall SB. Discrepancy between phase behavior of lung surfactant phospholipids and the classical model of surfactant function. Biophys J. 2001 Oct; 81 (81; 4; 2001 Oct):2172-80. [Full Text/Abstract]
Piknová B, Marsh D, Thompson TE. Fluorescence quenching and electron spin resonance study of percolation in a two-phase lipid bilayer containing bacteriorhodopsin. Biophys J. 1997 Jun; 72 (72; 1997 Jun; 6):2660-8. [Full Text/Abstract]
Piknová B, Marsh D, Thompson TE. Fluorescence-quenching study of percolation and compartmentalization in two-phase lipid bilayers. Biophys J. 1996 Aug; 71 (71; 2; 1996 Aug):892-7. [Full Text/Abstract]
Hianik T, Buckin VA, Piknová B. Can a single bacteriorhodopsin molecule change the structural state of one liposome?. Gen Physiol Biophys. 1994 Dec; 13 (6; 13; 1994 Dec):493-501. [Full Text/Abstract]
Tocanne JF, Cézanne L, Lopez A, Piknova B, Schram V, Tournier JF, Welby M. Lipid domains and lipid/protein interactions in biological membranes. Chem Phys Lipids. 1994 Sep 6; 73 (1994 Sep 6; 1-2; 73):139-58. [Full Text/Abstract]
Piknová B, Pérochon E, Tocanne JF. Hydrophobic mismatch and long-range protein/lipid interactions in bacteriorhodopsin/phosphatidylcholine vesicles. Eur J Biochem. 1993 Dec 1; 218 (2; 1993 Dec 1; 218):385-96. [Full Text/Abstract]
Piknová B, Hianik T, Shestimirov VN, Shnyrov VL. Thermodynamical characteristics and volume compressibility of dipalmitoylphosphatidylcholine liposomes containing bacteriorhodopsin. Gen Physiol Biophys. 1991 Aug; 10 (1991 Aug; 4; 10):395-409. [Full Text/Abstract]