Our research examines the role of nitric oxide in vascular homeostasis, especially in neurovascular coupling and hemodynamic response in the brain. We focus on nitrite as an alternative source of nitric oxide and in the possibility of involvement of ascorbic acid in nitrite conversion to nitric oxide, especially in the brain tissue. Other areas of research include nitric oxide metabolism and the balance of NOx compounds (nitrite, nitrate, and RSNO) in the vasculature and tissues that are associated with limited oxygen supply during ischemia. We examine the possible use of NOx compounds in the prevention and treatment of reperfusion injury. Recently, our focus shifted to mammalian tissue nitrate and nitrite reductases as well as nonenzymatic nitrite reduction into nitric oxide and their importance in normal physiology and as a possible source of nitric oxide for exercise-induced hyperemia.