Our work supports clinicians and scientists who need to conduct chemical analysis based mainly on Mass Spectrometry (MS). We have conducted chemical analysis of small molecules in clinical or biological samples. Chromatography is a tool used to separate individual compounds that then are sent to a detector. Our detector of choice is MS, which is very powerful in analyzing masses of molecules in a sample. MS is a technique that studies masses of molecular ions or ionic fragments, and we use them like pieces of a puzzle to identify the whole structure of the molecule. This combination of techniques, chromatography and MS, therefore, helps determine the sample’s molecular components. It reveals the chemical structures of molecules in a sample and it can provide quantitative values. Although sometimes the amounts of chemical compounds in a sample are neglected, numbers are vital in a clinical environment.