U.S. Department of Health and Human Services
Harris Bernstein

 Contact Info

Tel: 301-402-4770
Email: harris_bernstein@nih.gov

 Select Experience

  • Ph.D.Massachusetts Institute of Technology1987
  • B.A.Harvard University1980

 Related Links


Harris D. Bernstein, Ph.D.

Section Chief, Protein Biogenesis SectionGenetics and Biochemistry Branch
  • Microbiology/Infectious diseases (non-viral)
  • Molecular Biology/Biochemistry


A selection of recent and significant publications can be viewed below.

Bernstein HD. Looks can be deceiving: recent insights into the mechanism of protein secretion by the autotransporter pathway. Mol Microbiol. 2015 Apr 17; 97 (2015 Apr 17; 2; 97):205-15. [Full Text/Abstract]
Wilson MM, Anderson DE, Bernstein HD. Analysis of the outer membrane proteome and secretome of Bacteroides fragilis reveals a multiplicity of secretion mechanisms. PLoS One. 2015 Feb 6; 10 (2; 10; 2015 Feb 6):e0117732. [Full Text/Abstract]
Roman-Hernandez G, Peterson JH, Bernstein HD. Reconstitution of bacterial autotransporter assembly using purified components. Elife. 2014 Sep 2; 3 (2014 Sep 2; 3). [Full Text/Abstract]
Kang'ethe W, Bernstein HD. Charge-dependent secretion of an intrinsically disordered protein via the autotransporter pathway. Proc Natl Acad Sci U S A. 2013 Nov 5; 110 (110; 2013 Nov 5; 45):E4246-55. [Full Text/Abstract]
Pavlova O, Peterson JH, Ieva R, Bernstein HD. Mechanistic link between β barrel assembly and the initiation of autotransporter secretion. Proc Natl Acad Sci U S A. 2013 Mar 5; 110 (110; 10; 2013 Mar 5):E938-47. [Full Text/Abstract]
Bernstein HD. Cell biology: All clear for ribosome landing. Nature. 2012 Dec 13; 492 (492; 2012 Dec 13; 7428):189-91. [Full Text/Abstract]
Ieva R, Tian P, Peterson JH, Bernstein HD. Sequential and spatially restricted interactions of assembly factors with an autotransporter beta domain. Proc Natl Acad Sci U S A. 2011 Aug 2; 108 (108; 2011 Aug 2; 31):E383-91. [Full Text/Abstract]
Peterson JH, Tian P, Ieva R, Dautin N, Bernstein HD. Secretion of a bacterial virulence factor is driven by the folding of a C-terminal segment. Proc Natl Acad Sci U S A. 2010 Oct 12; 107 (107; 2010 Oct 12; 41):17739-44. [Full Text/Abstract]
Peterson JH, Woolhead CA, Bernstein HD. The conformation of a nascent polypeptide inside the ribosome tunnel affects protein targeting and protein folding. Mol Microbiol. 2010 Oct; 78 (2010 Oct; 78; 1):203-17. [Full Text/Abstract]
Ieva R, Bernstein HD. Interaction of an autotransporter passenger domain with BamA during its translocation across the bacterial outer membrane. Proc Natl Acad Sci U S A. 2009 Nov 10; 106 (106; 2009 Nov 10; 45):19120-5. [Full Text/Abstract]
Yap MN, Bernstein HD. The plasticity of a translation arrest motif yields insights into nascent polypeptide recognition inside the ribosome tunnel. Mol Cell. 2009 Apr 24; 34 (2; 2009 Apr 24; 34):201-11. [Full Text/Abstract]
Ieva R, Skillman KM, Bernstein HD. Incorporation of a polypeptide segment into the beta-domain pore during the assembly of a bacterial autotransporter. Mol Microbiol. 2008 Jan; 67 (2008 Jan; 67; 1):188-201. [Full Text/Abstract]
Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK. Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Nat Struct Mol Biol. 2007 Dec; 14 (14; 2007 Dec; 12):1214-20. [Full Text/Abstract]
Dautin N, Barnard TJ, Anderson DE, Bernstein HD. Cleavage of a bacterial autotransporter by an evolutionarily convergent autocatalytic mechanism. EMBO J. 2007 Apr 4; 26 (2007 Apr 4; 7; 26):1942-52. [Full Text/Abstract]
Dautin N, Bernstein HD. Protein secretion in gram-negative bacteria via the autotransporter pathway. Annu Rev Microbiol. 2007; 61 (61; 2007):89-112. [Full Text/Abstract]