U.S. Department of Health and Human Services

 Contact Info

Tel: 301-594-3122
Email: johnl@niddk.nih.gov

 Select Experience

  • HeadProtein Engineering and Chemistry Group, Laboratory of Chemical Physics, NIDDK, NIH 2005-present
  • Research Biologist, Laboratory of Chemical Physics, NIDDK, NIH 1996-2005
  • Staff FellowLaboratory of Cellular and Developmental Biology, NIDDK, NIH 1990-1996
  • FellowDivision of Cancer Biology and Diagnosis, NCI, NIH 1986-1989

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    John Louis Medabalimi, Ph.D. (alias: John M. Louis)

    Research Biologist, Office of the Chief, Laboratory of Chemical Physics
    • Chemistry/Chemical Biology
    • Molecular Biology/Biochemistry


    A selection of recent and significant publications can be viewed below.

    Roche J, Louis JM, Grishaev A, Ying J, Bax A. Dissociation of the trimeric gp41 ectodomain at the lipid-water interface suggests an active role in HIV-1 Env-mediated membrane fusion. Proc Natl Acad Sci U S A. 2014 Mar 4; 111 (2014 Mar 4; 111; 9):3425-30. [Full Text/Abstract]
    Sayer JM, Aniana A, Louis JM. Mechanism of dissociative inhibition of HIV protease and its autoprocessing from a precursor. J Mol Biol. 2012 Sep 14; 422 (2012 Sep 14; 422; 2):230-44. [Full Text/Abstract]
    Chung HS, McHale K, Louis JM, Eaton WA. Single-molecule fluorescence experiments determine protein folding transition path times. Science. 2012 Feb 24; 335 (335; 6071; 2012 Feb 24):981-4. [Full Text/Abstract]
    Agniswamy J, Sayer JM, Weber IT, Louis JM. Terminal interface conformations modulate dimer stability prior to amino terminal autoprocessing of HIV-1 protease. Biochemistry. 2012 Feb 7; 51 (2012 Feb 7; 5; 51):1041-50. [Full Text/Abstract]
    Louis JM, Aniana A, Weber IT, Sayer JM. Inhibition of autoprocessing of natural variants and multidrug resistant mutant precursors of HIV-1 protease by clinical inhibitors. Proc Natl Acad Sci U S A. 2011 May 31; 108 (108; 22; 2011 May 31):9072-7. [Full Text/Abstract]
    Lorieau JL, Louis JM, Bax A. The complete influenza hemagglutinin fusion domain adopts a tight helical hairpin arrangement at the lipid:water interface. Proc Natl Acad Sci U S A. 2010 Jun 22; 107 (107; 25; 2010 Jun 22):11341-6. [Full Text/Abstract]
    Chung HS, Louis JM, Eaton WA. Experimental determination of upper bound for transition path times in protein folding from single-molecule photon-by-photon trajectories. Proc Natl Acad Sci U S A. 2009 Jul 21; 106 (106; 2009 Jul 21; 29):11837-44. [Full Text/Abstract]
    Tang C, Louis JM, Aniana A, Suh JY, Clore GM. Visualizing transient events in amino-terminal autoprocessing of HIV-1 protease. Nature. 2008 Oct 2; 455 (7213; 455; 2008 Oct 2):693-6. [Full Text/Abstract]
    Sayer JM, Liu F, Ishima R, Weber IT, Louis JM. Effect of the active site D25N mutation on the structure, stability, and ligand binding of the mature HIV-1 protease. J Biol Chem. 2008 May 9; 283 (2008 May 9; 19; 283):13459-70. [Full Text/Abstract]
    Louis JM, Ishima R, Torchia DA, Weber IT. HIV-1 protease: structure, dynamics, and inhibition. Adv Pharmacol. 2007; 55 (55; 2007):261-98. [Full Text/Abstract]
    Ishima R, Torchia DA, Lynch SM, Gronenborn AM, Louis JM. Solution structure of the mature HIV-1 protease monomer: insight into the tertiary fold and stability of a precursor. J Biol Chem. 2003 Oct 31; 278 (2003 Oct 31; 278; 44):43311-9. [Full Text/Abstract]
    Louis JM, Bewley CA, Clore GM. Design and properties of N(CCG)-gp41, a chimeric gp41 molecule with nanomolar HIV fusion inhibitory activity. J Biol Chem. 2001 Aug 3; 276 (2001 Aug 3; 276; 31):29485-9. [Full Text/Abstract]
    Louis JM, Clore GM, Gronenborn AM. Autoprocessing of HIV-1 protease is tightly coupled to protein folding. Nat Struct Biol. 1999 Sep; 6 (6; 1999 Sep; 9):868-75. [Full Text/Abstract]
    Louis JM, Dyda F, Nashed NT, Kimmel AR, Davies DR. Hydrophilic peptides derived from the transframe region of Gag-Pol inhibit the HIV-1 protease. Biochemistry. 1998 Feb 24; 37 (1998 Feb 24; 8; 37):2105-10. [Full Text/Abstract]
    Louis JM, Martin RG, Clore GM, Gronenborn AM. Preparation of uniformly isotope-labeled DNA oligonucleotides for NMR spectroscopy. J Biol Chem. 1998 Jan 23; 273 (1998 Jan 23; 4; 273):2374-8. [Full Text/Abstract]
    Louis JM, Nashed NT, Parris KD, Kimmel AR, Jerina DM. Kinetics and mechanism of autoprocessing of human immunodeficiency virus type 1 protease from an analog of the Gag-Pol polyprotein. Proc Natl Acad Sci U S A. 1994 Aug 16; 91 (17; 1994 Aug 16; 91):7970-4. [Full Text/Abstract]
    Louis JM, Wondrak EM, Copeland TD, Smith CA, Mora PT, Oroszlan S. Chemical synthesis and expression of the HIV-1 protease gene in E. coli. Biochem Biophys Res Commun. 1989 Feb 28; 159 (1989 Feb 28; 159; 1):87-94. [Full Text/Abstract]