U.S. Department of Health and Human Services
Joseph Shiloach

 Contact Info

Tel: 301-496-9719
Email: josephs@intra.niddk.nih.gov

 Select Experience

  • Adjunct ProfessorJohns Hopkins University, Department of Chemical and Biochemical Engineering2004
  • Research AssociateTufts University, New England Enzyme Center1975-1978
  • Ph.D.Hebrew University, Israel1975

 Related Links


Joseph Shiloach, Ph.D.

Head, Biotechnology Core
  • Biotechnology
  • Microbiology/Infectious diseases (non-viral)
  • Molecular Biology/Biochemistry


A selection of recent and significant publications can be viewed below.

Xu X, Hicks C, Li Y, Su J, Shiloach J, Kaufman JB, Fitz Y, Eichacker PQ, Cui X. Purified cell wall from the probiotic bacterium Lactobacillus gasseri activates systemic inflammation and, at higher doses, produces lethality in a rat model. Crit Care. 2014 Jul 2; 18 (2014 Jul 2; 4; 18):R140. [Full Text/Abstract]
Wang H, Li Y, Truong LN, Shi LZ, Hwang PY, He J, Do J, Cho MJ, Li H, Negrete A, Shiloach J, Berns MW, Shen B, Chen L, Wu X. CtIP maintains stability at common fragile sites and inverted repeats by end resection-independent endonuclease activity. Mol Cell. 2014 Jun 19; 54 (6; 2014 Jun 19; 54):1012-21. [Full Text/Abstract]
Xiao S, Shiloach J, Betenbaugh MJ. Engineering cells to improve protein expression. Curr Opin Struct Biol. 2014 Jun; 26 (26; 2014 Jun):32-8. [Full Text/Abstract]
Baez A, Majdalani N, Shiloach J. Production of recombinant protein by a novel oxygen-induced system in Escherichia coli. Microb Cell Fact. 2014 Apr 7; 13 (2014 Apr 7; 13; 1):50. [Full Text/Abstract]
Robbins JB, Schneerson R, Kubler-Kielb J, Keith JM, Trollfors B, Vinogradov E, Shiloach J. Toward a new vaccine for pertussis. Proc Natl Acad Sci U S A. 2014 Mar 4; 111 (2014 Mar 4; 111; 9):3213-6. [Full Text/Abstract]
Negrete A, Pai A, Shiloach J, Negrete-Virgen JA. Use of hollow fiber tangential flow filtration for the recovery and concentration of HIV virus-like particles produced in insect cells. J Virol Methods. 2014 Jan; 195 (2014 Jan; 195):240-6. [Full Text/Abstract]
Druz A, Betenbaugh M, Shiloach J. Glucose depletion activates mmu-miR-466h-5p expression through oxidative stress and inhibition of histone deacetylation. Nucleic Acids Res. 2012 Aug; 40 (2012 Aug; 40; 15):7291-302. [Full Text/Abstract]
Bellanti JA, Lin FY, Chu C, Shiloach J, Leppla SH, Benavides GA, Karpas A, Moayeri M, Guo C, Robbins JB, Schneerson R. Phase 1 study of a recombinant mutant protective antigen of Bacillus anthracis. Clin Vaccine Immunol. 2012 Feb; 19 (2012 Feb; 19; 2):140-5. [Full Text/Abstract]
Druz A, Chu C, Majors B, Santuary R, Betenbaugh M, Shiloach J. A novel microRNA mmu-miR-466h affects apoptosis regulation in mammalian cells. Biotechnol Bioeng. 2011 Jul; 108 (108; 7; 2011 Jul):1651-61. [Full Text/Abstract]
Chu C, Lugovtsev V, Lewis A, Betenbaugh M, Shiloach J. Production and antigenic properties of influenza virus from suspension MDCK-siat7e cells in a bench-scale bioreactor. Vaccine. 2010 Oct 18; 28 (28; 2010 Oct 18; 44):7193-201. [Full Text/Abstract]
Negrete A, Ng WI, Shiloach J. Glucose uptake regulation in E. coli by the small RNA SgrS: comparative analysis of E. coli K-12 (JM109 and MG1655) and E. coli B (BL21). Microb Cell Fact. 2010 Sep 28; 9 (9; 2010 Sep 28):75. [Full Text/Abstract]
Shiloach J, Reshamwala S, Noronha SB, Negrete A. Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends--example E. coli. Curr Opin Biotechnol. 2010 Feb; 21 (2010 Feb; 21; 1):21-6. [Full Text/Abstract]
Chu C, Lugovtsev V, Golding H, Betenbaugh M, Shiloach J. Conversion of MDCK cell line to suspension culture by transfecting with human siat7e gene and its application for influenza virus production. Proc Natl Acad Sci U S A. 2009 Sep 1; 106 (106; 35; 2009 Sep 1):14802-7. [Full Text/Abstract]
Chill L, Trinh L, Azadi P, Ishihara M, Sonon R, Karnaukhova E, Ophir Y, Golding B, Shiloach J. Production, purification, and characterization of human alpha1 proteinase inhibitor from Aspergillus niger. Biotechnol Bioeng. 2009 Feb 15; 102 (102; 3; 2009 Feb 15):828-44. [Full Text/Abstract]
Phue JN, Lee SJ, Trinh L, Shiloach J. Modified Escherichia coli B (BL21), a superior producer of plasmid DNA compared with Escherichia coli K (DH5alpha). Biotechnol Bioeng. 2008 Nov 1; 101 (101; 4; 2008 Nov 1):831-6. [Full Text/Abstract]
Jaluria P, Chu C, Betenbaugh M, Shiloach J. Cells by design: a mini-review of targeting cell engineering using DNA microarrays. Mol Biotechnol. 2008 Jun; 39 (2008 Jun; 2; 39):105-11. [Full Text/Abstract]
Thomas JM, Contreras JL, Wang PX, Eckhoff DE, Asiedu C, Hubbard WJ, Cartner S, Thomas FT, Robbin ML, Nadler S, Cook WJ, Sharff J, Neville DM Jr, Shiloach J. Retraction: Peritransplant tolerance induction with anti-CD3-immunotoxin: a matter of proinflammatory cytokine control. Transplantation. 2008 Mar 27; 85 (2008 Mar 27; 85; 6):920. [Full Text/Abstract]
Jaluria P, Betenbaugh M, Konstantopoulos K, Shiloach J. Enhancement of cell proliferation in various mammalian cell lines by gene insertion of a cyclin-dependent kinase homolog. BMC Biotechnol. 2007 Oct 18; 7 (7; 2007 Oct 18):71. [Full Text/Abstract]
Jaluria P, Betenbaugh M, Konstantopoulos K, Frank B, Shiloach J. Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells. Metab Eng. 2007 May; 9 (2007 May; 9; 3):241-51. [Full Text/Abstract]
Phue JN, Kedem B, Jaluria P, Shiloach J. Evaluating microarrays using a semiparametric approach: application to the central carbon metabolism of Escherichia coli BL21 and JM109. Genomics. 2007 Feb; 89 (2; 2007 Feb; 89):300-5. [Full Text/Abstract]
El-Mansi M, Cozzone AJ, Shiloach J, Eikmanns BJ. Control of carbon flux through enzymes of central and intermediary metabolism during growth of Escherichia coli on acetate. Curr Opin Microbiol. 2006 Apr; 9 (2; 9; 2006 Apr):173-9. [Full Text/Abstract]
Shiloach J, Fass R. Growing E. coli to high cell density--a historical perspective on method development. Biotechnol Adv. 2005 Jul; 23 (2005 Jul; 23; 5):345-57. [Full Text/Abstract]