U.S. Department of Health and Human Services
Alejandro Negrete
 

 Contact Info

 
Tel: 301-402-2325
Email: negretea@mail.nih.gov
 

 Select Experience

 
  • Staff ScientistNIDDK, NIH2008–Present
  • Postdoctoral ResearcherNHLBI, NIH2003–2008
  • Ph.D. in Chemical EngineeringUniversity of Birmingham, UK2003
  • M.S. in BiotechnologyMonterrey Tech (ITESM), Mexico1998
 

 Related Links

 

Juan Alejandro Negrete Virgen, Ph.D.

Staff Scientist, Biotechnology Core
Specialties
  • Biotechnology

​Publications

A selection of recent and significant publications can be viewed below.

Publications
Negrete A, Ling TC, Lyddiatt A. Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells. 2008; 2:229-234 (2:229-234; 2008):10.2174/1874070700802010229. [Full Text/Abstract]
Negrete A, Ling TC, Lyddiatt A. Production of adenoviral vectors in 293 cells: A case study of the adaptation of attached cells to grow in suspension. 2008; (2:29-35; 2008):10.2174/1874070700802010029. [Full Text/Abstract]
Negrete A, Ling TC, Lyddiatt, A. Production of adenoviral vectors and its recovery. Process Biochemistry. 2007; (42; 2007):10.1016/j.procbio.2007.05.004. [Full Text/Abstract]
Wang H, Li Y, Truong LN, Shi LZ, Hwang PY, He J, Do J, Cho MJ, Li H, Negrete A, Shiloach J, Berns MW, Shen B, Chen L, Wu X. CtIP maintains stability at common fragile sites and inverted repeats by end resection-independent endonuclease activity. Mol Cell. 2014 Jun 19; 54 (6; 2014 Jun 19; 54):1012-21. [Full Text/Abstract]
Negrete A, Pai A, Shiloach J. Use of hollow fiber tangential flow filtration for the recovery and concentration of HIV virus-like particles produced in insect cells. J Virol Methods. 2014 Jan; 195 (2014 Jan; 195):240-6. [Full Text/Abstract]
Negrete A, Majdalani N, Phue JN, Shiloach J. Reducing acetate excretion from E. coli K-12 by over-expressing the small RNA SgrS. N Biotechnol. 2013 Jan 25; 30 (30; 2; 2013 Jan 25):269-73. [Full Text/Abstract]
He J, Shi LZ, Truong LN, Lu CS, Razavian N, Li Y, Negrete A, Shiloach J, Berns MW, Wu X. Rad50 zinc hook is important for the Mre11 complex to bind chromosomal DNA double-stranded breaks and initiate various DNA damage responses. J Biol Chem. 2012 Sep 14; 287 (2012 Sep 14; 38; 287):31747-56. [Full Text/Abstract]
Phue JN, Lee SJ, Kaufman JB, Negrete A, Shiloach J. Acetate accumulation through alternative metabolic pathways in ackA (-) pta (-) poxB (-) triple mutant in E. coli B (BL21). Biotechnol Lett. 2010 Dec; 32 (32; 2010 Dec; 12):1897-903. [Full Text/Abstract]
Negrete A, Ng WI, Shiloach J. Glucose uptake regulation in E. coli by the small RNA SgrS: comparative analysis of E. coli K-12 (JM109 and MG1655) and E. coli B (BL21). Microb Cell Fact. 2010 Sep 28; 9 (9; 2010 Sep 28):75. [Full Text/Abstract]
Shiloach J, Reshamwala S, Noronha SB, Negrete A. Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends--example E. coli. Curr Opin Biotechnol. 2010 Feb; 21 (2010 Feb; 21; 1):21-6. [Full Text/Abstract]
Cecchini S, Negrete A, Virag T, Graham BS, Cohen JI, Kotin RM. Evidence of prior exposure to human bocavirus as determined by a retrospective serological study of 404 serum samples from adults in the United States. Clin Vaccine Immunol. 2009 May; 16 (16; 5; 2009 May):597-604. [Full Text/Abstract]
Negrete A, Kotin RM. Strategies for manufacturing recombinant adeno-associated virus vectors for gene therapy applications exploiting baculovirus technology. Brief Funct Genomic Proteomic. 2008 Jul; 7 (7; 4; 2008 Jul):303-11. [Full Text/Abstract]
Cecchini S, Negrete A, Kotin RM. Toward exascale production of recombinant adeno-associated virus for gene transfer applications. Gene Ther. 2008 Jun; 15 (2008 Jun; 15; 11):823-30. [Full Text/Abstract]
Negrete A, Kotin RM. Large-scale production of recombinant adeno-associated viral vectors. Methods Mol Biol. 2008; 433 (2008; 433):79-96. [Full Text/Abstract]
Negrete A, Yang LC, Mendez AF, Levy JR, Kotin RM. Economized large-scale production of high yield of rAAV for gene therapy applications exploiting baculovirus expression system. J Gene Med. 2007 Nov; 9 (2007 Nov; 9; 11):938-48. [Full Text/Abstract]
Negrete A, Kotin RM. Production of recombinant adeno-associated vectors using two bioreactor configurations at different scales. J Virol Methods. 2007 Nov; 145 (145; 2007 Nov; 2):155-61. [Full Text/Abstract]
Negrete A, Esteban G, Kotin RM. Process optimization of large-scale production of recombinant adeno-associated vectors using dielectric spectroscopy. Appl Microbiol Biotechnol. 2007 Sep; 76 (76; 4; 2007 Sep):761-72. [Full Text/Abstract]
Negrete A, Ling TC, Lyddiatt A. Aqueous two-phase recovery of bio-nanoparticles: a miniaturization study for the recovery of bacteriophage T4. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jul 1; 854 (854; 2007 Jul 1; 1-2):13-9. [Full Text/Abstract]
Gueret V, Negrete-Virgen JA, Lyddiatt A, Al-Rubeai M. Rapid titration of adenoviral infectivity by flow cytometry in batch culture of infected HEK293 cells. Cytotechnology. 2002 Jan; 38 (38; 1-3; 2002 Jan):87-97. [Full Text/Abstract]
Rito-Palomares M, Negrete A, Miranda L, Flores C, Galindo E, Serrano-Carreón L. The potential application of aqueous two-phase systems for in situ recovery of 6-pentyl-infinity-pyrone produced by Trichoderma harzianum. Enzyme Microb Technol. 2001 May 7; 28 (2001 May 7; 28; 7-8):625-631. [Full Text/Abstract]
Rito-Palomares M, Negrete A, Galindo E, Serrano-Carreon L. Aroma compounds recovery from mycelial cultures in aqueous two-phase processes. J Chromatogr B Biomed Sci Appl. 2000 Jun 23; 743 (743; 1-2; 2000 Jun 23):403-8. [Full Text/Abstract]