U.S. Department of Health and Human Services
Luis Menezes
 

 Contact Info

 
Tel: 301-451-9614
Email: menezeslf@mail.nih.gov
 

 Select Experience

 
  • Staff ScientistNIDDK, NIH2012–Present
  • Postdoctoral FellowNIDDK, NIH2009–2012
  • Postdoctoral FellowJohns Hopkins School of Medicine2005–2009
  • Human Pathology SpecialistSchool of Medicine, University of São Paulo1999–2001
  • Ph.D.School of Medicine, University of São Paulo2004
  • M.D.School of Medicine, State University of Campinas, Campinas1998
 

 Related Links

 

Luis Fernando Menezes, M.D., Ph.D.

Staff Scientist, Polycystic Kidney Disease LaboratoryKidney Diseases Branch
Specialties
  • Pathophysiology of Kidney Disease

​Research Images

Images or videos appear below. Clicking images or videos provides an expanded view.

TitleDescriptionImage
In vitro cyst formationA confocal image of mutant cells forming a cyst in vitro is depicted. Cells are stained with fluorescent markers for cell membrane (red) and nucleus (blue). The large image (center) is the surface of the cyst. The two smaller images (top and left) show vertical slices of the cyst cut through the blue and red lines, respectively. Note the empty space in the two smaller images, confirming that the cells form a cyst.In vitro cyst formationEnlarge
Hnf4α is a disease modifier.Network pathway analysis suggests that HNF4 is a node in gene networks of polycystic kidney disease (left). Kidney histology is also depicted and is confirming a modifier effect of Hnf4 in the severity of Pkd1cystogenesis (right). Note the left kidney, in which Pkd1 and Hnf4are deleted, has more and larger cyststhan the kidney in which only Pkd1 was deleted. Hnf4α is a disease modifier.Enlarge
In vitro tube formationThe lab has been generating cell lines that can be used for in vitro analysis of tube versus cyst formation. Cells isolated from Pkd1cond/cond kidney collecting ducts and subsequently immortalized form tubes when grown in collagen matrices.In vitro tube formationEnlarge
Genomics and Metabolomics of Polycystic Kidney Disease.A heatmap plot showing that a subset of differentially expressed genes separates mutant and control kidneys in both pre-cystic (P12) and cystic (P14) kidneys (left) and a Principal Component Plot showing that the pattern of metabolites expressed in the urine of control and cystic animals is different, consistent with our gene array data (right).Genomics and Metabolomics of Polycystic Kidney Disease.Enlarge