U.S. Department of Health and Human Services
Nicholas Guydosh

 Contact Info

Tel: 301-496-6261
Email: nicholas.guydosh@nih.gov

 Select Experience

  • Postdoctoral FellowJohns Hopkins University School of Medicine2009-2016
  • Ph.D.Stanford University2009
  • M.PhilUniversity of Cambridge2003
  • A.B.Harvard University2001

 Related Links


Nicholas R. Guydosh, Ph.D., Stadtman Tenure-Track Investigator

Acting Section Chief, Section on mRNA Regulation and TranslationLaboratory of Biochemistry and Genetics
  • Biomedical Engineering/Biophysics/Physics
  • Computational Biology/Bioinformatics/Biostatistics/Mathematics
  • Genetics/Genomics
  • Molecular Biology/Biochemistry
  • Virology

​Research Images

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Imaging mRNA in dividing live yeast cells. Cells are expressing a reporter mRNA that encodes MS2 stem loops in its 3’UTR and a second mRNA encoding fluorescent mKATE linked to MS2 coat protein (mKATE-MCP). Bright dots correspond to single reporter mRNA molecules in the cytoplasm that are visible because their MS2 stem loops are bound to molecules of mKATE-MCP.

Imaging mRNA in dividing live yeast cells.Enlarge

Imaging translation in a live dividing yeast cell. The cell is expressing a reporter mRNA with GCN4 repeats and a second mRNA encoding GFP linked to a Gcn4 antibody fragment (SunTag). Bright areas include sites of single polysomes where SunTags are bound to nascent Gcn4 peptides.

Imaging translation in a live dividing yeast cell.Enlarge

Splitting mammalian cell cultures.

Splitting mammalian cell cultures.Enlarge

Imaging gels and western blots.

Imaging gels and western blots.Enlarge

Running sucrose gradients.

Running sucrose gradients.Enlarge