U.S. Department of Health and Human Services
Nicholas Guydosh

 Contact Info

Tel: 301-827-2051
Email: nicholas.guydosh@nih.gov

 Select Experience

  • Postdoctoral FellowJohns Hopkins University School of Medicine2009-2016
  • Ph.D.Stanford University2009
  • M.PhilUniversity of Cambridge2003
  • A.B.Harvard University2001

 Related Links


Nicholas R. Guydosh, Ph.D., Stadtman Tenure-Track Investigator

Acting Section Chief, Section on mRNA Regulation and TranslationLaboratory of Biochemistry and Genetics
  • Biomedical Engineering/Biophysics/Physics
  • Computational Biology/Bioinformatics/Biostatistics/Mathematics
  • Genetics/Genomics
  • Molecular Biology/Biochemistry
  • Virology

​Research Images

Images or videos appear below. Clicking images or videos provides an expanded view.

Imaging mRNA in dividing live yeast cells.

Cells are expressing a reporter mRNA that encodes MS2 stem loops in its 3’UTR and a second mRNA encoding fluorescent mKATE linked to MS2 coat protein (mKATE-MCP). Bright dots correspond to single reporter mRNA molecules in the cytoplasm that are visible because their MS2 stem loops are bound to molecules of mKATE-MCP.

Imaging mRNA in dividing live yeast cellsEnlarge
Imaging translation in a live dividing yeast cell.

The cell is expressing a reporter mRNA with GCN4 repeats and a second mRNA encoding GFP linked to a Gcn4 antibody fragment (SunTag). Bright areas include sites of single polysomes where SunTags are bound to nascent Gcn4 peptides.

Imaging translation in a live dividing yeast cellEnlarge
Splitting mammalian cell cultures.

Biological safety cabinet used to develop and maintain mammalian cell lines for ribosome profiling and microscopy experiments.

Splitting mammalian cell culturesEnlarge
Imaging gels and western blots.

This apparatus is required for detecting reporter proteins and imaging gels for ribosome profiling.

Imaging gels and western blotsEnlarge
Running sucrose gradients.

Sucrose gradients are required for separating ribosomes from other heavy complexes in the cell for ribosome profiling experiments.

Running sucrose gradientsEnlarge