U.S. Department of Health and Human Services
Olga Protchenko
 

 Contact Info

 
Tel: 301-402-6024
Email: op12g@nih.gov
 

 Select Experience

 
  • Postdoctoral FellowLiver Diseases Section,  NIDDK, NIH2000–2005
  • Ph.D.A.V. Palladin Institute of Biochemistry, National Academy of Science of Ukraine1997
  • M.A.Lviv State University1988
 

 Related Links

 

Olga Protchenko, Ph.D.

Staff Scientist, Genetics and Metabolism SectionLiver Diseases Branch
Specialties
  •  Genetics/Genomics
  •  Molecular Biology/Biochemistry
  • Cell Biology/Cell Signaling
  • Chemistry/Chemical Biology
  • Microbiology/Infectious Diseases (non-viral)

​Research Images

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TitleDescriptionImage
Yeast as a tool to study heme transporters from other species.A yeast strain deficient in heme synthesis (hem1) can only grow when media are supplemented with heme precursor, 5-aminolevulinic acid (ALA), or a high concentration of heme.  Expression of the heme transporter HRG4 from the worm C.elegans allows yeast to grow at low heme concentration.Yeast as a tool to study heme transporters from other species.Enlarge
Yeast heme oxygenase Hmx1 regulates heme homeostasisThis is an indirect Immunofluorescence image of Hmx1 in a yeast cell. Hmx1 is localized to the endoplasmic reticulum, a ring-like structure around the nucleus and periphery of the cell.  Hmx1-HA was detected with (a) an anti-HA antibody, (b) a nucleus stained with DAPI, and (c) a merged image.Yeast heme oxygenase Hmx1 regulates heme homeostasisEnlarge
Heme homeostasis in the eukaryotic cell is a balance of synthesis, distribution, and degradation.This is a simplified scheme of heme homeostasis in the eukaryotic cell.  The final step of heme biosynthesis, the ferrochelatase (FECH) reaction, takes place in the mitochondria.  Newly synthesized heme is used for assembly of cytochomes (CytC) and other heme proteins located in the cytoplasm, endoplasmic reticulum (ER), lysosomes, and nucleus.  The degradation of heme to the iron, biliverdin (BV), and carbon monoxide is catalyzed by heme oxygenase associated with the ER.  Heme uptake and heme export are mediated by the function of the plasma membrane heme transporters.  Question marks signal unexplored pathways of intracellular heme transfer.Heme homeostasis in the eukaryotic cell is a balance of synthesis, distribution, and degradation.Enlarge