U.S. Department of Health and Human Services
Rafael Daniel Camerini-Otero

 Contact Info

Tel: 301-496-2710
Email: rc10d@nih.gov

 Select Experience

  • M.D., Ph.D.New York University1973
  • B.S.Massachusetts Institute of Technology1966

 Related Links


Rafael Daniel Camerini-Otero, M.D., Ph.D.

Chief, Genetics and Biochemistry Branch
Section Chief, Genetics and Biochemistry SectionGenetics and Biochemistry Branch
  • Chromosome Biology/Epigenetics
  • Computational Biology/Bioinformatics/Biostatistics/Mathematics
  • Genetics/Genomics
  • Molecular Biology/Biochemistry
  • Systems Biology


A selection of recent and significant publications can be viewed below.

Dai J, Voloshin O, Potapova S, Camerini-Otero RD. Meiotic Knockdown and Complementation Reveals Essential Role of RAD51 in Mouse Spermatogenesis. Cell Rep. 2017 Feb 7; 18 (6; 2017 Feb 7; 18):1383-1394. [Full Text/Abstract]
Davies B, Hatton E, Altemose N, Hussin JG, Pratto F, Zhang G, Hinch AG, Moralli D, Biggs D, Diaz R, Preece C, Li R, Bitoun E, Brick K, Green CM, Camerini-Otero RD, Myers SR, Donnelly P. Re-engineering the zinc fingers of PRDM9 reverses hybrid sterility in mice. Nature. 2016 Feb 3; 530 (530; 7589; 2016 Feb 3):171-6. [Full Text/Abstract]
Smagulova F, Brick K, Pu Y, Camerini-Otero RD, Petukhova GV. The evolutionary turnover of recombination hot spots contributes to speciation in mice. Genes Dev. 2016 Feb 1; 30 (2016 Feb 1; 30; 3):266-80. [Full Text/Abstract]
Pratto F, Brick K, Khil P, Smagulova F, Petukhova GV, Camerini-Otero RD. DNA recombination. Recombination initiation maps of individual human genomes. Science. 2014 Nov 14; 346 (6211; 2014 Nov 14; 346):1256442. [Full Text/Abstract]
Bugreev DV, Huang F, Mazina OM, Pezza RJ, Voloshin ON, Camerini-Otero RD, Mazin AV. HOP2-MND1 modulates RAD51 binding to nucleotides and DNA. Nat Commun. 2014 Jun 19; 5 (5; 2014 Jun 19):4198. [Full Text/Abstract]
Broering TJ, Alavattam KG, Sadreyev RI, Ichijima Y, Kato Y, Hasegawa K, Camerini-Otero RD, Lee JT, Andreassen PR, Namekawa SH. BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis. J Cell Biol. 2014 Jun 9; 205 (205; 2014 Jun 9; 5):663-75. [Full Text/Abstract]
Moktan H, Guiraldelli MF, Eyster CA, Zhao W, Lee CY, Mather T, Camerini-Otero RD, Sung P, Zhou DH, Pezza RJ. Solution structure and DNA-binding properties of the winged helix domain of the meiotic recombination HOP2 protein. J Biol Chem. 2014 May 23; 289 (2014 May 23; 289; 21):14682-91. [Full Text/Abstract]
Pezza RJ, Voloshin ON, Volodin AA, Boateng KA, Bellani MA, Mazin AV, Camerini-Otero RD. The dual role of HOP2 in mammalian meiotic homologous recombination. Nucleic Acids Res. 2014 Feb; 42 (42; 4; 2014 Feb):2346-57. [Full Text/Abstract]
Margolin G, Khil PP, Kim J, Bellani MA, Camerini-Otero RD. Integrated transcriptome analysis of mouse spermatogenesis. BMC Genomics. 2014 Jan 18; 15 (15; 2014 Jan 18):39. [Full Text/Abstract]
Zhao W, Saro D, Hammel M, Kwon Y, Xu Y, Rambo RP, Williams GJ, Chi P, Lu L, Pezza RJ, Camerini-Otero RD, Tainer JA, Wang HW, Sung P. Mechanistic insights into the role of Hop2-Mnd1 in meiotic homologous DNA pairing. Nucleic Acids Res. 2014 Jan; 42 (2; 2014 Jan; 42):906-17. [Full Text/Abstract]
Smagulova F, Brick K, Pu Y, Sengupta U, Camerini-Otero RD, Petukhova GV. Suppression of genetic recombination in the pseudoautosomal region and at subtelomeres in mice with a hypomorphic Spo11 allele. BMC Genomics. 2013 Jul 22; 14 (14; 2013 Jul 22):493. [Full Text/Abstract]
Boateng KA, Bellani MA, Gregoretti IV, Pratto F, Camerini-Otero RD. Homologous pairing preceding SPO11-mediated double-strand breaks in mice. Dev Cell. 2013 Jan 28; 24 (2; 24; 2013 Jan 28):196-205. [Full Text/Abstract]
Naumova AK, Fayer S, Leung J, Boateng KA, Camerini-Otero RD, Taketo T. Dynamics of response to asynapsis and meiotic silencing in spermatocytes from Robertsonian translocation carriers. PLoS One. 2013 Sep 16; 8 (8; 2013 Sep 16; 9):e75970. [Full Text/Abstract]
Grant J, Mahadevaiah SK, Khil P, Sangrithi MN, Royo H, Duckworth J, McCarrey JR, VandeBerg JL, Renfree MB, Taylor W, Elgar G, Camerini-Otero RD, Gilchrist MJ, Turner JM. Rsx is a metatherian RNA with Xist-like properties in X-chromosome inactivation. Nature. 2012 Jul 12; 487 (7406; 487; 2012 Jul 12):254-8. [Full Text/Abstract]
Brick K, Smagulova F, Khil P, Camerini-Otero RD, Petukhova GV. Genetic recombination is directed away from functional genomic elements in mice. Nature. 2012 May 13; 485 (2012 May 13; 485; 7400):642-5. [Full Text/Abstract]
Khil PP, Smagulova F, Brick KM, Camerini-Otero RD, Petukhova GV. Sensitive mapping of recombination hotspots using sequencing-based detection of ssDNA. Genome Res. 2012 May; 22 (2012 May; 5; 22):957-65. [Full Text/Abstract]
Fukuda T, Pratto F, Schimenti JC, Turner JM, Camerini-Otero RD, Höög C. Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis. PLoS Genet. 2012 Feb; 8 (2012 Feb; 8; 2):e1002485. [Full Text/Abstract]
Ichijima Y, Ichijima M, Lou Z, Nussenzweig A, Camerini-Otero RD, Chen J, Andreassen PR, Namekawa SH. MDC1 directs chromosome-wide silencing of the sex chromosomes in male germ cells. Genes Dev. 2011 May 1; 25 (25; 9; 2011 May 1):959-71. [Full Text/Abstract]
Smagulova F, Gregoretti IV, Brick K, Khil P, Camerini-Otero RD, Petukhova GV. Genome-wide analysis reveals novel molecular features of mouse recombination hotspots. Nature. 2011 Apr 21; 472 (2011 Apr 21; 472; 7343):375-8. [Full Text/Abstract]
Bugreev DV, Pezza RJ, Mazina OM, Voloshin ON, Camerini-Otero RD, Mazin AV. The resistance of DMC1 D-loops to dissociation may account for the DMC1 requirement in meiosis. Nat Struct Mol Biol. 2011 Jan; 18 (2011 Jan; 1; 18):56-60. [Full Text/Abstract]
Pezza RJ, Camerini-Otero RD, Bianco PR. Hop2-Mnd1 condenses DNA to stimulate the synapsis phase of DNA strand exchange. Biophys J. 2010 Dec 1; 99 (99; 2010 Dec 1; 11):3763-72. [Full Text/Abstract]
Yao H, Brick K, Evrard Y, Xiao T, Camerini-Otero RD, Felsenfeld G. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes Dev. 2010 Nov 15; 24 (2010 Nov 15; 24; 22):2543-55. [Full Text/Abstract]
Bellani MA, Boateng KA, McLeod D, Camerini-Otero RD. The expression profile of the major mouse SPO11 isoforms indicates that SPO11beta introduces double strand breaks and suggests that SPO11alpha has an additional role in prophase in both spermatocytes and oocytes. Mol Cell Biol. 2010 Sep; 30 (30; 2010 Sep; 18):4391-403. [Full Text/Abstract]
Khil PP, Camerini-Otero RD. Genetic crossovers are predicted accurately by the computed human recombination map. PLoS Genet. 2010 Jan 29; 6 (2010 Jan 29; 6; 1):e1000831. [Full Text/Abstract]
Zheng J, Khil PP, Camerini-Otero RD, Przytycka TM. Detecting sequence polymorphisms associated with meiotic recombination hotspots in the human genome. Genome Biol. 2010; 11 (2010; 10; 11):R103. [Full Text/Abstract]
Kouznetsova A, Wang H, Bellani M, Camerini-Otero RD, Jessberger R, Höög C. BRCA1-mediated chromatin silencing is limited to oocytes with a small number of asynapsed chromosomes. J Cell Sci. 2009 Jul 15; 122 (Pt 14; 2009 Jul 15; 122):2446-52. [Full Text/Abstract]
Suhasini AN, Sommers JA, Mason AC, Voloshin ON, Camerini-Otero RD, Wold MS, Brosh RM Jr. FANCJ helicase uniquely senses oxidative base damage in either strand of duplex DNA and is stimulated by replication protein A to unwind the damaged DNA substrate in a strand-specific manner. J Biol Chem. 2009 Jul 3; 284 (2009 Jul 3; 284; 27):18458-70. [Full Text/Abstract]
Volodin AA, Bocharova TN, Smirnova EA, Camerini-Otero RD. Reversibility, equilibration, and fidelity of strand exchange reaction between short oligonucleotides promoted by RecA protein from escherichia coli and human Rad51 and Dmc1 proteins. J Biol Chem. 2009 Jan 16; 284 (2009 Jan 16; 284; 3):1495-504. [Full Text/Abstract]
Khil PP, Camerini-Otero RD. Variation in patterns of human meiotic recombination. Genome Dyn. 2009; 5 (2009; 5):117-27. [Full Text/Abstract]
Usdin TB, Paciga M, Riordan T, Kuo J, Parmelee A, Petukova G, Camerini-Otero RD, Mezey E. Tuberoinfundibular Peptide of 39 residues is required for germ cell development. Endocrinology. 2008 Sep; 149 (149; 9; 2008 Sep):4292-300. [Full Text/Abstract]
Shiao MS, Khil P, Camerini-Otero RD, Shiroishi T, Moriwaki K, Yu HT, Long M. Origins of new male germ-line functions from X-derived autosomal retrogenes in the mouse. Mol Biol Evol. 2007 Oct; 24 (2007 Oct; 10; 24):2242-53. [Full Text/Abstract]
Pezza RJ, Voloshin ON, Vanevski F, Camerini-Otero RD. Hop2/Mnd1 acts on two critical steps in Dmc1-promoted homologous pairing. Genes Dev. 2007 Jul 15; 21 (2007 Jul 15; 14; 21):1758-66. [Full Text/Abstract]
Voloshin ON, Camerini-Otero RD. The DinG protein from Escherichia coli is a structure-specific helicase. J Biol Chem. 2007 Jun 22; 282 (25; 2007 Jun 22; 282):18437-47. [Full Text/Abstract]
Bannister LA, Pezza RJ, Donaldson JR, de Rooij DG, Schimenti KJ, Camerini-Otero RD, Schimenti JC. A dominant, recombination-defective allele of Dmc1 causing male-specific sterility. PLoS Biol. 2007 May; 5 (2007 May; 5):e105. [Full Text/Abstract]
Cotroneo MS, Haag JD, Zan Y, Lopez CC, Thuwajit P, Petukhova GV, Camerini-Otero RD, Gendron-Fitzpatrick A, Griep AE, Murphy CJ, Dubielzig RR, Gould MN. Characterizing a rat Brca2 knockout model. Oncogene. 2007 Mar 8; 26 (2007 Mar 8; 26; 11):1626-35. [Full Text/Abstract]
Ploquin M, Petukhova GV, Morneau D, Déry U, Bransi A, Stasiak A, Camerini-Otero RD, Masson JY. Stimulation of fission yeast and mouse Hop2-Mnd1 of the Dmc1 and Rad51 recombinases. Nucleic Acids Res. 2007; 35 (8; 35; 2007):2719-33. [Full Text/Abstract]
Liebe B, Petukhova G, Barchi M, Bellani M, Braselmann H, Nakano T, Pandita TK, Jasin M, Fornace A, Meistrich ML, Baarends WM, Schimenti J, de Lange T, Keeney S, Camerini-Otero RD, Scherthan H. Mutations that affect meiosis in male mice influence the dynamics of the mid-preleptotene and bouquet stages. Exp Cell Res. 2006 Nov 15; 312 (19; 2006 Nov 15; 312):3768-81. [Full Text/Abstract]
Sokolov MV, Smirnova NA, Camerini-Otero RD, Neumann RD, Panyutin IG. Microarray analysis of differentially expressed genes after exposure of normal human fibroblasts to ionizing radiation from an external source and from DNA-incorporated iodine-125 radionuclide. Gene. 2006 Nov 1; 382 (382; 2006 Nov 1):47-56. [Full Text/Abstract]
Pezza RJ, Petukhova GV, Ghirlando R, Camerini-Otero RD. Molecular activities of meiosis-specific proteins Hop2, Mnd1, and the Hop2-Mnd1 complex. J Biol Chem. 2006 Jul 7; 281 (2006 Jul 7; 281; 27):18426-34. [Full Text/Abstract]
Smirnova NA, Romanienko PJ, Khil PP, Camerini-Otero RD. Gene expression profiles of Spo11-/- mouse testes with spermatocytes arrested in meiotic prophase I. Reproduction. 2006 Jul; 132 (132; 1; 2006 Jul):67-77. [Full Text/Abstract]
Khil PP, Camerini-Otero RD. Molecular features and functional constraints in the evolution of the mammalian X chromosome. Crit Rev Biochem Mol Biol. 2005 Nov-Dec; 40 (40; 6; 2005 Nov-Dec):313-30. [Full Text/Abstract]
Bellani MA, Romanienko PJ, Cairatti DA, Camerini-Otero RD. SPO11 is required for sex-body formation, and Spo11 heterozygosity rescues the prophase arrest of Atm-/- spermatocytes. J Cell Sci. 2005 Aug 1; 118 (118; Pt 15; 2005 Aug 1):3233-45. [Full Text/Abstract]
Difilippantonio S, Celeste A, Fernandez-Capetillo O, Chen HT, Reina San Martin B, Van Laethem F, Yang YP, Petukhova GV, Eckhaus M, Feigenbaum L, Manova K, Kruhlak M, Camerini-Otero RD, Sharan S, Nussenzweig M, Nussenzweig A. Role of Nbs1 in the activation of the Atm kinase revealed in humanized mouse models. Nat Cell Biol. 2005 Jul; 7 (2005 Jul; 7):675-85. [Full Text/Abstract]
Petukhova GV, Pezza RJ, Vanevski F, Ploquin M, Masson JY, Camerini-Otero RD. The Hop2 and Mnd1 proteins act in concert with Rad51 and Dmc1 in meiotic recombination. Nat Struct Mol Biol. 2005 May; 12 (2005 May; 12; 5):449-53. [Full Text/Abstract]
Volodin AA, Voloshin ON, Camerini-Otero RD. Homologous recombination and RecA protein: towards a new generation of tools for genome manipulations. Trends Biotechnol. 2005 Feb; 23 (2005 Feb; 23; 2):97-102. [Full Text/Abstract]
Khil PP, Oliver B, Camerini-Otero RD. X for intersection: retrotransposition both on and off the X chromosome is more frequent. Trends Genet. 2005 Jan; 21 (2005 Jan; 21; 1):3-7. [Full Text/Abstract]
Voloshin ON, Camerini-Otero RD. Synaptic complex revisited; a homologous recombinase flips and switches bases. Mol Cell. 2004 Sep 24; 15 (6; 2004 Sep 24; 15):846-7. [Full Text/Abstract]
Lusetti SL, Voloshin ON, Inman RB, Camerini-Otero RD, Cox MM. The DinI protein stabilizes RecA protein filaments. J Biol Chem. 2004 Jul 16; 279 (2004 Jul 16; 279; 29):30037-46. [Full Text/Abstract]
Khil PP, Smirnova NA, Romanienko PJ, Camerini-Otero RD. The mouse X chromosome is enriched for sex-biased genes not subject to selection by meiotic sex chromosome inactivation. Nat Genet. 2004 Jun; 36 (2004 Jun; 6; 36):642-6. [Full Text/Abstract]
Khil PP, Obmolova G, Teplyakov A, Howard AJ, Gilliland GL, Camerini-Otero RD. Crystal structure of the Escherichia coli YjiA protein suggests a GTP-dependent regulatory function. Proteins. 2004 Feb 1; 54 (2004 Feb 1; 2; 54):371-4. [Full Text/Abstract]