U.S. Department of Health and Human Services
Rodolfo Ghirlando

 Contact Info

Tel: 301-451-8158
Email: rodolfog@intra.niddk.nih.gov

 Select Experience

  • Poste Vert FellowINSERM (National Institute of Health and Medical Research)1997
  • Visiting FellowNIDDK, NIH1995
  • Ph.D.Weizmann Institute of Science1991
  • B.Sc.University of the Witwatersrand1984
  • B.Sc. (Hons)University of the Witwatersrand1985

 Related Links


Rodolfo Ghirlando, Ph.D.

Staff Scientist, Office of the Chief, Laboratory of Molecular Biology
  • Biomedical Engineering/Biophysics/Physics
  • Molecular Biology/Biochemistry


A selection of recent and significant publications can be viewed below.

Ghirlando R, Zhao H, Balbo A, Piszczek G, Curth U, Brautigam CA, Schuck P. Measurement of the Temperature of the Resting Rotor in Analytical Ultracentrifugation. Anal Biochem. 2014 Aug 1; 458 (2014 Aug 1; 458):37-9. [Full Text/Abstract]
Zhao H, Balbo A, Metger H, Clary R, Ghirlando R, Schuck P. Improved measurement of the rotor temperature in analytical ultracentrifugation. Anal Biochem. 2014 Apr 15; 451 (2014 Apr 15; 451):69-75. [Full Text/Abstract]
Hong J, Feng H, Wang F, Ranjan A, Chen J, Jiang J, Ghirlando R, Xiao TS, Wu C, Bai Y. The catalytic subunit of the SWR1 remodeler is a histone chaperone for the H2A.Z-H2B dimer. Mol Cell. 2014 Feb 6; 53 (2014 Feb 6; 3; 53):498-505. [Full Text/Abstract]
Deshmukh L, Ghirlando R, Clore GM. Investigation of the structure and dynamics of the capsid-spacer peptide 1-nucleocapsid fragment of the HIV-1 gag polyprotein by solution NMR spectroscopy. Angew Chem Int Ed Engl. 2014 Jan 20; 53 (4; 53; 2014 Jan 20):1025-8. [Full Text/Abstract]
Zhou BR, Feng H, Ghirlando R, Kato H, Gruschus J, Bai Y. Histone H4 K16Q mutation, an acetylation mimic, causes structural disorder of its N-terminal basic patch in the nucleosome. J Mol Biol. 2012 Aug 3; 421 (2012 Aug 3; 421; 1):30-7. [Full Text/Abstract]
Inagaki S, Ghirlando R, White JF, Gvozdenovic-Jeremic J, Northup JK, Grisshammer R. Modulation of the interaction between neurotensin receptor NTS1 and Gq protein by lipid. J Mol Biol. 2012 Mar 16; 417 (417; 1-2; 2012 Mar 16):95-111. [Full Text/Abstract]
Englund EA, Wang D, Fujigaki H, Sakai H, Micklitsch CM, Ghirlando R, Martin-Manso G, Pendrak ML, Roberts DD, Durell SR, Appella DH. Programmable multivalent display of receptor ligands using peptide nucleic acid nanoscaffolds. Nat Commun. 2012 Jan 10; 3 (2012 Jan 10; 3):614. [Full Text/Abstract]
Fawzi NL, Ying J, Ghirlando R, Torchia DA, Clore GM. Atomic-resolution dynamics on the surface of amyloid-β protofibrils probed by solution NMR. Nature. 2011 Oct 30; 480 (480; 2011 Oct 30; 7376):268-72. [Full Text/Abstract]
Gloyd M, Ghirlando R, Guarné A. The role of MukE in assembling a functional MukBEF complex. J Mol Biol. 2011 Sep 30; 412 (412; 2011 Sep 30; 4):578-90. [Full Text/Abstract]
Ghirlando R. The analysis of macromolecular interactions by sedimentation equilibrium. Methods. 2011 May; 54 (54; 1; 2011 May):145-56. [Full Text/Abstract]
Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins LM, Xiao H, Tjandra N, Wu C, Bai Y. Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. Nature. 2011 Apr 14; 472 (2011 Apr 14; 7342; 472):234-7. [Full Text/Abstract]
Mouw KW, Steiner AM, Ghirlando R, Li NS, Rowland SJ, Boocock MR, Stark WM, Piccirilli JA, Rice PA. Sin resolvase catalytic activity and oligomerization state are tightly coupled. J Mol Biol. 2010 Nov 19; 404 (404; 2010 Nov 19; 1):16-33. [Full Text/Abstract]
Schwieters CD, Suh JY, Grishaev A, Ghirlando R, Takayama Y, Clore GM. Solution structure of the 128 kDa enzyme I dimer from Escherichia coli and its 146 kDa complex with HPr using residual dipolar couplings and small- and wide-angle X-ray scattering. J Am Chem Soc. 2010 Sep 22; 132 (2010 Sep 22; 37; 132):13026-45. [Full Text/Abstract]
Zhao H, Brown PH, Balbo A, Fernández-Alonso Mdel C, Polishchuck N, Chaudhry C, Mayer ML, Ghirlando R, Schuck P. Accounting for solvent signal offsets in the analysis of interferometric sedimentation velocity data. Macromol Biosci. 2010 Jul 7; 10 (2010 Jul 7; 7; 10):736-45. [Full Text/Abstract]
Giles KE, Ghirlando R, Felsenfeld G. Maintenance of a constitutive heterochromatin domain in vertebrates by a Dicer-dependent mechanism. Nat Cell Biol. 2010 Jan; 12 (2010 Jan; 12; 1):94-9; sup pp 1-6. [Full Text/Abstract]
Kato H, Gruschus J, Ghirlando R, Tjandra N, Bai Y. Characterization of the N-terminal tail domain of histone H3 in condensed nucleosome arrays by hydrogen exchange and NMR. J Am Chem Soc. 2009 Oct 28; 131 (131; 42; 2009 Oct 28):15104-5. [Full Text/Abstract]
Ghirlando R, Felsenfeld G. Hydrodynamic studies on defined heterochromatin fragments support a 30-nm fiber having six nucleosomes per turn. J Mol Biol. 2008 Mar 7; 376 (376; 2008 Mar 7; 5):1417-25. [Full Text/Abstract]
Gloyd M, Ghirlando R, Matthews LA, Guarné A. MukE and MukF form two distinct high affinity complexes. J Biol Chem. 2007 May 11; 282 (2007 May 11; 19; 282):14373-8. [Full Text/Abstract]
Ghirlando R, Litt MD, Prioleau MN, Recillas-Targa F, Felsenfeld G. Physical properties of a genomic condensed chromatin fragment. J Mol Biol. 2004 Feb 20; 336 (2004 Feb 20; 3; 336):597-605. [Full Text/Abstract]