U.S. Department of Health and Human Services
Susan Buchanan

 Contact Info

Tel: 301-594-9222
Email: skbuchan@helix.nih.gov

 Select Experience

  • Ph.D.Frankfurt University1990
  • M.S.Princeton University1986
  • B.S.Furman University1983

 Related Links

  • Structural Biology


A selection of recent and significant publications can be viewed below.

Celia H, Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Barnard TJ, Cramer WA, Lloubes R, Buchanan SK. Structural insight into the role of the Ton complex in energy transduction. Nature. 2016 Sep 21; 538 (538; 7623; 2016 Sep 21):60-65. [Full Text/Abstract]
Noinaj N, Mayclin S, Stanley AM, Jao CC, Buchanan SK. From Constructs to Crystals - Towards Structure Determination of β-barrel Outer Membrane Proteins. J Vis Exp. 2016 Jul 4; (113; 2016 Jul 4). [Full Text/Abstract]
Botos I, Majdalani N, Mayclin SJ, McCarthy JG, Lundquist K, Wojtowicz D, Barnard TJ, Gumbart JC, Buchanan SK. Structural and Functional Characterization of the LPS Transporter LptDE from Gram-Negative Pathogens. Structure. 2016 May 3; 24 (6; 2016 May 3; 24):965-976. [Full Text/Abstract]
Bakelar J, Buchanan SK, Noinaj N. The structure of the β-barrel assembly machinery complex. Science. 2016 Jan 8; 351 (2016 Jan 8; 6269; 351):180-6. [Full Text/Abstract]
Rollauer SE, Sooreshjani MA, Noinaj N, Buchanan SK. Outer membrane protein biogenesis in Gram-negative bacteria. Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5; 370 (1679; 2015 Oct 5; 370). [Full Text/Abstract]
O'Neil PK, Rollauer SE, Noinaj N, Buchanan SK. Fitting the Pieces of the β-Barrel Assembly Machinery Complex. Biochemistry. 2015 Sep 22; 54 (2015 Sep 22; 41; 54):6303-11. [Full Text/Abstract]
Cash DR, Noinaj N, Buchanan SK, Cornelissen CN. Beyond the Crystal Structure: Insight into the Function and Vaccine Potential of TbpA Expressed by Neisseria gonorrhoeae. Infect Immun. 2015 Sep 8; 83 (83; 2015 Sep 8; 11):4438-49. [Full Text/Abstract]
Kuszak AJ, Jacobs D, Gurnev PA, Shiota T, Louis JM, Lithgow T, Bezrukov SM, Rostovtseva TK, Buchanan SK. Evidence of Distinct Channel Conformations and Substrate Binding Affinities for the Mitochondrial Outer Membrane Protein Translocase Pore Tom40. J Biol Chem. 2015 Sep 2; 290 (43; 2015 Sep 2; 290):26204-26217. [Full Text/Abstract]
Noinaj N, Rollauer SE, Buchanan SK. The β-barrel membrane protein insertase machinery from Gram-negative bacteria. Curr Opin Struct Biol. 2015 Mar 18; 31 (2015 Mar 18; 31):35-42. [Full Text/Abstract]
Shen HH, Leyton DL, Shiota T, Belousoff MJ, Noinaj N, Lu J, Holt SA, Tan K, Selkrig J, Webb CT, Buchanan SK, Martin LL, Lithgow T. Reconstitution of a nanomachine driving the assembly of proteins into bacterial outer membranes. Nat Commun. 2014 Oct 24; 5 (2014 Oct 24; 5):5078. [Full Text/Abstract]
Noinaj N, Buchanan SK. Structural insights into the transport of small molecules across membranes. Curr Opin Struct Biol. 2014 Mar 26; 27 (2014 Mar 26; 27):8-15. [Full Text/Abstract]
Noinaj N, Kuszak AJ, Balusek C, Gumbart JC, Buchanan SK. Lateral opening and exit pore formation are required for BamA function. Structure. 2014 Jul 8; 22 (7; 2014 Jul 8; 22):1055-62. [Full Text/Abstract]
Noinaj N, Buchanan SK. FhaC takes a bow to FHA in the two-partner do-si-do. Mol Microbiol. 2014 Jun; 92 (6; 92; 2014 Jun):1155-8. [Full Text/Abstract]
Noinaj N, Cornelissen CN, Buchanan SK. Structural insight into the lactoferrin receptors from pathogenic Neisseria. J Struct Biol. 2013 Oct; 184 (2013 Oct; 184; 1):83-92. [Full Text/Abstract]
Noinaj N, Kuszak AJ, Gumbart JC, Lukacik P, Chang H, Easley NC, Lithgow T, Buchanan SK. Structural insight into the biogenesis of β-barrel membrane proteins. Nature. 2013 Sep 19; 501 (501; 2013 Sep 19; 7467):385-90. [Full Text/Abstract]
Lukacik P, Barnard TJ, Hinnebusch BJ, Buchanan SK. Specific targeting and killing of Gram-negative pathogens with an engineered phage lytic enzyme. Virulence. 2013 Jan 1; 4 (4; 2013 Jan 1; 1):90-1. [Full Text/Abstract]
Lukacik P, Barnard TJ, Buchanan SK. Using a bacteriocin structure to engineer a phage lysin that targets Yersinia pestis. Biochem Soc Trans. 2012 Dec 1; 40 (40; 6; 2012 Dec 1):1503-6. [Full Text/Abstract]
Noinaj N, Buchanan SK, Cornelissen CN. The transferrin-iron import system from pathogenic Neisseria species. Mol Microbiol. 2012 Oct; 86 (86; 2012 Oct; 2):246-57. [Full Text/Abstract]
Webb CT, Selkrig J, Perry AJ, Noinaj N, Buchanan SK, Lithgow T. Dynamic association of BAM complex modules includes surface exposure of the lipoprotein BamC. J Mol Biol. 2012 Sep 28; 422 (422; 2012 Sep 28; 4):545-55. [Full Text/Abstract]
Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK. Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis. Structure. 2012 Jul 3; 20 (20; 2012 Jul 3; 7):1233-43. [Full Text/Abstract]
Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK. Structural engineering of a phage lysin that targets gram-negative pathogens. Proc Natl Acad Sci U S A. 2012 Jun 19; 109 (25; 109; 2012 Jun 19):9857-62. [Full Text/Abstract]
Noinaj N, Easley NC, Oke M, Mizuno N, Gumbart J, Boura E, Steere AN, Zak O, Aisen P, Tajkhorshid E, Evans RW, Gorringe AR, Mason AB, Steven AC, Buchanan SK. Structural basis for iron piracy by pathogenic Neisseria. Nature. 2012 Feb 12; 483 (2012 Feb 12; 483; 7387):53-8. [Full Text/Abstract]
Barnard TJ, Gumbart J, Peterson JH, Noinaj N, Easley NC, Dautin N, Kuszak AJ, Tajkhorshid E, Bernstein HD, Buchanan SK. Molecular basis for the activation of a catalytic asparagine residue in a self-cleaving bacterial autotransporter. J Mol Biol. 2012 Jan 6; 415 (2012 Jan 6; 415; 1):128-42. [Full Text/Abstract]
Yamashita S, Lukacik P, Barnard TJ, Noinaj N, Felek S, Tsang TM, Krukonis ES, Hinnebusch BJ, Buchanan SK. Structural insights into Ail-mediated adhesion in Yersinia pestis. Structure. 2011 Nov 9; 19 (19; 2011 Nov 9; 11):1672-82. [Full Text/Abstract]
Fairman JW, Noinaj N, Buchanan SK. The structural biology of β-barrel membrane proteins: a summary of recent reports. Curr Opin Struct Biol. 2011 Aug; 21 (4; 21; 2011 Aug):523-31. [Full Text/Abstract]
Tong J, Dolezal P, Selkrig J, Crawford S, Simpson AG, Noinaj N, Buchanan SK, Gabriel K, Lithgow T. Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana. Mol Biol Evol. 2011 May; 28 (28; 5; 2011 May):1581-91. [Full Text/Abstract]
Noinaj N, Fairman JW, Buchanan SK. The crystal structure of BamB suggests interactions with BamA and its role within the BAM complex. J Mol Biol. 2011 Mar 25; 407 (2; 407; 2011 Mar 25):248-60. [Full Text/Abstract]
Noinaj N, Guillier M, Barnard TJ, Buchanan SK. TonB-dependent transporters: regulation, structure, and function. Annu Rev Microbiol. 2010; 64 (2010; 64):43-60. [Full Text/Abstract]
Udho E, Jakes KS, Buchanan SK, James KJ, Jiang X, Klebba PE, Finkelstein A. Reconstitution of bacterial outer membrane TonB-dependent transporters in planar lipid bilayer membranes. Proc Natl Acad Sci U S A. 2009 Dec 22; 106 (106; 51; 2009 Dec 22):21990-5. [Full Text/Abstract]
Dagley MJ, Dolezal P, Likic VA, Smid O, Purcell AW, Buchanan SK, Tachezy J, Lithgow T. The protein import channel in the outer mitosomal membrane of Giardia intestinalis. Mol Biol Evol. 2009 Sep; 26 (9; 26; 2009 Sep):1941-7. [Full Text/Abstract]
Gatsos X, Perry AJ, Anwari K, Dolezal P, Wolynec PP, Likić VA, Purcell AW, Buchanan SK, Lithgow T. Protein secretion and outer membrane assembly in Alphaproteobacteria. FEMS Microbiol Rev. 2008 Nov; 32 (32; 6; 2008 Nov):995-1009. [Full Text/Abstract]
Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK. Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Nat Struct Mol Biol. 2007 Dec; 14 (14; 2007 Dec; 12):1214-20. [Full Text/Abstract]
Buchanan SK, Lukacik P, Grizot S, Ghirlando R, Ali MM, Barnard TJ, Jakes KS, Kienker PK, Esser L. Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import. EMBO J. 2007 May 16; 26 (10; 26; 2007 May 16):2594-604. [Full Text/Abstract]