U.S. Department of Health and Human Services

7th Annual MMPC Course: Isotope Tracers in Metabolic Research

11/10/2014 12:00 AM
11/14/2014 12:00 AM
Laila Boesinger
Department of Nutrition - School of Medicine
10900 Euclid Avenue - WG48
Cleveland, OH 44106-4954
Wyndham Cleveland at PlayhouseSquare

Event Details

Course Directors

Robert Wolfe, PhD and Henri Brunengraber, MD, PhD

Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)

This is the seventh annual offering of a course that provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in man and in animals. The course is designed for beginners as well as those with experience who wish to expand their capabilities to more sophisticated problems. The faculty is well-versed in a variety of applications and methodologies.

Techniques will be presented for investigating whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation. The basic aspects of modeling will be considered, as well as specific applications in the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Applications in humans and animal models (particularly mouse) will be considered.

Course material will be available for download from http://www.mmpc.org/shared/tracers.aspx

Problems and discussion questions will highlight key concepts. In addition to organized sessions, individual attendees will have ample opportunities for personal interaction with faculty members in the form of one-on-one mentoring sessions to discuss their research projects in more depth.

Director & Faculty Information


Douglas E. BEFROY, DPhil. New Haven, CT
Henri BRUNENGRABER, MD PhD Cleveland, OH -- Course Co-Director
Gary CLINE, PhD New Haven, CT
Joanne KELLEHER, PhD Boston, MA
Maren LAUGHLIN, PhD Bethesda, MD
Owen McGUINNESS, PhD Nashville, TN
Matthew MERRITT, PhD Dallas, TX
Elizabeth PARKS, PhD Columbia, MO
Stephen PREVIS, PhD Rahway, NJ
Michelle PUCHOWICZ, PhD Cleveland, OH
Robert R. WOLFE, PhD Little Rock, AR -- Course Director

Sponsored by

This course is supported by the Mouse Metabolic Phenotyping Centers (by grant 5U24DK076169-09).

Registration & Accommodations

Registration and Payment must be received prior to October 9, 2014

Registration Form

Download the Registration Form (PDF, 718 KB).
Registration Fee
Registration fee includes: ~ breakfast, lunch and snacks (Nov 10 - 14). Limited to 100 participants.
Students / Post docs                    $400
Academic / Government scientists $750
Industry scientists                        $1250

Please Make Check Payable To

Case Western Reserve University
Ref.: 7th Annual MMPC Course

Mail To

Case Western Reserve University
Attn: Laila Boesinger
Department of Nutrition - School of Medicine
10900 Euclid Avenue - WG48
Cleveland, OH 44106-4954 (USA)
Payable by personal, company or institutional check ONLY by Monday October 13, 2014


Cleveland, Ohio
1 (877) 999-3223
Reservations Identifier: 7th Annual isotope Tracers Course


​Monday, November 10, 2014

  • Basic characteristics of radioactive, stable isotope tracers.
  • General principles of mass spectrometry.
    • Isotopic enrichment using GC-MS.
  • Methods of mass spectrometry analysis.
    • Measurement of specific activity.

Tuesday, November 11, 2014

  • Tracer kinetics (single pool models).
    • Oxidation and synthesis rates.
  • Glucose metabolism (clamp studies).
    • Lipid metabolism (basic kinetics).

Wednesday, November 12, 2014

  • Pathway fluxes using NMR isotopomer analysis.
    • Methods in protein metabolism.

Thursday, November 13, 2014

  • Energy expenditure with doubly labeled water.
    • Synthesis rates with deuterated water:
      • proteins
      • fatty acids sterols
      • glucose
      • nucleic acids.
  • Mass isotopomer distribution analysis:
    • polymer synthesis
    • multiple flux pathways
    • TCA cycle, anaplerosis.

Friday, November 14, 2014

  • Pathway discovery via association of isotopomer analysis and metabolomics.
    • Inherently difficult problems.



Classes will be held at Wyndham Cleveland at Playhouse Square. Please plan to arrive Sunday night, November 9th, and to leave Friday afternoon on Friday, November 14th.
The Cleveland Hopkins Int’l Airport is only within 20 minutes from the Wyndham Hotel by public transit.






  • 1260 Euclid Avenue
  • OH 44106-4954


Laila Boesinger
Department of Nutrition - School of Medicine
10900 Euclid Avenue - WG48
Cleveland, OH 44106-4954