U.S. Department of Health and Human Services
8th Annual Course on Isotope Tracers in Metabolic Research

8th Annual Course on Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis

11/16/2015 10:00 AM
11/20/2015 10:00 AM
Yes
No
Maren R. Laughlin, PhD
NIDDK, NIH
301-594-8802
Cleveland
Wyndham Cleveland at PlayhouseSquare

Event Details

Background

This is the eighth annual offering of a course that provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in man and in animals. The course is designed for beginners as well as those with experience who wish to expand their capabilities to more sophisticated problems. The faculty is well-versed in a variety of applications and methodologies.
 
Techniques will be presented for investigating whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation. The basic aspects of modeling will be considered, as well as specific applications in the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Applications in humans and animal models (particularly mouse) will be considered.
 
Problems and discussion questions will highlight key concepts. In addition to organized sessions, individual attendees will have ample opportunities for personal interaction with faculty members in the form of one-on-one mentoring sessions to discuss their research projects in more depth.  
 

Course Directors

Robert Wolfe, Ph.D., Owen McGuinness, Ph.D., and Henri Brunengraber, M.D., Ph.D.

 

Course material

Materials will be available for download from http://www.mmpc.org/shared/courses.aspx.
   

Sponsors

Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)

Registration Deadline

Not Available

Agenda

Agenda is currently unavailable.

Directions/Travel

Directions/Travel information is currently unavailable.

Minutes

Minutes are currently unavailable.

Attendees

Attendees are currently unavailable.

Abstracts

November 16-20, 2014
Wyndham Cleveland at PlayhouseSquare
1260 Euclid Avenue
Cleveland, Ohio
Course Directors: Robert Wolfe, PhD and Henri Brunengraber, MD, PhD
 
Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)
 
This is the seventh annual offering of a course that provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in man and in animals. The course is designed for beginners as well as those with experience who wish to expand their capabilities to more sophisticated problems. The faculty is well-versed in a variety of applications and methodologies.
 
Techniques will be presented for investigating whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation. The basic aspects of modeling will be considered, as well as specific applications in the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Applications in humans and animal models (particularly mouse) will be considered.
 
Course material will be available for download from http://www.mmpc.org/shared/tracers.aspx
 
Problems and discussion questions will highlight key concepts. In addition to organized sessions, individual attendees will have ample opportunities for personal interaction with faculty members in the form of one-on-one mentoring sessions to discuss their research projects in more depth.

Location

Line
  • 1260 Euclid Avenue
  • OH 44115
Webinar

Contacts

Line Maren R. Laughlin, PhD
NIDDK, NIH
301-594-8802