U.S. Department of Health and Human Services

Laboratory of Molecular Biology

Susan K. Buchanan, Ph.D., Chief


A selection of recent and significant publications can be viewed below.

Celia H, Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Barnard TJ, Cramer WA, Lloubes R, Buchanan SK. Structural insight into the role of the Ton complex in energy transduction. Nature. 2016 Sep 21; 538 (538; 7623; 2016 Sep 21):60-65. [Full Text/Abstract]
Noinaj N, Mayclin S, Stanley AM, Jao CC, Buchanan SK. From Constructs to Crystals - Towards Structure Determination of β-barrel Outer Membrane Proteins. J Vis Exp. 2016 Jul 4; (113; 2016 Jul 4). [Full Text/Abstract]
Kersey RK, Brodigan TM, Fukushige T, Krause MW. Regulation of UNC-130/FOXD-mediated mesodermal patterning in C. elegans. Dev Biol. 2016 Jun 21; 416 (2; 2016 Jun 21; 416):300-11. [Full Text/Abstract]
Atwood BL, Woolnough JL, Lefevre GM, Saint Just Ribeiro M, Felsenfeld G, Giles KE. Human Argonaute 2 Is Tethered to Ribosomal RNA through MicroRNA Interactions. J Biol Chem. 2016 Jun 10; 291 (291; 2016 Jun 10; 34):17919-28. [Full Text/Abstract]
Botos I, Majdalani N, Mayclin SJ, McCarthy JG, Lundquist K, Wojtowicz D, Barnard TJ, Gumbart JC, Buchanan SK. Structural and Functional Characterization of the LPS Transporter LptDE from Gram-Negative Pathogens. Structure. 2016 May 3; 24 (6; 2016 May 3; 24):965-976. [Full Text/Abstract]
Ghirlando R, Felsenfeld G. CTCF: making the right connections. Genes Dev. 2016 Apr 15; 30 (2016 Apr 15; 30; 8):881-91. [Full Text/Abstract]
Thompson KW, Joshi P, Dymond JS, Gorrepati L, Smith HE, Krause MW, Eisenmann DM. The Paired-box protein PAX-3 regulates the choice between lateral and ventral epidermal cell fates in C. elegans. Dev Biol. 2016 Mar 4; 412 (2016 Mar 4; 412; 2):191-207. [Full Text/Abstract]
Bakelar J, Buchanan SK, Noinaj N. The structure of the β-barrel assembly machinery complex. Science. 2016 Jan 8; 351 (2016 Jan 8; 6269; 351):180-6. [Full Text/Abstract]
Rollauer SE, Sooreshjani MA, Noinaj N, Buchanan SK. Outer membrane protein biogenesis in Gram-negative bacteria. Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5; 370 (1679; 2015 Oct 5; 370). [Full Text/Abstract]
Wongtrakoongate P, Riddick G, Fucharoen S, Felsenfeld G. Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes. PLoS Genet. 2015 Oct; 11 (2015 Oct; 10; 11):e1005615. [Full Text/Abstract]
O'Neil PK, Rollauer SE, Noinaj N, Buchanan SK. Fitting the Pieces of the β-Barrel Assembly Machinery Complex. Biochemistry. 2015 Sep 22; 54 (2015 Sep 22; 41; 54):6303-11. [Full Text/Abstract]
Cash DR, Noinaj N, Buchanan SK, Cornelissen CN. Beyond the Crystal Structure: Insight into the Function and Vaccine Potential of TbpA Expressed by Neisseria gonorrhoeae. Infect Immun. 2015 Sep 8; 83 (83; 2015 Sep 8; 11):4438-49. [Full Text/Abstract]
Xiao T, Wongtrakoongate P, Trainor C, Felsenfeld G. CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites. Cell Rep. 2015 Sep 8; 12 (2015 Sep 8; 12; 10):1704-14. [Full Text/Abstract]
Kuszak AJ, Jacobs D, Gurnev PA, Shiota T, Louis JM, Lithgow T, Bezrukov SM, Rostovtseva TK, Buchanan SK. Evidence of Distinct Channel Conformations and Substrate Binding Affinities for the Mitochondrial Outer Membrane Protein Translocase Pore Tom40. J Biol Chem. 2015 Sep 2; 290 (43; 2015 Sep 2; 290):26204-26217. [Full Text/Abstract]
Singh SK, Gellert M. Role of RAG1 autoubiquitination in V(D)J recombination. Proc Natl Acad Sci U S A. 2015 Jun 29; 112 (2015 Jun 29; 28; 112):8579-8583. [Full Text/Abstract]
Gorrepati L, Krause MW, Chen W, Brodigan TM, Correa-Mendez M, Eisenmann DM. Identification of Wnt Pathway Target Genes Regulating the Division and Differentiation of Larval Seam Cells and Vulval Precursor Cells in Caenorhabditis elegans. G3 (Bethesda). 2015 Jun 5; 5 (2015 Jun 5; 8; 5):1551-66. [Full Text/Abstract]
Liu Z, Shi H, Szymczak LC, Aydin T, Yun S, Constas K, Schaeffer A, Ranjan S, Kubba S, Alam E, McMahon DE, He J, Shwartz N, Tian C, Plavskin Y, Lindy A, Dad NA, Sheth S, Amin NM, Zimmerman S, Liu D, Schwarz EM, Smith H, Krause MW, Liu J. Promotion of bone morphogenetic protein signaling by tetraspanins and glycosphingolipids. PLoS Genet. 2015 May; 11 (2015 May; 5; 11):e1005221. [Full Text/Abstract]
Lapkouski M, Chuenchor W, Kim MS, Gellert M, Yang W. Assembly Pathway and Characterization of the RAG1/2-DNA Paired and Signal-end Complexes. J Biol Chem. 2015 Apr 22; 290 (23; 2015 Apr 22; 290):14618-25. [Full Text/Abstract]
Noinaj N, Rollauer SE, Buchanan SK. The β-barrel membrane protein insertase machinery from Gram-negative bacteria. Curr Opin Struct Biol. 2015 Mar 18; 31 (2015 Mar 18; 31):35-42. [Full Text/Abstract]
Kim MS, Lapkouski M, Yang W, Gellert M. Crystal structure of the V(D)J recombinase RAG1-RAG2. Nature. 2015 Feb 18; 518 (7540; 518; 2015 Feb 18):507-511. [Full Text/Abstract]
Xu Z, Lefevre GM, Gavrilova O, Foster St Claire MB, Riddick G, Felsenfeld G. Mapping of long-range INS promoter interactions reveals a role for calcium-activated chloride channel ANO1 in insulin secretion. Proc Natl Acad Sci U S A. 2014 Nov 10; 111 (2014 Nov 10; 111; 47):16760-5. [Full Text/Abstract]
Shen HH, Leyton DL, Shiota T, Belousoff MJ, Noinaj N, Lu J, Holt SA, Tan K, Selkrig J, Webb CT, Buchanan SK, Martin LL, Lithgow T. Reconstitution of a nanomachine driving the assembly of proteins into bacterial outer membranes. Nat Commun. 2014 Oct 24; 5 (2014 Oct 24; 5):5078. [Full Text/Abstract]
Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux G. Scalable and Versatile Genome Editing Using Linear DNAs with Micro-Homology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Sep 23; 198 (2014 Sep 23; 4; 198):1347-56. [Full Text/Abstract]
Zhang J, Ferré-D'Amaré AR. Dramatic improvement of crystals of large RNAs by cation replacement and dehydration. Structure. 2014 Sep 2; 22 (2014 Sep 2; 9; 22):1363-1371. [Full Text/Abstract]
Vecchiarelli AG, Li M, Mizuuchi M, Mizuuchi K. Differential affinities of MinD and MinE to anionic phospholipid influence Min Patterning dynamics in vitro. Mol Microbiol. 2014 Aug; 93 (93; 2014 Aug; 3):453-63. [Full Text/Abstract]
Noinaj N, Buchanan SK. Structural insights into the transport of small molecules across membranes. Curr Opin Struct Biol. 2014 Mar 26; 27 (2014 Mar 26; 27):8-15. [Full Text/Abstract]
Zhang T, Ye Y. The final moments of misfolded proteins en route to the proteasome. DNA Cell Biol. 2014 Aug; 33 (8; 33; 2014 Aug):477-83. [Full Text/Abstract]
Hickman AB, Ewis HE, Li X, Knapp JA, Laver T, Doss AL, Tolun G, Steven AC, Grishaev A, Bax A, Atkinson PW, Craig NL, Dyda F. Structural basis of hAT transposon end recognition by Hermes, an octameric DNA transposase from Musca domestica. Cell. 2014 Jul 17; 158 (2014 Jul 17; 2; 158):353-367. [Full Text/Abstract]
Noinaj N, Kuszak AJ, Balusek C, Gumbart JC, Buchanan SK. Lateral opening and exit pore formation are required for BamA function. Structure. 2014 Jul 8; 22 (7; 2014 Jul 8; 22):1055-62. [Full Text/Abstract]
Zhang J, Ferré-D'Amaré AR. Direct evaluation of tRNA aminoacylation status by the T-box riboswitch using tRNA-mRNA stacking and steric readout. Mol Cell. 2014 Jul 3; 55 (55; 1; 2014 Jul 3):148-55. [Full Text/Abstract]
Noinaj N, Buchanan SK. FhaC takes a bow to FHA in the two-partner do-si-do. Mol Microbiol. 2014 Jun; 92 (6; 92; 2014 Jun):1155-8. [Full Text/Abstract]
Jackson BM, Abete-Luzi P, Krause MW, Eisenmann DM. Use of an Activated Beta-Catenin to Identify Wnt Pathway Target Genes in Caenorhabditis elegans, Including a Subset of Collagen Genes Expressed in Late Larval Development. G3 (Bethesda). 2014 Apr 16; 4 (2014 Apr 16; 4):733-47. [Full Text/Abstract]
Vecchiarelli AG, Neuman KC, Mizuuchi K. A propagating ATPase gradient drives transport of surface-confined cellular cargo. Proc Natl Acad Sci U S A. 2014 Apr 1; 111 (2014 Apr 1; 111; 13):4880-5. [Full Text/Abstract]
Christianson JC, Ye Y. Cleaning up in the endoplasmic reticulum: ubiquitin in charge. Nat Struct Mol Biol. 2014 Apr; 21 (4; 21; 2014 Apr):325-35. [Full Text/Abstract]
Yan L, Liu W, Zhang H, Liu C, Shang Y, Ye Y, Zhang X, Li W. Ube2g2-gp78-mediated HERP polyubiquitylation is involved in ER stress recovery. J Cell Sci. 2014 Apr 1; 127 (127; Pt 7; 2014 Apr 1):1417-27. [Full Text/Abstract]
Huang CH, Chu YR, Ye Y, Chen X. Role of HERP and a HERP-related protein in HRD1-dependent protein degradation at the endoplasmic reticulum. J Biol Chem. 2014 Feb 14; 289 (2014 Feb 14; 7; 289):4444-54. [Full Text/Abstract]
Lee JG, Kim W, Gygi S, Ye Y. Characterization of the deubiquitinating activity of USP19 and its role in endoplasmic reticulum-associated degradation. J Biol Chem. 2014 Feb 7; 289 (6; 289; 2014 Feb 7):3510-7. [Full Text/Abstract]
Zhuang X, Semenova E, Maric D, Craigie R. Dephosphorylation of barrier-to-autointegration factor by protein phosphatase 4 and its role in cell mitosis. J Biol Chem. 2014 Jan 10; 289 (2014 Jan 10; 2; 289):1119-27. [Full Text/Abstract]
Liu W, Shang Y, Zeng Y, Liu C, Li Y, Zhai L, Wang P, Lou J, Xu P, Ye Y, Li W. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains. EMBO J. 2014 Jan 7; 33 (2014 Jan 7; 33; 1):46-61. [Full Text/Abstract]
Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y. USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. Elife. 2014; 3 (2014; 3):e01369. [Full Text/Abstract]
Felsenfeld G. The evolution of epigenetics. Perspect Biol Med. 2014 Winter; 57 (1; 2014 Winter; 57):132-48. [Full Text/Abstract]
Ghosh SK, Bond MR, Love DC, Ashwell GG, Krause MW, Hanover JA. Disruption of O-GlcNAc Cycling in C. elegans Perturbs Nucleotide Sugar Pools and Complex Glycans. Front Endocrinol (Lausanne). 2014 Nov 24; 5 (2014 Nov 24; 5):197. [Full Text/Abstract]
Felsenfeld G. A brief history of epigenetics. Cold Spring Harb Perspect Biol. 2014 Jan 1; 6 (6; 2014 Jan 1; 1). [Full Text/Abstract]
Kallampallil J, Wood SJ, O'Dempsey T, Craigie RJ. Nematode infection mimicking paratesticular malignancy. BMJ Case Rep. 2013 (2013). [Full Text/Abstract]
Ghirlando R, Felsenfeld G. Physical chemistry of nucleic acids and their complexes. Biopolymers. 2013 Dec; 99 (2013 Dec; 12; 99):910-5. [Full Text/Abstract]
Noinaj N, Cornelissen CN, Buchanan SK. Structural insight into the lactoferrin receptors from pathogenic Neisseria. J Struct Biol. 2013 Oct; 184 (2013 Oct; 184; 1):83-92. [Full Text/Abstract]
Noinaj N, Kuszak AJ, Gumbart JC, Lukacik P, Chang H, Easley NC, Lithgow T, Buchanan SK. Structural insight into the biogenesis of β-barrel membrane proteins. Nature. 2013 Sep 19; 501 (501; 2013 Sep 19; 7467):385-90. [Full Text/Abstract]
Huang CH, Hsiao HT, Chu YR, Ye Y, Chen X. Derlin2 facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum. J Biol Chem. 2013 Aug 30; 288 (35; 288; 2013 Aug 30):25330-9. [Full Text/Abstract]
Zhang J, Ferré-D'Amaré AR. Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA. Nature. 2013 Aug 15; 500 (500; 2013 Aug 15; 7462):363-6. [Full Text/Abstract]
Chandler M, de la Cruz F, Dyda F, Hickman AB, Moncalian G, Ton-Hoang B. Breaking and joining single-stranded DNA: the HUH endonuclease superfamily. Nat Rev Microbiol. 2013 Aug; 11 (8; 2013 Aug; 11):525-38. [Full Text/Abstract]