Publications

A selection of recent and significant publications can be viewed below.

OLFM4-RET fusion is an oncogenic driver in small intestine adenocarcinoma.: Liu W, Li H, Aerbajinai W, Botos I, Rodgers GP.; Oncogene (2022 Jan) 41:72-82. Abstract/Full Text
Building Better Barrels - β-barrel Biogenesis and Insertion in Bacteria and Mitochondria.: Diederichs KA, Buchanan SK, Botos I.; J Mol Biol (2021 Aug 6) 433:166894. Abstract/Full Text
Conformational constraints of cyclopentane peptide nucleic acids facilitate tunable binding to DNA.: Zheng H, Botos I, Clausse V, Nikolayevskiy H, Rastede EE, Fouz MF, Mazur SJ, Appella DH.; Nucleic Acids Res (2021 Jan 25) 49:713-725. Abstract/Full Text
Structural insight into toxin secretion by contact-dependent growth inhibition transporters.: Guerin J, Botos I, Zhang Z, Lundquist K, Gumbart JC, Buchanan SK.; Elife (2020 Oct 22) 9. Abstract/Full Text
Structural insight into mitochondrial β-barrel outer membrane protein biogenesis.: Diederichs KA, Ni X, Rollauer SE, Botos I, Tan X, King MS, Kunji ERS, Jiang J, Buchanan SK.; Nat Commun (2020 Jul 3) 11:3290. Abstract/Full Text
Cryo-EM structure of substrate-free E. coli Lon protease provides insights into the dynamics of Lon machinery.: Botos I, Lountos GT, Wu W, Cherry S, Ghirlando R, Kudzhaev AM, Rotanova TV, de Val N, Tropea JE, Gustchina A, Wlodawer A.; Curr Res Struct Biol (2019 Nov) 1:13-20. Abstract/Full Text
Cryo-EM structure of the bacterial Ton motor subcomplex ExbB-ExbD provides information on structure and stoichiometry.: Celia H, Botos I, Ni X, Fox T, De Val N, Lloubes R, Jiang J, Buchanan SK.; Commun Biol (2019) 2:358. Abstract/Full Text
New insights into structural and functional relationships between LonA proteases and ClpB chaperones.: Rotanova TV, Andrianova AG, Kudzhaev AM, Li M, Botos I, Wlodawer A, Gustchina A.; FEBS Open Bio (2019 Sep) 9:1536-1551. Abstract/Full Text
Insertion of proteins and lipopolysaccharide into the bacterial outer membrane.: Botos I, Noinaj N, Buchanan SK.; Philos Trans R Soc Lond B Biol Sci (2017 Aug 5) 372. Abstract/Full Text
Structure and dynamics of a constitutively active neurotensin receptor.: Krumm BE, Lee S, Bhattacharya S, Botos I, White CF, Du H, Vaidehi N, Grisshammer R.; Sci Rep (2016 Dec 7) 6:38564. Abstract/Full Text
Structure of the NPr:EIN(Ntr) Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems.: Strickland M, Stanley AM, Wang G, Botos I, Schwieters CD, Buchanan SK, Peterkofsky A, Tjandra N.; Structure (2016 Dec 6) 24:2127-2137. Abstract/Full Text
Structural insight into the role of the Ton complex in energy transduction.: Celia H, Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Barnard TJ, Cramer WA, Lloubes R, Buchanan SK.; Nature (2016 Oct 6) 538:60-65. Abstract/Full Text
Structural and Functional Characterization of the LPS Transporter LptDE from Gram-Negative Pathogens.: Botos I, Majdalani N, Mayclin SJ, McCarthy JG, Lundquist K, Wojtowicz D, Barnard TJ, Gumbart JC, Buchanan SK.; Structure (2016 Jun 7) 24:965-976. Abstract/Full Text
The structural biology of Toll-like receptors.: Botos I, Segal DM, Davies DR.; Structure (2011 Apr 13) 19:447-59. Abstract/Full Text
The toll-like receptor 3:dsRNA signaling complex.: Botos I, Liu L, Wang Y, Segal DM, Davies DR.; Biochim Biophys Acta (2009 Sep-Oct) 1789:667-74. Abstract/Full Text
Structural basis of toll-like receptor 3 signaling with double-stranded RNA.: Liu L, Botos I, Wang Y, Leonard JN, Shiloach J, Segal DM, Davies DR.; Science (2008 Apr 18) 320:379-81. Abstract/Full Text
Limited proteolysis of E. coli ATP-dependent protease Lon - a unified view of the subunit architecture and characterization of isolated enzyme fragments.: Melnikov EE, Andrianova AG, Morozkin AD, Stepnov AA, Makhovskaya OV, Botos I, Gustchina A, Wlodawer A, Rotanova TV.; Acta Biochim Pol (2008) 55:281-96. Abstract/Full Text
The expanding diversity of serine hydrolases.: Botos I, Wlodawer A.; Curr Opin Struct Biol (2007 Dec) 17:683-90. Abstract/Full Text
Atomic-resolution crystal structure of the antiviral lectin scytovirin.: Moulaei T, Botos I, Ziółkowska NE, Bokesch HR, Krumpe LR, McKee TC, O'Keefe BR, Dauter Z, Wlodawer A.; Protein Sci (2007 Dec) 16:2756-60. Abstract/Full Text
[Forms of LonB protease from Archaeoglobus fulgidus devoid of the transmembrane domain: the contribution of the quaternary structure to the regulation of enzyme proteolytic activity].: Makhovskaia OV, Kozlov S, Botos I, Stepnov AA, Andrianova AG, Gushchina AE, Vlodaver A, Mel'nikov EE, Rotanova TV.; Bioorg Khim (2007 Nov-Dec) 33:657-60. Abstract/Full Text
Slicing a protease: structural features of the ATP-dependent Lon proteases gleaned from investigations of isolated domains.: Rotanova TV, Botos I, Melnikov EE, Rasulova F, Gustchina A, Maurizi MR, Wlodawer A.; Protein Sci (2006 Aug) 15:1815-28. Abstract/Full Text
Overexpression and purification of scytovirin, a potent, novel anti-HIV protein from the cultured cyanobacterium Scytonema varium.: Xiong C, O'Keefe BR, Botos I, Wlodawer A, McMahon JB.; Protein Expr Purif (2006 Apr) 46:233-9. Abstract/Full Text
The molecular structure of the TLR3 extracellular domain.: Bell JK, Botos I, Hall PR, Askins J, Shiloach J, Davies DR, Segal DM.; J Endotoxin Res (2006) 12:375-8. Abstract/Full Text
Atomic-resolution crystal structure of the proteolytic domain of Archaeoglobus fulgidus lon reveals the conformational variability in the active sites of lon proteases.: Botos I, Melnikov EE, Cherry S, Kozlov S, Makhovskaya OV, Tropea JE, Gustchina A, Rotanova TV, Wlodawer A.; J Mol Biol (2005 Aug 5) 351:144-57. Abstract/Full Text
The molecular structure of the Toll-like receptor 3 ligand-binding domain.: Bell JK, Botos I, Hall PR, Askins J, Shiloach J, Segal DM, Davies DR.; Proc Natl Acad Sci U S A (2005 Aug 2) 102:10976-80. Abstract/Full Text
Pathological crystallography: case studies of several unusual macromolecular crystals.: Dauter Z, Botos I, LaRonde-LeBlanc N, Wlodawer A.; Acta Crystallogr D Biol Crystallogr (2005 Jul) 61:967-75. Abstract/Full Text
Proteins that bind high-mannose sugars of the HIV envelope.: Botos I, Wlodawer A.; Prog Biophys Mol Biol (2005 Jun) 88:233-82. Abstract/Full Text
Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains.: Rotanova TV, Melnikov EE, Khalatova AG, Makhovskaya OV, Botos I, Wlodawer A, Gustchina A.; Eur J Biochem (2004 Dec) 271:4865-71. Abstract/Full Text
Crystal structure of the AAA+ alpha domain of E. coli Lon protease at 1.9A resolution.: Botos I, Melnikov EE, Cherry S, Khalatova AG, Rasulova FS, Tropea JE, Maurizi MR, Rotanova TV, Gustchina A, Wlodawer A.; J Struct Biol (2004 Apr-May) 146:113-22. Abstract/Full Text
The catalytic domain of Escherichia coli Lon protease has a unique fold and a Ser-Lys dyad in the active site.: Botos I, Melnikov EE, Cherry S, Tropea JE, Khalatova AG, Rasulova F, Dauter Z, Maurizi MR, Rotanova TV, Wlodawer A, Gustchina A.; J Biol Chem (2004 Feb 27) 279:8140-8. Abstract/Full Text
Cyanovirin-N: a sugar-binding antiviral protein with a new twist.: Botos I, Wlodawer A.; Cell Mol Life Sci (2003 Feb) 60:277-87. Abstract/Full Text
Structures of the complexes of a potent anti-HIV protein cyanovirin-N and high mannose oligosaccharides.: Botos I, O'Keefe BR, Shenoy SR, Cartner LK, Ratner DM, Seeberger PH, Boyd MR, Wlodawer A.; J Biol Chem (2002 Sep 13) 277:34336-42. Abstract/Full Text
Domain-swapped structure of a mutant of cyanovirin-N.: Botos I, Mori T, Cartner LK, Boyd MR, Wlodawer A.; Biochem Biophys Res Commun (2002 May 31) 294:184-90. Abstract/Full Text
The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures.: Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM.; Structure (2002 May) 10:673-86. Abstract/Full Text
Crystal structures of the semireduced and inhibitor-bound forms of cyclic nucleotide phosphodiesterase from Arabidopsis thaliana.: Hofmann A, Grella M, Botos I, Filipowicz W, Wlodawer A.; J Biol Chem (2002 Jan 11) 277:1419-25. Abstract/Full Text
Crystal structure of a cyclic form of bovine pancreatic trypsin inhibitor.: Botos I, Wu Z, Lu W, Wlodawer A.; FEBS Lett (2001 Nov 30) 509:90-4. Abstract/Full Text
The structure of an insect chymotrypsin.: Botos I, Meyer E, Nguyen M, Swanson SM, Koomen JM, Russell DH, Meyer EF.; J Mol Biol (2000 May 19) 298:895-901. Abstract/Full Text
Structure of recombinant mouse collagenase-3 (MMP-13).: Botos I, Meyer E, Swanson SM, Lemaître V, Eeckhout Y, Meyer EF.; J Mol Biol (1999 Oct 1) 292:837-44. Abstract/Full Text
Batimastat, a potent matrix mealloproteinase inhibitor, exhibits an unexpected mode of binding.: Botos I, Scapozza L, Zhang D, Liotta LA, Meyer EF.; Proc Natl Acad Sci U S A (1996 Apr 2) 93:2749-54. Abstract/Full Text
Structure-based analysis of inhibitor binding to Ht-d.: Botos I, Scapozza L, Shannon JD, Fox JW, Meyer EF.; Acta Crystallogr D Biol Crystallogr (1995 Jul 1) 51:597-604. Abstract/Full Text
Structural interaction of natural and synthetic inhibitors with the venom metalloproteinase, atrolysin C (form d).: Zhang D, Botos I, Gomis-Rüth FX, Doll R, Blood C, Njoroge FG, Fox JW, Bode W, Meyer EF.; Proc Natl Acad Sci U S A (1994 Aug 30) 91:8447-51. Abstract/Full Text

Books & Book Chapters

"Toll-like receptors – Structure and signaling.": Botos I, Davies DR.; Handbook of Cell Signaling.; Bradshaw, ed.; Academic Press. (2009) 139-143.
"Snake venom metalloproteinases (SVMPs): Functional modulation of activity by non-proteinase domains and the structural relationship of these proteins to mammalian homologs.": Bjarnason JB, Botos I, Fox JW, Jia LG, Meyer EF.; The astacins. Zwilling R, Stocker W, eds. (1995) 349-361. Dr. Kovac, Hamburg.
Backwards binding and other structural surprises.: Botos I, Meyer EF, Scapozza L, Zhang D.; Perspect. Drug Discovery Des. (1995) 3:168-195.

Last Reviewed June 2023