Susan K. Buchanan, Ph.D.

Deputy Scientific Director: Division of Intramural Research
Chief: Laboratory of Molecular Biology
Section Chief: Structural Biology of Membrane Proteins Section, Laboratory of Molecular Biology

Scientific Focus Areas: Microbiology and Infectious Diseases, Molecular Biology and Biochemistry, Structural Biology

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Publications

A selection of recent and significant publications can be viewed below.

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Structural basis for iron piracy by pathogenic Neisseria.: Noinaj N, Easley NC, Oke M, Mizuno N, Gumbart J, Boura E, Steere AN, Zak O, Aisen P, Tajkhorshid E, Evans RW, Gorringe AR, Mason AB, Steven AC, Buchanan SK.; Nature (2012 Feb 12) 483:53-8. Abstract/Full Text
Structural insight into the biogenesis of β-barrel membrane proteins.: Noinaj N, Kuszak AJ, Gumbart JC, Lukacik P, Chang H, Easley NC, Lithgow T, Buchanan SK.; Nature (2013 Sep 19) 501:385-90. Abstract/Full Text
Structural insight into the role of the Ton complex in energy transduction.: Celia H, Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Barnard TJ, Cramer WA, Lloubes R, Buchanan SK.; Nature (2016 Oct 6) 538:60-65. Abstract/Full Text
Cryo-EM structure of the bacterial Ton motor subcomplex ExbB-ExbD provides information on structure and stoichiometry.: Celia H, Botos I, Ni X, Fox T, De Val N, Lloubes R, Jiang J, Buchanan SK.; Commun Biol (2019) 2:358. Abstract/Full Text
Structural insight into mitochondrial β-barrel outer membrane protein biogenesis.: Diederichs KA, Ni X, Rollauer SE, Botos I, Tan X, King MS, Kunji ERS, Jiang J, Buchanan SK.; Nat Commun (2020 Jul 3) 11:3290. Abstract/Full Text

Additional Publications

The ColM Family, Polymorphic Toxins Breaching the Bacterial Cell Wall.: Ghequire MGK, Buchanan SK, De Mot R.; mBio (2018 Feb 13) 9. Abstract/Full Text
Insertion of proteins and lipopolysaccharide into the bacterial outer membrane.: Botos I, Noinaj N, Buchanan SK.; Philos Trans R Soc Lond B Biol Sci (2017 Aug 5) 372. Abstract/Full Text
Structural snapshots of the β-barrel assembly machinery.: Bakelar J, Buchanan SK, Noinaj N.; FEBS J (2017 Jun) 284:1778-1786. Abstract/Full Text
Two-Partner Secretion: Combining Efficiency and Simplicity in the Secretion of Large Proteins for Bacteria-Host and Bacteria-Bacteria Interactions.: Guérin J, Bigot S, Schneider R, Buchanan SK, Jacob-Dubuisson F.; Front Cell Infect Microbiol (2017) 7:148. Abstract/Full Text
The β-barrel assembly machinery in motion.: Noinaj N, Gumbart JC, Buchanan SK.; Nat Rev Microbiol (2017 Apr) 15:197-204. Abstract/Full Text
A Natural Chimeric Pseudomonas Bacteriocin with Novel Pore-Forming Activity Parasitizes the Ferrichrome Transporter.: Ghequire MG, Kemland L, Anoz-Carbonell E, Buchanan SK, De Mot R.; mBio (2017 Feb 21) 8. Abstract/Full Text
Structure of the NPr:EIN(Ntr) Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems.: Strickland M, Stanley AM, Wang G, Botos I, Schwieters CD, Buchanan SK, Peterkofsky A, Tjandra N.; Structure (2016 Dec 6) 24:2127-2137. Abstract/Full Text
Structural Insights into Substrate Recognition and Catalysis in Outer Membrane Protein B (OmpB) by Protein-lysine Methyltransferases from Rickettsia.: Abeykoon AH, Noinaj N, Choi BE, Wise L, He Y, Chao CC, Wang G, Gucek M, Ching WM, Chock PB, Buchanan SK, Yang DC.; J Biol Chem (2016 Sep 16) 291:19962-74. Abstract/Full Text
From Constructs to Crystals - Towards Structure Determination of β-barrel Outer Membrane Proteins.: Noinaj N, Mayclin S, Stanley AM, Jao CC, Buchanan SK.; J Vis Exp (2016 Jul 4) Abstract/Full Text
Structural and Functional Characterization of the LPS Transporter LptDE from Gram-Negative Pathogens.: Botos I, Majdalani N, Mayclin SJ, McCarthy JG, Lundquist K, Wojtowicz D, Barnard TJ, Gumbart JC, Buchanan SK.; Structure (2016 Jun 7) 24:965-976. Abstract/Full Text
Beyond the Crystal Structure: Insight into the Function and Vaccine Potential of TbpA Expressed by Neisseria gonorrhoeae.: Cash DR, Noinaj N, Buchanan SK, Cornelissen CN.; Infect Immun (2015 Nov) 83:4438-49. Abstract/Full Text
Evidence of Distinct Channel Conformations and Substrate Binding Affinities for the Mitochondrial Outer Membrane Protein Translocase Pore Tom40.: Kuszak AJ, Jacobs D, Gurnev PA, Shiota T, Louis JM, Lithgow T, Bezrukov SM, Rostovtseva TK, Buchanan SK.; J Biol Chem (2015 Oct 23) 290:26204-17. Abstract/Full Text
Fitting the Pieces of the β-Barrel Assembly Machinery Complex.: O'Neil PK, Rollauer SE, Noinaj N, Buchanan SK.; Biochemistry (2015 Oct 20) 54:6303-11. Abstract/Full Text
Outer membrane protein biogenesis in Gram-negative bacteria.: Rollauer SE, Sooreshjani MA, Noinaj N, Buchanan SK.; Philos Trans R Soc Lond B Biol Sci (2015 Oct 5) 370. Abstract/Full Text
The β-barrel membrane protein insertase machinery from Gram-negative bacteria.: Noinaj N, Rollauer SE, Buchanan SK.; Curr Opin Struct Biol (2015 Apr) 31:35-42. Abstract/Full Text
Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria.: Noinaj N, Kuszak AJ, Buchanan SK.; Methods Mol Biol (2015) 1329:51-6. Abstract/Full Text
Methods to Characterize Folding and Function of BamA Cross-Link Mutants.: Kuszak AJ, Noinaj N, Buchanan SK.; Methods Mol Biol (2015) 1329:137-47. Abstract/Full Text
Summary and Future Directions.: Noinaj N, Buchanan SK.; Methods Mol Biol (2015) 1329:279-80. Abstract/Full Text
Reconstitution of a nanomachine driving the assembly of proteins into bacterial outer membranes.: Shen HH, Leyton DL, Shiota T, Belousoff MJ, Noinaj N, Lu J, Holt SA, Tan K, Selkrig J, Webb CT, Buchanan SK, Martin LL, Lithgow T.; Nat Commun (2014 Oct 24) 5:5078. Abstract/Full Text
Structural insights into the transport of small molecules across membranes.: Noinaj N, Buchanan SK.; Curr Opin Struct Biol (2014 Aug) 27:8-15. Abstract/Full Text
Lateral opening and exit pore formation are required for BamA function.: Noinaj N, Kuszak AJ, Balusek C, Gumbart JC, Buchanan SK.; Structure (2014 Jul 8) 22:1055-62. Abstract/Full Text
FhaC takes a bow to FHA in the two-partner do-si-do.: Noinaj N, Buchanan SK.; Mol Microbiol (2014 Jun) 92:1155-8. Abstract/Full Text
Structural insight into the lactoferrin receptors from pathogenic Neisseria.: Noinaj N, Cornelissen CN, Buchanan SK.; J Struct Biol (2013 Oct) 184:83-92. Abstract/Full Text
Using a bacteriocin structure to engineer a phage lysin that targets Yersinia pestis.: Lukacik P, Barnard TJ, Buchanan SK.; Biochem Soc Trans (2012 Dec 1) 40:1503-6. Abstract/Full Text
The transferrin-iron import system from pathogenic Neisseria species.: Noinaj N, Buchanan SK, Cornelissen CN.; Mol Microbiol (2012 Oct) 86:246-57. Abstract/Full Text
Dynamic association of BAM complex modules includes surface exposure of the lipoprotein BamC.: Webb CT, Selkrig J, Perry AJ, Noinaj N, Buchanan SK, Lithgow T.; J Mol Biol (2012 Sep 28) 422:545-55. Abstract/Full Text
Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis.: Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK.; Structure (2012 Jul 3) 20:1233-43. Abstract/Full Text
Molecular basis for the activation of a catalytic asparagine residue in a self-cleaving bacterial autotransporter.: Barnard TJ, Gumbart J, Peterson JH, Noinaj N, Easley NC, Dautin N, Kuszak AJ, Tajkhorshid E, Bernstein HD, Buchanan SK.; J Mol Biol (2012 Jan 6) 415:128-42. Abstract/Full Text
Structural insights into the catalytic mechanism of Escherichia coli selenophosphate synthetase.: Noinaj N, Wattanasak R, Lee DY, Wally JL, Piszczek G, Chock PB, Stadtman TC, Buchanan SK.; J Bacteriol (2012 Jan) 194:499-508. Abstract/Full Text
Role of the Yersinia pestis Ail protein in preventing a protective polymorphonuclear leukocyte response during bubonic plague.: Hinnebusch BJ, Jarrett CO, Callison JA, Gardner D, Buchanan SK, Plano GV.; Infect Immun (2011 Dec) 79:4984-9. Abstract/Full Text
Structural insights into Ail-mediated adhesion in Yersinia pestis.: Yamashita S, Lukacik P, Barnard TJ, Noinaj N, Felek S, Tsang TM, Krukonis ES, Hinnebusch BJ, Buchanan SK.; Structure (2011 Nov 9) 19:1672-82. Abstract/Full Text
The structural biology of β-barrel membrane proteins: a summary of recent reports.: Fairman JW, Noinaj N, Buchanan SK.; Curr Opin Struct Biol (2011 Aug) 21:523-31. Abstract/Full Text
The crystal structure and mechanism of an unusual oxidoreductase, GilR, involved in gilvocarcin V biosynthesis.: Noinaj N, Bosserman MA, Schickli MA, Piszczek G, Kharel MK, Pahari P, Buchanan SK, Rohr J.; J Biol Chem (2011 Jul 1) 286:23533-43. Abstract/Full Text
Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana.: Tong J, Dolezal P, Selkrig J, Crawford S, Simpson AG, Noinaj N, Buchanan SK, Gabriel K, Lithgow T.; Mol Biol Evol (2011 May) 28:1581-91. Abstract/Full Text
The crystal structure of BamB suggests interactions with BamA and its role within the BAM complex.: Noinaj N, Fairman JW, Buchanan SK.; J Mol Biol (2011 Mar 25) 407:248-60. Abstract/Full Text
A modular BAM complex in the outer membrane of the alpha-proteobacterium Caulobacter crescentus.: Anwari K, Poggio S, Perry A, Gatsos X, Ramarathinam SH, Williamson NA, Noinaj N, Buchanan S, Gabriel K, Purcell AW, Jacobs-Wagner C, Lithgow T.; PLoS One (2010 Jan 8) 5:e8619. Abstract/Full Text
TonB-dependent transporters: regulation, structure, and function.: Noinaj N, Guillier M, Barnard TJ, Buchanan SK.; Annu Rev Microbiol (2010) 64:43-60. Abstract/Full Text
Reconstitution of bacterial outer membrane TonB-dependent transporters in planar lipid bilayer membranes.: Udho E, Jakes KS, Buchanan SK, James KJ, Jiang X, Klebba PE, Finkelstein A.; Proc Natl Acad Sci U S A (2009 Dec 22) 106:21990-5. Abstract/Full Text
The protein import channel in the outer mitosomal membrane of Giardia intestinalis.: Dagley MJ, Dolezal P, Likic VA, Smid O, Purcell AW, Buchanan SK, Tachezy J, Lithgow T.; Mol Biol Evol (2009 Sep) 26:1941-7. Abstract/Full Text
Protein secretion and outer membrane assembly in Alphaproteobacteria.: Gatsos X, Perry AJ, Anwari K, Dolezal P, Wolynec PP, Likić VA, Purcell AW, Buchanan SK, Lithgow T.; FEMS Microbiol Rev (2008 Nov) 32:995-1009. Abstract/Full Text
Signaling mechanisms for activation of extracytoplasmic function (ECF) sigma factors.: Brooks BE, Buchanan SK.; Biochim Biophys Acta (2008 Sep) 1778:1930-45. Abstract/Full Text
Autotransporter structure reveals intra-barrel cleavage followed by conformational changes.: Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK.; Nat Struct Mol Biol (2007 Dec) 14:1214-20. Abstract/Full Text
A structural comparison of human serum transferrin and human lactoferrin.: Wally J, Buchanan SK.; Biometals (2007 Jun) 20:249-62. Abstract/Full Text
Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import.: Buchanan SK, Lukacik P, Grizot S, Ghirlando R, Ali MM, Barnard TJ, Jakes KS, Kienker PK, Esser L.; EMBO J (2007 May 16) 26:2594-604. Abstract/Full Text
Colicin biology.: Cascales E, Buchanan SK, Duché D, Kleanthous C, Lloubès R, Postle K, Riley M, Slatin S, Cavard D.; Microbiol Mol Biol Rev (2007 Mar) 71:158-229. Abstract/Full Text

Last Reviewed June 2024