NIDDK Workshop: Imaging the Pancreatic Beta Cell

Event Details

Meeting Objectives

This workshop is part of an ongoing program focused on in vivo imaging of islet mass, function, and disease pathogenesis that has been sponsored by the National Institutes of Health (NIH), Juvenile Diabetes Research Foundation (JDRF), and the European Union for more than a decade. The workshop will emphasize very cutting-edge beta cell biology that could inform the effort to visualize the beta cell, with an effort to highlight new data, reagents and ideas, new technologies that would be useful, lessons and reagents from neuroscience, and important imaging advances and recent work in islet/beta cell imaging.

Potential Topics

• What is a beta cell? Transdifferentiation, plasticity, and regeneration; new approaches to locating specific targets and targeting reagents
• The neuroscience of the islet: neurotransmitter receptors and pathways in the islet, catecholamines, neuroimaging reporters, and beta cell imaging
• Targeted functional biomarkers and targeted drug therapy
• Imaging cellular therapies to treat diabetes
• Imaging islet inflammation and beta cell stress: What causes diabetes? What keeps us from curing it?
• Imaging beta cell function and mass

Chairs

• Alejandro Caicedo (University of Miami)
• Markus Grompe (Oregon Health and Science University)
• Olle Korsgren (Uppsala University)
• Dean Sherry (University of Texas, Dallas and The University of Texas Southwestern Medical Center)

Organization Committee

• Olivier Blondel (NIDDK, NIH)
• Maren Laughlin (NIDDK, NIH)
• Antonio Sastre (NIBIB, NIH)
• Adrianne Wong (JDRF)

Program Committee

• Gary Cline (Yale University)
• Martin Gotthardt (Radboud University Nijmegen Medical Centre, the Netherlands)
• Paul Harris (Columbia University Medical College)
• Anna Moore (Massachusetts General Hospital)

Sponsors

• National Institute of Diabetes and Digestive and Kidney Diseases
• National Institute of Biomedical Imaging and Bioengineering
• Juvenile Diabetes Research Foundation

Agenda

April 15, 2013

8:00 a.m.

Welcome
Griffin Rodgers, M.D., M.A.C.P., Director, National Institute of Diabetes and Digestive and Kidney Diseases

8:10 a.m.

Keynote Address: Beta Cell Dedifferentiation and Plasticity in Diabetes
Domenico Accili

Session I: Inter-islet Communication: Nerves, Neurotransmitters, Vasculature, and Cell-cell Interactions

8:40 a.m.

Keynote: Role of Nerves in the Islet
Alejandro Caicedo

9:10 a.m.

Discussion (with leader)

9:20 a.m.

Invited Talk: Autocrine and Paracrine Regulation of Islet Cell Function
Patrik Rorsman

9:50 a.m.

Discussion (with leader)

10:00 a.m.

Break

10:20 a.m.

Invited Talk: Cell-cell and Vascular-cell Interactions in the Islet
Eckhard Lammert

10:50 a.m.

Discussion (with leader)

11:00 a.m.

Submitted Talk: CT Perfusion Monitoring of the Pancreas in Streptozotocin-induced Diabetic Rats
Joo Ho Tai

11:15 a.m.

Submitted Talk: Gadolinium-enhanced MRI of the Pancreatic Extracellular Volume to Detect Immune Infiltration Preceding Development of Type 1 Diabetes Mellitus in Mice
Patrick F. Antkowiak

11:30 a.m.

Submitted Talk: Quantification of Pancreatic VMAT2 Binding Using (18F)FP-(+)-DTBZ in Human PET Studies: Optimization of Methods
Mika Naganawa

11:45 a.m.

Submitted Talk: Functional Role of Dopamine Type 2 Receptors (D2R) and Vesicular Monoamine Transporter Type 2 (VMAT2) in Glucose Stimulated Insulin Secretion (GSIS) by Human Islets
Paul Harris

12:05 p.m.

European Efforts, European Association for the Study of Diabetes Metabolic Imaging Study Group
Martin Gotthardt

12:15 p.m.

Lunch (on your own) and Poster Session

There will be two informal, open discussions starting at 2:15 p.m., which will be held among the posters.

1. DTBZ Ligands and the VMAT2 Target
2. GLP-1 Receptor Targeting

Session II: Imaging Islet Function and Mass

3:00 p.m.

Chair's Introduction: Zn imaging of Insulin Secretion
Dean Sherry

3:20 p.m.

Keynote: Challenges and Opportunities in Beta Cell Imaging
Al Powers

3:50 p.m.

Discussion (with leader)

4:00 p.m.

Invited Talk
Guy Rutter

4:20 p.m.

Discussion (with leader)

4:30 p.m.

Break

4:50 p.m.

Invited Talk: Manganese-enhanced Magnetic Resonance Imaging (MEMRI) of Pancreatic Islets, and Multivalent Targeting of Beta Cells
Paolo Meda

5:20 p.m.

Discussion (with leader)

5:30 p.m.

Submitted Talk: Quantitative Pathophysiological Analyses of the Whole Human Pancreas
Manami Hara

5:45 p.m.

Submitted Talk: Introducing a Novel FRET Biosensor for Glycolytic Activity: Islet Beta Cell Glycolytic and Mitochondial Function Measured Simultaneously and in Real Time
Matthew J. Merrins

6:00 p.m.

Submitted Talk: Application of a Novel GLP-1 Analogue for GLP-1R Targeted Imaging in Ossabaw Mini Pigs: Initial Result
William Silvers

6:15 p.m.

Submitted Talk: A Heterobivalent Ligand Comprised of GLP-1 and Yohimbine Specifically Targets Pancreatic β-Cells In Vivo
Sean W. Limesand

6:30 p.m.

Adjournment

April 16, 2013

8:00 a.m.

Industry Perspective: Imaging Biomarker Needs for Preclinical Development and Clinical Trials in Diabetes
Melissa Thomas, Lily Pharmaceuticals

Session III: Beta Cell Markers and Search for Targeting Agents

8:20 a.m.

Chair's Introduction
Markus Grompe

8:30 a.m.

Keynote: Clinical and Practical Applications of Beta-cell Imaging
David Harlan

9:00 a.m.

Discussion (with leader)

9:10 a.m.

Invited Talk: Toward Islet Cell Specific Reagents
Phil Streeter

9:40 a.m.

Discussion (with leader)

9:50 a.m.

Break

10:10 a.m.

Invited Talk: Radiopharmaceutical Development
Mark Mintun, Avid Radiopharmaceuticals

10:30 a.m.

Discussion (with leader)

10:40 a.m.

Submitted Talk: Exendin-4 Loaded Magnetic Iron Nanoparticles for Pancreatic Islet Imaging
Ping Wang

10:55 a.m.

Submitted Talk: Non-invasive Determination of the Beta Cell Mass by SPECT With 111In-DTPA-exendin-3 in a Rat Model for Spontaneous Type 1 Diabetes
Maarten Brom

11:10 a.m.

Submitted Talk: Preparation of Beta-cell Targeted Magnetic Nanoparticle With Sphingomyelin Specificity
Amol Kavishwar

11:25 a.m.

Submitted Talk: Imaging With Aptamers
Giacomo Lanzoni

11:40 a.m.

EC Perspective
Nathalie Vercruysse

11:50 a.m.

Lunch and Poster Session

Session IV: Transplantation and Immunology, Including Cell Therapies

1:30 p.m.

Chair's Introduction
Olle Korsgren

1:40 p.m.

Keynote: Imaging Beta Cell Mass
Olof Eriksson

2:10 p.m.

Discussion (with leader)

2:20 p.m.

Invited Talk: Imaging the T1D Pancreas: Anatomy, Beta Cell Mass, and Inflammation
Jason Gaglia

2:50 p.m.

Discussion (with leader)

3:00 p.m.

Break

3:20 p.m.

Invited Talk: Insulitis and Islet Volume in the Non-obese Diabetic (NOD) Mouse
Dan Holmberg

3:50 p.m.

Discussion (with leader)

4:00 p.m.

Submitted Talk: High-resolution In Vivo Imaging of Transplanted Pancreatic Islets Reveals Complex Behavior of Innate Immune Cells in Engraftment
Gustaf Christoffersson

4:15 p.m.

Submitted Talk: Dynamic Contrast Enhanced-MRI (DCE-MRI) Functional Assessment of Vascular Events Occurring During the Intra-hepatic Engraftment of Pancreatic Islets May Predict Long-term Outcome of Islets Transplantation
Anna Palmisano

4:30 p.m.

Submitted Talk: Effect of Mesenchymal Stem Cells on the Vascularization of the Artificial Cavity Used as a Site for Islet Transplantation
Daniel Jirak

4:45 p.m.

Summation and Discussion
Chairs

5:00 p.m.

Adjournment