10th Annual Course on Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis
The tenth annual course provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in humans and in animals. The course is designed for beginners as well as those with experience who wish to expand their capabilities to more sophisticated problems. The faculty is well-versed in a variety of applications and methodologies. Techniques will be presented for investigating whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation. The basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Course material will be available for download from http://www.mmpc.org/shared/tracers.aspx.
For more information regarding this meeting, please visit the official meeting page.
Problems and discussion questions will highlight key concepts. In addition to organized sessions, individual attendees will have ample opportunities for personal interaction with faculty members in the form of one-on-one mentoring sessions to discuss their research projects in more depth.
Henri Brunengraber*, Case Western Reserve University
Gary Cline, Yale University
Melanie Cree Green, University of Colorado
Joanne Kelleher, Mass. Inst. Tech.
Maren Laughlin, National Inst. Health
Owen McGuinness*, Vanderbilt University
Matthew Merritt, University of Florida
Elizabeth Parks, University of Missouri
Stephen Previs, Merck
Michelle Puchowicz, Case Western Reserve University
Jamey Young, Vanderbilt University
Robert R. Wolfe*, University Arkansas
September 30, 2017
Minutes are currently unavailable.
Attendees are currently unavailable.