Beyond Transcriptomics: Understanding Erythrocyte Maturation

Event Details

Background

Prior to nuclear extrusion from pronormoblasts, critical pathways are developed and continue functioning to ensure optimal erythrocyte function. Knowledge about individual gene products does not necessarily inform us about pathways/processes and their interactions. The purpose of this workshop is to explore these processes and to discuss metabolic reprogramming, proteostasis, integration of transcriptomics, proteomics and other -omics, and enucleation. Please join us in an open forum to discuss the state of the field and to identify gaps in our knowledge.

Organizing Committee

External Organizers

David Bodine, Chief, Hematopoiesis Section, NHGRI
Stuart Orkin, M.D., Boston Children’s Hospital
Vijay Sankaran, M.D./Ph.D., Harvard Medical School
Mitchell Weiss, M.D./Ph.D., St. Jude Children’s Research Hospital
Yang Xia, M.D./Ph.D., University of Texas Medical School at Houston

NIH Organizers

Terry Rogers Bishop, Ph.D., NIDDK, Division of Kidney, Urologic, and Hematologic Diseases
Rebecca Fuldner, Ph.D., NIA, Division of Aging Biology
Malgorzata (Gosia) Klauzinska, NHLBI, Molecular, Cellular & Systems Blood Science Branch
Cindy Roy, Ph.D., NIDDK, Division of Kidney, Urologic, and Hematologic Diseases
John W. Thomas, Ph.D., NHLBI, Molecular, Cellular & Systems Blood Science Branch
John P. Williams, Ph.D., NIA, Division of Aging Biology

Registration Deadline

May 21, 2018

Agenda

May 29, 2018

7:30 a.m. – 8:00 a.m.

Registration and Poster Set-up

8:00 a.m. – 8:15 a.m.

Welcome and Opening Remarks
Griffin P. Rodgers, M.D., MACP, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH)

8:15 a.m. – 8:30 a.m.

Introductions and Goals of the Workshop
Terry R. Bishop, Ph.D., NIDDK, NIH

Session 1: Metabolic Reprogramming

8:30 a.m. – 9:15 a.m.

Probing “developmental metabolism” for clues to disorders
Utpal Banerjee, Ph.D., University of California, Los Angeles

9:15 a.m. – 9:45 a.m.

Krebs cycle intermediates: Regulators of erythroid diferentation
Naomi Taylor, M.D., Ph.D., Institute of Molecular Genetics of Montpellier, INSERM

9:45 a.m. – 10:15 a.m.

Erythrocyte Hypoxic Metabolic Reprogramming, Adaptation, Memory and Sickle Cell Disease
Yang Xia, M.D., Ph.D., The University of Texas Health Science Center at Houston

10:15 a.m. – 10:30 a.m.

Break

Session 2: Integration of “-omics”

10:30 a.m. – 11:00 a.m.

The Regulation of Translation in Erythropoiesis
Vijay Sankaran, M.D., Ph.D., Harvard Stem Cell Institute, Harvard University

11:00 a.m. – 11:30 a.m.

Integrative Proteomic and Transcriptomic Analyses Uncover New Mechanisms of Mitochondrial Biogenesis during Erythropoiesis
Jian Xu, Ph.D., The University of Texas Southwestern Medical Center

11:30 a.m. – 1:30 p.m.

Lunch and Poster Presentations

Session 2 (cont.): Integration of “-omics”

1:30 p.m. – 2:00 p.m.

Understanding Erythropoiesis Using Quantitative Proteomics and Single-cell Mass Cytometry
Marjorie Brand, Ph.D., Ottawa Hospital Research Institute, University of Ottawa

Session 3: Proteostasis

2:00 p.m. – 2:30 p.m.

The Biochemical Program of Extreme Terminal Differentiation
Daniel Finley, Ph.D., Harvard Medical School, Harvard University

2:30 p.m. – 3:00 p.m.

Mapping the Erythrocyte Interactome by Deep Proteomics
Edward Marcotte, Ph.D., The University of Texas, Austin

Session 4: Enucleation

3:00 p.m. – 3:30 p.m.

Erythroblast Maturation – Cytoskeletal and Ontogenic Considerations
James Palis, M.D., University of Rochester Medical Center

3:30 p.m. – 3:45 p.m.

Break

3:45 p.m. – 4:15 p.m.

A Super-resolution Window into Hematology
Ke Xu, Ph.D., University of California, Berkeley

4:15 p.m. – 5:30 p.m.

Summary and Discussion to Identify Gaps in Knowledge
Stuart Orkin, M.D., Boston Children’s Hospital, Harvard University, Moderator
Mitchell Weiss, M.D., Ph.D., St. Jude’s Children’s Hospital, Moderator

5:30 p.m.

Adjournment

Abstracts

Submission Deadline

May 7, 2018

Submitting Abstracts

All abstracts must be submitted via email to Mr. John Hare of The Scientific Consulting Group, Inc. with “Beyond Transcriptomics” in the subject line. The body of the email should identify the submitting author and the career development stage of the submitting author: student, fellow, instructor, assistant professor/scientist, etc.

Selected abstracts will be presented in poster form.

Junior-level participants (students, fellows, instructors, and assistant professors) with the highest-scoring abstracts will be eligible for a modest travel award.

All abstracts will be accepted for poster presentation. Those selected for travel awards will be notified the week of May 21.

Formatting Requirements

1. A completed poster abstract should include a statement of purpose, methods used, and a summary of results in sufficient detail to support the conclusion. Statements such as “results will be described” should be avoided.
2. The poster abstract should fit on half of an 8.5" × 11" page. Abstracts should be no longer than 250 words (not including name and affiliation).
3. The abstract should be typed single-spaced using 12-point Times New Roman font.
4. The title should be boldface and followed by one return.
5. The author list (authors’ first and last names, degrees) with institutions listed in a separate paragraph—including city, state, and country—should be followed by one return. The name of the presenting author should be underlined. Provide any grant acknowledgments for your research (e.g., R01-DK123456) at the end of your abstract.
6. The abstract file should be saved as: presenting author’s Last name_First word in the title (e.g., Zucker_Effects).

Poster Presentations

Selected poster abstracts will appear in the meeting materials. Each author is allotted a 4-foot-high by 6-foot-wide poster board. The poster session will be held during the lunch break.

If your abstract is selected for a poster presentation, please arrive at 7:30a.m., May 29, 2018, to mount your poster on the posterboard. Pushpins and Velcro will be provided onsite. You will be responsible for removing and shipping your poster at the end of the meeting. Any remaining posters will be discarded.

If you have any questions about the scientific content of the workshop, please contact Dr. Terry Bishop. For questions/concerns about workshop logistics, please contact Mr. John Hare. We look forward to meeting and working with you. We anticipate a stimulating and productive workshop.

Photography Guidelines

Photography is not allowed where posters are displayed.