13th Annual Course on Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis
A week-long course (with one-on-one mentoring sessions) in the theory and practice of stable and radioactive isotopic tracers for the study of metabolism in human and animal models.
This course provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes in humans, animals and cells. The course is designed for beginners as well as for those with experience who wish to expand their capabilities to more sophisticated problems. The faculty has expertise in a wide variety of applications and methodologies.
Techniques are taught for the investigation of whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation in humans, animals and cells. Basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR are discussed, including examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Attendees will have opportunities to discuss their research projects in one-on-one mentoring sessions with faculty.
Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)
Course Co-Directors: Owen P. McGuinness, PhD, Elizabeth J. Parks, PhD
Rolling waitlist after August 13, 2021