Nicholas R. Guydosh, Ph.D.
Nicholas Guydosh
Scientific Focus Areas: Biomedical Engineering and Biophysics, Computational Biology, Genetics and Genomics, Molecular Biology and Biochemistry, RNA Biology


A selection of recent and significant publications can be viewed below.

Select Publications

40S ribosome profiling reveals distinct roles for Tma20/Tma22 (MCT-1/DENR) and Tma64 (eIF2D) in 40S subunit recycling.
Young DJ, Meydan S, Guydosh NR.
Nat Commun (2021 May 20) 12:2976. Abstract/Full Text
The ubiquitin conjugase Rad6 mediates ribosome pausing during oxidative stress.
Meydan S, Barros GC, Simões V, Harley L, Cizubu BK, Guydosh NR, Silva GM.
Cell Rep (2023 Nov 28) 42:113359. Abstract/Full Text
Disome and Trisome Profiling Reveal Genome-wide Targets of Ribosome Quality Control.
Meydan S, Guydosh NR.
Mol Cell (2020 Aug 20) 79:588-602.e6. Abstract/Full Text
Hcr1/eIF3j Is a 60S Ribosomal Subunit Recycling Accessory Factor In Vivo.
Young DJ, Guydosh NR.
Cell Rep (2019 Jul 2) 28:39-50.e4. Abstract/Full Text
Activation of the antiviral factor RNase L triggers translation of non-coding mRNA sequences.
Karasik A, Jones GD, DePass AV, Guydosh NR.
Nucleic Acids Res (2021 Jun 21) 49:6007-6026. Abstract/Full Text

Additional Publications

Is there a localized role for translational quality control?
Meydan S, Guydosh NR.
RNA (2023 Nov) 29:1623-1643. Abstract/Full Text
Endonucleolytic RNA cleavage drives changes in gene expression during the innate immune response.
Karasik A, Lorenzi HA, DePass AV, Guydosh NR.
bioRxiv (2023 Oct 1) Abstract/Full Text
mRNA location and translation rate determine protein targeting to dual destinations.
Gasparski AN, Moissoglu K, Pallikkuth S, Meydan S, Guydosh NR, Mili S.
Mol Cell (2023 Aug 3) 83:2726-2738.e9. Abstract/Full Text
Rebirth of the translational machinery: The importance of recycling ribosomes.
Young DJ, Guydosh NR.
Bioessays (2022 Apr) 44:e2100269. Abstract/Full Text
Context-specific action of macrolide antibiotics on the eukaryotic ribosome.
Svetlov MS, Koller TO, Meydan S, Shankar V, Klepacki D, Polacek N, Guydosh NR, Vázquez-Laslop N, Wilson DN, Mankin AS.
Nat Commun (2021 May 14) 12:2803. Abstract/Full Text
A cellular handbook for collided ribosomes: surveillance pathways and collision types.
Meydan S, Guydosh NR.
Curr Genet (2021 Feb) 67:19-26. Abstract/Full Text
UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis.
Wang L, Xu Y, Rogers H, Saidi L, Noguchi CT, Li H, Yewdell JW, Guydosh NR, Ye Y.
Cell Res (2020 Jan) 30:5-20. Abstract/Full Text
Tma64/eIF2D, Tma20/MCT-1, and Tma22/DENR Recycle Post-termination 40S Subunits In Vivo.
Young DJ, Makeeva DS, Zhang F, Anisimova AS, Stolboushkina EA, Ghobakhlou F, Shatsky IN, Dmitriev SE, Hinnebusch AG, Guydosh NR.
Mol Cell (2018 Sep 6) 71:761-774.e5. Abstract/Full Text
Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe.
Guydosh NR, Kimmig P, Walter P, Green R.
Elife (2017 Sep 25) 6. Abstract/Full Text
IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts.
de Vasconcellos JF, Tumburu L, Byrnes C, Lee YT, Xu PC, Li M, Rabel A, Clarke BA, Guydosh NR, Proia RL, Miller JL.
Proc Natl Acad Sci U S A (2017 Jul 11) 114:E5664-E5672. Abstract/Full Text
Translation of poly(A) tails leads to precise mRNA cleavage.
Guydosh NR, Green R.
RNA (2017 May) 23:749-761. Abstract/Full Text
Silence without stress.
Young DJ, Guydosh NR.
Elife (2016 Nov 11) 5. Abstract/Full Text
Rli1/ABCE1 Recycles Terminating Ribosomes and Controls Translation Reinitiation in 3'UTRs In Vivo.
Young DJ, Guydosh NR, Zhang F, Hinnebusch AG, Green R.
Cell (2015 Aug 13) 162:872-84. Abstract/Full Text
Examining kinesin processivity within a general gating framework.
Andreasson JO, Milic B, Chen GY, Guydosh NR, Hancock WO, Block SM.
Elife (2015 Apr 22) 4. Abstract/Full Text
High-precision analysis of translational pausing by ribosome profiling in bacteria lacking EFP.
Woolstenhulme CJ, Guydosh NR, Green R, Buskirk AR.
Cell Rep (2015 Apr 7) 11:13-21. Abstract/Full Text
Dom34 rescues ribosomes in 3' untranslated regions.
Guydosh NR, Green R.
Cell (2014 Feb 27) 156:950-62. Abstract/Full Text
Nascent peptides that block protein synthesis in bacteria.
Woolstenhulme CJ, Parajuli S, Healey DW, Valverde DP, Petersen EN, Starosta AL, Guydosh NR, Johnson WE, Wilson DN, Buskirk AR.
Proc Natl Acad Sci U S A (2013 Mar 5) 110:E878-87. Abstract/Full Text
Direct observation of the binding state of the kinesin head to the microtubule.
Guydosh NR, Block SM.
Nature (2009 Sep 3) 461:125-8. Abstract/Full Text
Precision steering of an optical trap by electro-optic deflection.
Valentine MT, Guydosh NR, Gutiérrez-Medina B, Fehr AN, Andreasson JO, Block SM.
Opt Lett (2008 Mar 15) 33:599-601. Abstract/Full Text
Not so lame after all: kinesin still walks with a hobbled head.
Guydosh NR, Block SM.
J Gen Physiol (2007 Nov) 130:441-4. Abstract/Full Text
Molecule by molecule, the physics and chemistry of life: SMB 2007.
Block SM, Larson MH, Greenleaf WJ, Herbert KM, Guydosh NR, Anthony PC.
Nat Chem Biol (2007 Apr) 3:193-7. Abstract/Full Text
Backsteps induced by nucleotide analogs suggest the front head of kinesin is gated by strain.
Guydosh NR, Block SM.
Proc Natl Acad Sci U S A (2006 May 23) 103:8054-9. Abstract/Full Text
Unifying features in protein-folding mechanisms.
Gianni S, Guydosh NR, Khan F, Caldas TD, Mayor U, White GW, DeMarco ML, Daggett V, Fersht AR.
Proc Natl Acad Sci U S A (2003 Nov 11) 100:13286-91. Abstract/Full Text
The complete folding pathway of a protein from nanoseconds to microseconds.
Mayor U, Guydosh NR, Johnson CM, Grossmann JG, Sato S, Jas GS, Freund SM, Alonso DO, Daggett V, Fersht AR.
Nature (2003 Feb 20) 421:863-7. Abstract/Full Text
Last Reviewed January 2024