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Mini Mu containing plasmid and a method for rapid DNA sequencing (U.S. Patent Number 4,716,105)

The present invention discloses a rapid method of sequencing a relatively large segment of deoxyribonucleic acid. The method in part comprises high frequency insertion of a suitable transposon into a segment of DNA of interest. Preferable use of Mu transposons is described. A plasmid having mini-Mu transposons has been prepared and disclosed.

Resource Details

Resource Link: http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PALL&p=1&u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&r=1&f=G&l=50&s1=4,716,105.PN.&OS=PN/4,716,105&RS=PN/4,716,105

NIDDK Contact: tao@niddk.nih.gov

Principal Investigator(s): Mizuuchi, Kiyoshi