MUP-tTA Mouse

Tetracycline-responsive transcriptional activator driven by the liver-specific mouse major urinary protein promoter (MUP-tTA).

The E. Coli tetracycline operon regulatory system was used to generate a liver-specific transcription activation system that was inhibited by tetracycline. The transcription activator was a fused protein consisting of a tetracycline repressor gene (tetR) that was only active in the presence of tetracycline and a herpes simplex virus protein (VP-16) transcription activating domain (Tet-Off). Transcription was induced only in the absence of tetracycline (Tet-Off). A liver-specific promoter such as the mouse major urinary protein (MUP) promoter determined that the tetracycline-regulated transcriptional activator (tTA) would be expressed specifically in liver. To study the effect of the transcription activator on a target gene (for example, beta-galactosidase, LacZ) specifically in liver, MUP-tTA mice would be mated with transgenic mice in which the TAg Target gene was controlled by the E.Coli Tetracycline Operator (Tet-O).

Publication

Conditional liver-specific expression of simian virus 40 T antigen leads to regulatable development of hepatic neoplasm in transgenic mice.
Manickan E, Satoi J, Wang TC, Liang TJ.
J Biol Chem (2001 Apr) 276(17):13989-94. Abstract/Full Text

Resource Details

https://www.techtransfer.nih.gov/tech/tab-2421

tao@niddk.nih.gov

Liang, T. Jake

E-126-2012-0

Mutant Mouse: Transgene expresses the tetracycline-inhibitable transcription factor driven by the mouse major urinary protein promoter

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Last Reviewed October 2024