Harris D. Bernstein, Ph.D.

Photo of Harris Bernstein
Scientific Focus Areas: Microbiology and Infectious Diseases, Molecular Biology and Biochemistry


A selection of recent and significant publications can be viewed below.

Select Publications

Mechanistic link between β barrel assembly and the initiation of autotransporter secretion.
Pavlova O, Peterson JH, Ieva R, Bernstein HD.
Proc Natl Acad Sci U S A (2013 Mar 5) 110:E938-47. Abstract/Full Text
Sequential and spatially restricted interactions of assembly factors with an autotransporter beta domain.
Ieva R, Tian P, Peterson JH, Bernstein HD.
Proc Natl Acad Sci U S A (2011 Aug 2) 108:E383-91. Abstract/Full Text

Additional Publications

Mutations in the Escherichia coli ribosomal protein L22 selectively suppress the expression of a secreted bacterial virulence factor.
Yap MN, Bernstein HD.
J Bacteriol (2013 Jul) 195:2991-9. Abstract/Full Text
Cell biology: All clear for ribosome landing.
Bernstein HD.
Nature (2012 Dec 13) 492:189-91. Abstract/Full Text
Molecular basis for the activation of a catalytic asparagine residue in a self-cleaving bacterial autotransporter.
Barnard TJ, Gumbart J, Peterson JH, Noinaj N, Easley NC, Dautin N, Kuszak AJ, Tajkhorshid E, Bernstein HD, Buchanan SK.
J Mol Biol (2012 Jan 6) 415:128-42. Abstract/Full Text
Residues in a conserved α-helical segment are required for cleavage but not secretion of an Escherichia coli serine protease autotransporter passenger domain.
Dautin N, Bernstein HD.
J Bacteriol (2011 Aug) 193:3748-56. Abstract/Full Text
The translational regulatory function of SecM requires the precise timing of membrane targeting.
Yap MN, Bernstein HD.
Mol Microbiol (2011 Jul) 81:540-53. Abstract/Full Text
The double life of a bacterial lipoprotein.
Bernstein HD.
Mol Microbiol (2011 Mar) 79:1128-31. Abstract/Full Text
Secretion of a bacterial virulence factor is driven by the folding of a C-terminal segment.
Peterson JH, Tian P, Ieva R, Dautin N, Bernstein HD.
Proc Natl Acad Sci U S A (2010 Oct 12) 107:17739-44. Abstract/Full Text
The conformation of a nascent polypeptide inside the ribosome tunnel affects protein targeting and protein folding.
Peterson JH, Woolhead CA, Bernstein HD.
Mol Microbiol (2010 Oct) 78:203-17. Abstract/Full Text
Molecular basis for the structural stability of an enclosed β-barrel loop.
Tian P, Bernstein HD.
J Mol Biol (2010 Sep 17) 402:475-89. Abstract/Full Text
Type V Secretion: the Autotransporter and Two-Partner Secretion Pathways.
Bernstein HD.
EcoSal Plus (2010 Sep) 4. Abstract/Full Text
Sequential translocation of an Escherchia coli two-partner secretion pathway exoprotein across the inner and outer membranes.
Choi PS, Bernstein HD.
Mol Microbiol (2010 Jan) 75:440-51. Abstract/Full Text
Interaction of an autotransporter passenger domain with BamA during its translocation across the bacterial outer membrane.
Ieva R, Bernstein HD.
Proc Natl Acad Sci U S A (2009 Nov 10) 106:19120-5. Abstract/Full Text
Identification of a post-targeting step required for efficient cotranslational translocation of proteins across the Escherichia coli inner membrane.
Tian P, Bernstein HD.
J Biol Chem (2009 Apr 24) 284:11396-404. Abstract/Full Text
The plasticity of a translation arrest motif yields insights into nascent polypeptide recognition inside the ribosome tunnel.
Yap MN, Bernstein HD.
Mol Cell (2009 Apr 24) 34:201-11. Abstract/Full Text
Incorporation of a polypeptide segment into the beta-domain pore during the assembly of a bacterial autotransporter.
Ieva R, Skillman KM, Bernstein HD.
Mol Microbiol (2008 Jan) 67:188-201. Abstract/Full Text
Autotransporter structure reveals intra-barrel cleavage followed by conformational changes.
Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK.
Nat Struct Mol Biol (2007 Dec) 14:1214-20. Abstract/Full Text
Are bacterial 'autotransporters' really transporters?
Bernstein HD.
Trends Microbiol (2007 Oct) 15:441-7. Abstract/Full Text
Characterization of a novel two-partner secretion system in Escherichia coli O157:H7.
Choi PS, Dawson AJ, Bernstein HD.
J Bacteriol (2007 May) 189:3452-61. Abstract/Full Text
Cleavage of a bacterial autotransporter by an evolutionarily convergent autocatalytic mechanism.
Dautin N, Barnard TJ, Anderson DE, Bernstein HD.
EMBO J (2007 Apr 4) 26:1942-52. Abstract/Full Text
Protein secretion in gram-negative bacteria via the autotransporter pathway.
Dautin N, Bernstein HD.
Annu Rev Microbiol (2007) 61:89-112. Abstract/Full Text
The surprising complexity of signal sequences.
Hegde RS, Bernstein HD.
Trends Biochem Sci (2006 Oct) 31:563-71. Abstract/Full Text
Translation arrest requires two-way communication between a nascent polypeptide and the ribosome.
Woolhead CA, Johnson AE, Bernstein HD.
Mol Cell (2006 Jun 9) 22:587-98. Abstract/Full Text
An unusual signal peptide extension inhibits the binding of bacterial presecretory proteins to the signal recognition particle, trigger factor, and the SecYEG complex.
Peterson JH, Szabady RL, Bernstein HD.
J Biol Chem (2006 Apr 7) 281:9038-48. Abstract/Full Text
Efficient secretion of a folded protein domain by a monomeric bacterial autotransporter.
Skillman KM, Barnard TJ, Peterson JH, Ghirlando R, Bernstein HD.
Mol Microbiol (2005 Nov) 58:945-58. Abstract/Full Text
An unusual signal peptide facilitates late steps in the biogenesis of a bacterial autotransporter.
Szabady RL, Peterson JH, Skillman KM, Bernstein HD.
Proc Natl Acad Sci U S A (2005 Jan 4) 102:221-6. Abstract/Full Text
Neonatal natural killer cells produce chemokines and suppress HIV replication in vitro.
Bernstein HB, Kinter AL, Jackson R, Fauci AS.
AIDS Res Hum Retroviruses (2004 Nov) 20:1189-95. Abstract/Full Text
Conserved tertiary base pairing ensures proper RNA folding and efficient assembly of the signal recognition particle Alu domain.
Huck L, Scherrer A, Terzi L, Johnson AE, Bernstein HD, Cusack S, Weichenrieder O, Strub K.
Nucleic Acids Res (2004) 32:4915-24. Abstract/Full Text
Basic amino acids in a distinct subset of signal peptides promote interaction with the signal recognition particle.
Peterson JH, Woolhead CA, Bernstein HD.
J Biol Chem (2003 Nov 14) 278:46155-62. Abstract/Full Text
Signal recognition particle (SRP)-mediated targeting and Sec-dependent translocation of an extracellular Escherichia coli protein.
Sijbrandi R, Urbanus ML, ten Hagen-Jongman CM, Bernstein HD, Oudega B, Otto BR, Luirink J.
J Biol Chem (2003 Feb 14) 278:4654-9. Abstract/Full Text
Trigger factor retards protein export in Escherichia coli.
Lee HC, Bernstein HD.
J Biol Chem (2002 Nov 8) 277:43527-35. Abstract/Full Text