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Harris D. Bernstein, Ph.D.
Harris Bernstein.
Section Chief: Protein Biogenesis Section, Genetics & Biochemistry Branch
Scientific Focus Areas: Microbiology and Infectious Diseases, Molecular Biology and Biochemistry

Publications

A selection of recent and significant publications can be viewed below.

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The translocation assembly module (TAM) catalyzes the assembly of bacterial outer membrane proteins in vitro.
Wang X, Nyenhuis SB, Bernstein HD.
Nat Commun (2024 Aug 23) 15:7246. Abstract/Full Text
Cryo-EM structures reveal multiple stages of bacterial outer membrane protein folding.
Doyle MT, Jimah JR, Dowdy T, Ohlemacher SI, Larion M, Hinshaw JE, Bernstein HD.
Cell (2022 Mar 31) 185:1143-1156.e13. Abstract/Full Text
BamA forms a translocation channel for polypeptide export across the bacterial outer membrane.
Doyle MT, Bernstein HD.
Mol Cell (2021 May 6) 81:2000-2012.e3. Abstract/Full Text
Bacterial outer membrane proteins assemble via asymmetric interactions with the BamA β-barrel.
Doyle MT, Bernstein HD.
Nat Commun (2019 Jul 26) 10:3358. Abstract/Full Text
Folding of a bacterial integral outer membrane protein is initiated in the periplasm.
Sikdar R, Peterson JH, Anderson DE, Bernstein HD.
Nat Commun (2017 Nov 3) 8:1309. Abstract/Full Text

Additional Publications

Small Molecule Antibiotics Inhibit Distinct Stages of Bacterial Outer Membrane Protein Assembly.
Peterson JH, Doyle MT, Bernstein HD.
mBio (2022 Oct 26) 13:e0228622. Abstract/Full Text
Function of the Omp85 Superfamily of Outer Membrane Protein Assembly Factors and Polypeptide Transporters.
Doyle MT, Bernstein HD.
Annu Rev Microbiol (2022 Sep 8) 76:259-279. Abstract/Full Text
Reconstitution of Bam Complex-Mediated Assembly of a Trimeric Porin into Proteoliposomes.
Hussain S, Peterson JH, Bernstein HD.
mBio (2021 Aug 31) 12:e0169621. Abstract/Full Text
Bacterial Outer Membrane Proteins Are Targeted to the Bam Complex by Two Parallel Mechanisms.
Wang X, Peterson JH, Bernstein HD.
mBio (2021 May 4) 12. Abstract/Full Text
The Bam complex catalyzes efficient insertion of bacterial outer membrane proteins into membrane vesicles of variable lipid composition.
Hussain S, Bernstein HD.
J Biol Chem (2018 Feb 23) 293:2959-2973. Abstract/Full Text
Selective pressure for rapid membrane integration constrains the sequence of bacterial outer membrane proteins.
Peterson JH, Plummer AM, Fleming KG, Bernstein HD.
Mol Microbiol (2017 Dec) 106:777-792. Abstract/Full Text
Genomic Diversity of Enterotoxigenic Strains of Bacteroides fragilis.
Pierce JV, Bernstein HD.
PLoS One (2016) 11:e0158171. Abstract/Full Text
Surface-Exposed Lipoproteins: An Emerging Secretion Phenomenon in Gram-Negative Bacteria.
Wilson MM, Bernstein HD.
Trends Microbiol (2016 Mar) 24:198-208. Abstract/Full Text
Looks can be deceiving: recent insights into the mechanism of protein secretion by the autotransporter pathway.
Bernstein HD.
Mol Microbiol (2015 Jul) 97:205-15. Abstract/Full Text
Analysis of the outer membrane proteome and secretome of Bacteroides fragilis reveals a multiplicity of secretion mechanisms.
Wilson MM, Anderson DE, Bernstein HD.
PLoS One (2015) 10:e0117732. Abstract/Full Text
Charge-dependent secretion of an intrinsically disordered protein via the autotransporter pathway.
Kang'ethe W, Bernstein HD.
Proc Natl Acad Sci U S A (2013 Nov 5) 110:E4246-55. Abstract/Full Text
Cell biology: All clear for ribosome landing.
Bernstein HD.
Nature (2012 Dec 13) 492:189-91. Abstract/Full Text
Molecular basis for the activation of a catalytic asparagine residue in a self-cleaving bacterial autotransporter.
Barnard TJ, Gumbart J, Peterson JH, Noinaj N, Easley NC, Dautin N, Kuszak AJ, Tajkhorshid E, Bernstein HD, Buchanan SK.
J Mol Biol (2012 Jan 6) 415:128-42. Abstract/Full Text
The translational regulatory function of SecM requires the precise timing of membrane targeting.
Yap MN, Bernstein HD.
Mol Microbiol (2011 Jul) 81:540-53. Abstract/Full Text
Secretion of a bacterial virulence factor is driven by the folding of a C-terminal segment.
Peterson JH, Tian P, Ieva R, Dautin N, Bernstein HD.
Proc Natl Acad Sci U S A (2010 Oct 12) 107:17739-44. Abstract/Full Text
The conformation of a nascent polypeptide inside the ribosome tunnel affects protein targeting and protein folding.
Peterson JH, Woolhead CA, Bernstein HD.
Mol Microbiol (2010 Oct) 78:203-17. Abstract/Full Text
Molecular basis for the structural stability of an enclosed β-barrel loop.
Tian P, Bernstein HD.
J Mol Biol (2010 Sep 17) 402:475-89. Abstract/Full Text
Interaction of an autotransporter passenger domain with BamA during its translocation across the bacterial outer membrane.
Ieva R, Bernstein HD.
Proc Natl Acad Sci U S A (2009 Nov 10) 106:19120-5. Abstract/Full Text
The plasticity of a translation arrest motif yields insights into nascent polypeptide recognition inside the ribosome tunnel.
Yap MN, Bernstein HD.
Mol Cell (2009 Apr 24) 34:201-11. Abstract/Full Text
Incorporation of a polypeptide segment into the beta-domain pore during the assembly of a bacterial autotransporter.
Ieva R, Skillman KM, Bernstein HD.
Mol Microbiol (2008 Jan) 67:188-201. Abstract/Full Text
Autotransporter structure reveals intra-barrel cleavage followed by conformational changes.
Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK.
Nat Struct Mol Biol (2007 Dec) 14:1214-20. Abstract/Full Text
Are bacterial 'autotransporters' really transporters?
Bernstein HD.
Trends Microbiol (2007 Oct) 15:441-7. Abstract/Full Text
Cleavage of a bacterial autotransporter by an evolutionarily convergent autocatalytic mechanism.
Dautin N, Barnard TJ, Anderson DE, Bernstein HD.
EMBO J (2007 Apr 4) 26:1942-52. Abstract/Full Text
Protein secretion in gram-negative bacteria via the autotransporter pathway.
Dautin N, Bernstein HD.
Annu Rev Microbiol (2007) 61:89-112. Abstract/Full Text
The surprising complexity of signal sequences.
Hegde RS, Bernstein HD.
Trends Biochem Sci (2006 Oct) 31:563-71. Abstract/Full Text
Translation arrest requires two-way communication between a nascent polypeptide and the ribosome.
Woolhead CA, Johnson AE, Bernstein HD.
Mol Cell (2006 Jun 9) 22:587-98. Abstract/Full Text
An unusual signal peptide extension inhibits the binding of bacterial presecretory proteins to the signal recognition particle, trigger factor, and the SecYEG complex.
Peterson JH, Szabady RL, Bernstein HD.
J Biol Chem (2006 Apr 7) 281:9038-48. Abstract/Full Text
Efficient secretion of a folded protein domain by a monomeric bacterial autotransporter.
Skillman KM, Barnard TJ, Peterson JH, Ghirlando R, Bernstein HD.
Mol Microbiol (2005 Nov) 58:945-58. Abstract/Full Text
An unusual signal peptide facilitates late steps in the biogenesis of a bacterial autotransporter.
Szabady RL, Peterson JH, Skillman KM, Bernstein HD.
Proc Natl Acad Sci U S A (2005 Jan 4) 102:221-6. Abstract/Full Text
Last Reviewed August 2024