Publications

A selection of recent and significant publications can be viewed below.

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Hepatitis C virus infection dynamics, treatment, and lipid nanoparticle-mediated infection in humanized liver chimeric mouse models.

Park SB, Sánchez-Meza J, Umarova R, Shields J, Collignon L, Sula X, Holmbeck K, Oh JH, Farci P, Tyrrell DL, Feld JJ, Bukh J, Rice CM, Liang TJ.

Sci Adv (2026 May 29) 12:eaef1160. Abstract/Full Text

A genome-wide siRNA screen identifies previously unknown proviral and antiviral host factors in HBV infection.

Cheng X, Xia Y, Li C, Zhang M, Inuzuka T, Chakka S, Chen Q, Teng Y, Mao J, Li X, Dong W, Mouzannar K, Chih-Chien Cheng K, Liang TJ.

J Hepatol (2026 May) 84:874-886. Abstract/Full Text

A CRISPR-based genome-wide loss-of-function screen defines a role of host metabolism in regulating hepatitis B virus infection.

Inuzuka T, Mouzannar K, Zhang M, Umarova R, Park SB, Uchida T, Ma CD, Liang TJ.

Mol Ther (2026 Mar 4) 34:1616-1632. Abstract/Full Text

Targets of protective immunity and opportunities in hepatitis C virus vaccine development.

Park SB, Zimmer-Harwood P, Liang TJ.

Nat Rev Immunol (2026 Feb) 26:112-128. Abstract/Full Text

Nucleolin binds to and regulates transcription of hepatitis B virus covalently closed circular DNA minichromosome.

Xia Y, Cheng X, Nilsson T, Zhang M, Zhao G, Inuzuka T, Teng Y, Li Y, Anderson DE, Holdorf M, Liang TJ.

Proc Natl Acad Sci U S A (2023 Dec 5) 120:e2306390120. Abstract/Full Text

Additional Publications

Repurposing of lonafarnib as a treatment for SARS-CoV-2 infection.

Khan M, Irvin P, Park SB, Ivester HM, Ricardo-Lax I, Leek M, Grieshaber A, Jang ES, Coutermarsh-Ott S, Zhang Q, Maio N, Jiang JK, Li B, Huang W, Wang AQ, Xu X, Hu Z, Zheng W, Ye Y, Rouault T, Rice C, Allen IC, Liang TJ.

JCI Insight (2025 Jan 9) 10. Abstract/Full Text

Hepatitis B virus genotypes A1 and A2 have distinct replication phenotypes due to polymorphisms in the HBx gene.

Zhang M, Mouzannar K, Zhang Z, Teraoka Y, Piotrowski J, Ishida Y, Tateno-Mukaidani C, Saito T, Abe-Chayama H, Chayama K, Liang TJ.

PLoS Pathog (2025 Jan) 21:e1012803. Abstract/Full Text

How a medical mystery led to a cure for viral hepatitis.

Liang TJ.

Nat Med (2025 Jan) 31:8. Abstract/Full Text

Stem Cell-Derived Hepatocyte-Like Cells for Hepatitis B Virus Infection.

Xia Y, Park SB, Liang TJ.

Methods Mol Biol (2024) 2837:1-9. Abstract/Full Text

SARS-CoV-2 omicron variants harbor spike protein mutations responsible for their attenuated fusogenic phenotype.

Park SB, Khan M, Chiliveri SC, Hu X, Irvin P, Leek M, Grieshaber A, Hu Z, Jang ES, Bax A, Liang TJ.

Commun Biol (2023 May 24) 6:556. Abstract/Full Text

A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells.

Park SB, Uchida T, Tilson S, Hu Z, Ma CD, Leek M, Eichner M, Hong SG, Liang TJ.

Mol Ther Nucleic Acids (2022 Jun 14) 28:656-669. Abstract/Full Text

Targeting the Fusion Process of SARS-CoV-2 Infection by Small Molecule Inhibitors.

Park SB, Irvin P, Hu Z, Khan M, Hu X, Zeng Q, Chen C, Xu M, Leek M, Zang R, Case JB, Zheng W, Ding S, Liang TJ.

mBio (2022 Feb 22) 13:e0323821. Abstract/Full Text

Modeling PNPLA3-Associated NAFLD Using Human-Induced Pluripotent Stem Cells.

Tilson SG, Morell CM, Lenaerts AS, Park SB, Hu Z, Jenkins B, Koulman A, Liang TJ, Vallier L.

Hepatology (2021 Dec) 74:2998-3017. Abstract/Full Text

Infection courses, virological features and IFN-α responses of HBV genotypes in cell culture and animal models.

Zhang M, Zhang Z, Imamura M, Osawa M, Teraoka Y, Piotrowski J, Ishida Y, Sozzi V, Revill PA, Saito T, Chayama K, Liang TJ.

J Hepatol (2021 Dec) 75:1335-1345. Abstract/Full Text

IL-27-producing B-1a cells suppress neuroinflammation and CNS autoimmune diseases.

Choi JK, Yu CR, Bing SJ, Jittayasothorn Y, Mattapallil MJ, Kang M, Park SB, Lee HS, Dong L, Shi G, Caspi RR, Egwuagu CE.

Proc Natl Acad Sci U S A (2021 Nov 23) 118. Abstract/Full Text

Genetically edited hepatic cells expressing the NTCP-S267F variant are resistant to hepatitis B virus infection.

Uchida T, Park SB, Inuzuka T, Zhang M, Allen JN, Chayama K, Liang TJ.

Mol Ther Methods Clin Dev (2021 Dec 10) 23:597-605. Abstract/Full Text

Controlled Human Infection Model - Fast Track to HCV Vaccine?

Liang TJ, Feld JJ, Cox AL, Rice CM.

N Engl J Med (2021 Sep 23) 385:1235-1240. Abstract/Full Text

Discovery and Optimization of a 4-Aminopiperidine Scaffold for Inhibition of Hepatitis C Virus Assembly.

Rolt A, Talley DC, Park SB, Hu Z, Dulcey A, Ma C, Irvin P, Leek M, Wang AQ, Stachulski AV, Xu X, Southall N, Ferrer M, Liang TJ, Marugan JJ.

J Med Chem (2021 Jul 8) 64:9431-9443. Abstract/Full Text

Randomized Trial of a Vaccine Regimen to Prevent Chronic HCV Infection.

Page K, Melia MT, Veenhuis RT, Winter M, Rousseau KE, Massaccesi G, Osburn WO, Forman M, Thomas E, Thornton K, Wagner K, Vassilev V, Lin L, Lum PJ, Giudice LC, Stein E, Asher A, Chang S, Gorman R, Ghany MG, Liang TJ, Wierzbicki MR, Scarselli E, Nicosia A, Folgori A, Capone S, Cox AL.

N Engl J Med (2021 Feb 11) 384:541-549. Abstract/Full Text

HBV-Induced Increased N6 Methyladenosine Modification of PTEN RNA Affects Innate Immunity and Contributes to HCC.

Kim GW, Imam H, Khan M, Mir SA, Kim SJ, Yoon SK, Hur W, Siddiqui A.

Hepatology (2021 Feb) 73:533-547. Abstract/Full Text

Fluoxazolevir inhibits hepatitis C virus infection in humanized chimeric mice by blocking viral membrane fusion.

Ma CD, Imamura M, Talley DC, Rolt A, Xu X, Wang AQ, Le D, Uchida T, Osawa M, Teraoka Y, Li K, Hu X, Park SB, Chalasani N, Irvin PH, Dulcey AE, Southall N, Marugan JJ, Hu Z, Chayama K, Frankowski KJ, Liang TJ.

Nat Microbiol (2020 Dec) 5:1532-1541. Abstract/Full Text

A Viral Exposure Signature Defines Early Onset of Hepatocellular Carcinoma.

Liu J, Tang W, Budhu A, Forgues M, Hernandez MO, Candia J, Kim Y, Bowman ED, Ambs S, Zhao Y, Tran B, Wu X, Koh C, Surana P, Liang TJ, Guarnera M, Mann D, Rajaure M, Greten TF, Wang Z, Yu H, Wang XW.

Cell (2020 Jul 23) 182:317-328.e10. Abstract/Full Text

Chlorcyclizine Inhibits Viral Fusion of Hepatitis C Virus Entry by Directly Targeting HCV Envelope Glycoprotein 1.

Hu Z, Rolt A, Hu X, Ma CD, Le DJ, Park SB, Houghton M, Southall N, Anderson DE, Talley DC, Lloyd JR, Marugan JC, Liang TJ.

Cell Chem Biol (2020 Jul 16) 27:780-792.e5. Abstract/Full Text

Is SARS-CoV-2 Also an Enteric Pathogen With Potential Fecal-Oral Transmission? A COVID-19 Virological and Clinical Review.

Ding S, Liang TJ.

Gastroenterology (2020 Jul) 159:53-61. Abstract/Full Text

Diminished hepatic IFN response following HCV clearance triggers HBV reactivation in coinfection.

Cheng X, Uchida T, Xia Y, Umarova R, Liu CJ, Chen PJ, Gaggar A, Suri V, Mücke MM, Vermehren J, Zeuzem S, Teraoka Y, Osawa M, Aikata H, Tsuji K, Mori N, Hige S, Karino Y, Imamura M, Chayama K, Liang TJ.

J Clin Invest (2020 Jun 1) 130:3205-3220. Abstract/Full Text

Interferon-stimulated gene 20 (ISG20) selectively degrades N6-methyladenosine modified Hepatitis B Virus transcripts.

Imam H, Kim GW, Mir SA, Khan M, Siddiqui A.

PLoS Pathog (2020 Feb) 16:e1008338. Abstract/Full Text

A randomized, proof-of-concept clinical trial on repurposing chlorcyclizine for the treatment of chronic hepatitis C.

Koh C, Dubey P, Han MAT, Walter PJ, Garraffo HM, Surana P, Southall NT, Borochov N, Uprichard SL, Cotler SJ, Etzion O, Heller T, Dahari H, Liang TJ.

Antiviral Res (2019 Mar) 163:149-155. Abstract/Full Text

Development of Direct-acting Antiviral and Host-targeting Agents for Treatment of Hepatitis B Virus Infection.

Xia Y, Liang TJ.

Gastroenterology (2019 Jan) 156:311-324. Abstract/Full Text

Discovery and characterization of a novel HCV inhibitor targeting the late stage of HCV life cycle.

Park SB, Boyer A, Hu Z, Le D, Liang TJ.

Antivir Ther (2019) 24:371-381. Abstract/Full Text

TM6SF2 Promotes Lipidation and Secretion of Hepatitis C Virus in Infected Hepatocytes.

Boyer A, Park SB, de Boer YS, Li Q, Liang TJ.

Gastroenterology (2018 Dec) 155:1923-1935.e8. Abstract/Full Text

Hepatitis B Virus Deregulates the Cell Cycle To Promote Viral Replication and a Premalignant Phenotype.

Xia Y, Cheng X, Li Y, Valdez K, Chen W, Liang TJ.

J Virol (2018 Oct 1) 92. Abstract/Full Text

N6-methyladenosine modification of hepatitis B virus RNA differentially regulates the viral life cycle.

Imam H, Khan M, Gokhale NS, McIntyre ABR, Kim GW, Jang JY, Kim SJ, Mason CE, Horner SM, Siddiqui A.

Proc Natl Acad Sci U S A (2018 Aug 28) 115:8829-8834. Abstract/Full Text

Preclinical Pharmacological Development of Chlorcyclizine Derivatives for the Treatment of Hepatitis C Virus Infection.

Rolt A, Le D, Hu Z, Wang AQ, Shah P, Singleton M, Hughes E, Dulcey AE, He S, Imamura M, Uchida T, Chayama K, Xu X, Marugan JJ, Liang TJ.

J Infect Dis (2018 May 5) 217:1761-1769. Abstract/Full Text

Hepatitis C in Injection-Drug Users - A Hidden Danger of the Opioid Epidemic.

Liang TJ, Ward JW.

N Engl J Med (2018 Mar 29) 378:1169-1171. Abstract/Full Text

Hepatitis B virus evades innate immunity of hepatocytes but activates cytokine production by macrophages.

Cheng X, Xia Y, Serti E, Block PD, Chung M, Chayama K, Rehermann B, Liang TJ.

Hepatology (2017 Dec) 66:1779-1793. Abstract/Full Text

Cellular microRNA networks regulate host dependency of hepatitis C virus infection.

Li Q, Lowey B, Sodroski C, Krishnamurthy S, Alao H, Cha H, Chiu S, El-Diwany R, Ghany MG, Liang TJ.

Nat Commun (2017 Nov 27) 8:1789. Abstract/Full Text

Development of an Aryloxazole Class of Hepatitis C Virus Inhibitors Targeting the Entry Stage of the Viral Replication Cycle.

He S, Li K, Lin B, Hu Z, Xiao J, Hu X, Wang AQ, Xu X, Ferrer M, Southall N, Zheng W, Aubé J, Schoenen FJ, Marugan JJ, Liang TJ, Frankowski KJ.

J Med Chem (2017 Jul 27) 60:6364-6383. Abstract/Full Text

Hepatitis C Virus Lipoviroparticles Assemble in the Endoplasmic Reticulum (ER) and Bud off from the ER to the Golgi Compartment in COPII Vesicles.

Syed GH, Khan M, Yang S, Siddiqui A.

J Virol (2017 Aug 1) 91. Abstract/Full Text

Hepatitis B Reactivation Associated With Immune Suppressive and Biological Modifier Therapies: Current Concepts, Management Strategies, and Future Directions.

Loomba R, Liang TJ.

Gastroenterology (2017 May) 152:1297-1309. Abstract/Full Text

Human stem cell-derived hepatocytes as a model for hepatitis B virus infection, spreading and virus-host interactions.

Xia Y, Carpentier A, Cheng X, Block PD, Zhao Y, Zhang Z, Protzer U, Liang TJ.

J Hepatol (2017 Mar) 66:494-503. Abstract/Full Text

Infection of Hepatocytes With HCV Increases Cell Surface Levels of Heparan Sulfate Proteoglycans, Uptake of Cholesterol and Lipoprotein, and Virus Entry by Up-regulating SMAD6 and SMAD7.

Zhang F, Sodroski C, Cha H, Li Q, Liang TJ.

Gastroenterology (2017 Jan) 152:257-270.e7. Abstract/Full Text

Hepatitis C Virus Frameshift/Alternate Reading Frame Protein Suppresses Interferon Responses Mediated by Pattern Recognition Receptor Retinoic-Acid-Inducible Gene-I.

Park SB, Seronello S, Mayer W, Ojcius DM.

PLoS One (2016) 11:e0158419. Abstract/Full Text

Hepatitis C virus depends on E-cadherin as an entry factor and regulates its expression in epithelial-to-mesenchymal transition.

Li Q, Sodroski C, Lowey B, Schweitzer CJ, Cha H, Zhang F, Liang TJ.

Proc Natl Acad Sci U S A (2016 Jul 5) 113:7620-5. Abstract/Full Text

Hepatitis B Virus-Induced Parkin-Dependent Recruitment of Linear Ubiquitin Assembly Complex (LUBAC) to Mitochondria and Attenuation of Innate Immunity.

Khan M, Syed GH, Kim SJ, Siddiqui A.

PLoS Pathog (2016 Jun) 12:e1005693. Abstract/Full Text

Virology: The X-Files of hepatitis B.

Liang TJ.

Nature (2016 Mar 17) 531:313-4. Abstract/Full Text

Discovery, Optimization, and Characterization of Novel Chlorcyclizine Derivatives for the Treatment of Hepatitis C Virus Infection.

He S, Xiao J, Dulcey AE, Lin B, Rolt A, Hu Z, Hu X, Wang AQ, Xu X, Southall N, Ferrer M, Zheng W, Liang TJ, Marugan JJ.

J Med Chem (2016 Feb 11) 59:841-53. Abstract/Full Text

Present and future therapies of hepatitis B: From discovery to cure.

Liang TJ, Block TM, McMahon BJ, Ghany MG, Urban S, Guo JT, Locarnini S, Zoulim F, Chang KM, Lok AS.

Hepatology (2015 Dec) 62:1893-908. Abstract/Full Text

Hepatic expression levels of interferons and interferon-stimulated genes in patients with chronic hepatitis C: A phenotype-genotype correlation study.

Noureddin M, Rotman Y, Zhang F, Park H, Rehermann B, Thomas E, Liang TJ.

Genes Immun (2015 Jul-Aug) 16:321-9. Abstract/Full Text

Engrafted human stem cell-derived hepatocytes establish an infectious HCV murine model.

Carpentier A, Tesfaye A, Chu V, Nimgaonkar I, Zhang F, Lee SB, Thorgeirsson SS, Feinstone SM, Liang TJ.

J Clin Invest (2014 Nov) 124:4953-64. Abstract/Full Text

Integrative functional genomics of hepatitis C virus infection identifies host dependencies in complete viral replication cycle.

Li Q, Zhang YY, Chiu S, Hu Z, Lan KH, Cha H, Sodroski C, Zhang F, Hsu CS, Thomas E, Liang TJ.

PLoS Pathog (2014 May) 10:e1004163. Abstract/Full Text