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Advanced Light Microscopy & Image Analysis Core

Jeff Reece, M.S., Director

View staff and contact information.

The mission of the Advanced Light Microscopy & Image Analysis Core (ALMIAC) is to provide NIDDK researchers and their collaborators with tools and training in the latest technologies and techniques associated with Light Microscopy (LM), particularly 3D fluorescence microscopy.

The ALMIAC is an educator and enabler rather than a service provider, so that users can fully absorb and apply the concepts to their research projects, while gaining valuable hands-on experience.

To fulfill its mission, the NIDDK ALMIAC provides these resources:

  • ALMIAC microscopes:
    • Widefield: fluorescence, and color brightfield (for IHC slides).
    • Point-scanning confocal.
    • Spinning disk confocal, optionally with super-resolution via Image Scanning Microscopy (ISM).
    • Total internal reflection fluorescence (TIRF).
    • Super-resolution via single-molecule localization microscopy (SMLM).
    • Confocal Raman.
  • High-end image analysis workstations.
  • Floating software license for Huygens deconvolution software.
  • Training on the above, usually via scheduled one-on-one sessions.
  • Network file server for long-term storage of images, with support from CTB (Computer Technology Branch).
  • Connection to resources outside NIDDK ALMIAC, when the ALMIAC does not have the optimal equipment. Almost always when this situation arises, the equipment can be found elsewhere on the NIH Bethesda campus.
  • Support for NIDDK labs/branches that have or need LM equipment (outside the ALMIAC). Examples: advice on new imaging equipment purchases, including the paperwork side; and troubleshooting older imaging equipment.

If you work at NIDDK and wish to use ALMIAC resources, please contact Jeff Reece.