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Advanced Light Microscopy & Image Analysis Core

Jeff Reece, M.S., Director

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The relevance of light microscopy (LM) to biomedical research is embodied by two Noble Prizes in the past two decades —one for fluorescent proteins, and the other for super-resolution-- that are essentially subsets of fluorescence microscopy, the most malleable and popular form of LM. The mission of the NIDDK Advanced Light Microscopy & Image Analysis Core (ALMIAC) is thus to provide NIDDK researchers and their collaborators with tools and training in the latest technologies and techniques associated with LM, particularly 3D fluorescence microscopy. The ALMIAC is an educator and enabler rather than a service provider, so that users can fully absorb and apply the concepts to their research projects, while gaining valuable hands-on experience.

To fulfill its mission, the NIDDK ALMIAC provides these resources:

  • Instrumentation and training to those NIDDK researchers that do not otherwise have easy access to both. Training is usually via scheduled one-on-one sessions. Current microscopes include:
    • Widefield: fluorescence, and/or color brightfield
    • Point-scanning confocal
    • Spinning disk confocal
    • Total internal reflection fluorescence (TIRF)
    • Super-resolution via single-molecule localization microscopy (SMLM)
    • Confocal Raman
  • High-end image analysis workstations, and a network file server for long-term storage of images.
  • Support for NIDDK labs/branches that have or need LM equipment. For example, the ALMIAC expertise can be useful when making new imaging equipment purchases; and troubleshooting older imaging equipment.
  • Liaison to equipment outside NIDDK ALMIAC, when the ALMIAC does not have the optimal equipment. Almost always when this situation arises, the equipment can be found elsewhere on the NIH Bethesda campus.

If you work at NIDDK and wish to use ALMIAC resources, please contact Jeff Reece